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1	Animal Laboratories	ABS-2
The Effect Of Honey In Liver Histopatology View On Mice (Mus musculus) Induced By Monosodium Glutamate (MSG) Milawarni, Dwi Kesuma Sari, Muhammad Fadhlullah Mursalim, Shelly Salmah, Sukamto S. Mamada Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar, 90245, Indonesia Department of Histology, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar, 90245, Indonesia Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar , 90245 , Indonesia Email: milawarni15[at]yahoo.com Abstract Monosodium glutamate (MSG) is a commonly used flavoring agent, but excessive consumption of MSG can damage the liver so the liver requires exogenous antioxidants such as honey. This study aims to determine the effect of honey on histopathology images of liver mice after MSG administration. Research method with descriptive experimental. This study was conducted on 24 male mice divided into 4 groups. The control group, P1, P2, P3 were given an oral MSG of 4 mg / g BB. After 60 min, P2 and P3 were given peroral honey with a concentration of 5% at P2 and a concentration of 6% on P3 for 14 consecutive days. On the 15th day all of the dinecrops and liver mice were taken for preparing preparations using the method of embedding blocking with paraffin and hematoxylin eosin (HE) staining and analyzed descriptively qualitatively. The results showed that the treatment group 1 (KP 1) given MSG alone suffered more severe damage than the treatment groups 2 and 3 were also given honey 5% and 6%. Hepatocyte cell damage in all groups was fat degeneration, hydrophic degenration and necrosis. Histopathologic picture in treatment group 1 (KP 1) ie one field of view found more than 75% degeneration and necrosis on the observed part (severe damage). In the treatment group 2 (KP 2), one field of vision was found to be 21-50% degeneration and necrosis in the observed area. In the treatment group 3 (KP 3), one field of vision was found to be 21-50% degeneration and necrosis in the observed area. 5% and 6% concentration of honey concentrations can inhibit the liver damage of mice induced MSG mice. Keywords: Histopathology, honey, liver, mice, monosodium glutamate. Share Link | Plain Format | Corresponding Author (Milawarni )

2	Animal Laboratories	ABS-22
Detection of osteoporosis in female ovariohysterectomy and male orchiectomy rats based on blood calcium levels and bone histopathology Dian Fatmawati (a), Ilham Akbar (b), Irfan Idris (b), Dwi Kesuma Sari (a) a) Program Studi Kedokteran Hewan, Fakultas Kedokteran Universitas Hasanuddin b) Fakultas Kedokteran Universitas Hasanuddin Abstract The aims of this study is to identify an osteoporosis in ovariohisterectomy female rats and orchiectomy male rats, based on calcium levels and by looking at the bone histopathology. The male whistar rats and female whistar rats were used as a sample in this study. The 24 rats samples were divided into 4 treatment groups, P0, P1, P2 and P3 group. P0 group did not have ovariohysterectomy treatment and P1 group did not have orchiectomy treatment, while P2 group for ovariohysterectomy rats and P3 group for orchiectomy rats. All groups of rat were given standard feed and observed for 6 weeks after orchiectomy and ovariohisterectomy. Rats were placed in the same room with the same temperature, environment and feed. After 6 weeks, blood samples were taken from all rats and bones were taken for histopathological examination. Keywords: ovariohisterectomy, orchiectomy, osteoporosis Share Link | Plain Format | Corresponding Author (Dian Fatmawati)

3	Animal Laboratories	ABS-32
Histology of Sulawesi Medaka Fish Skin (Oryzias celebensis) in the Process of Healing Incision Wounds Jessica Tania Loto, Dr. drh. Dwi Kesuma Sari, AP.Vet, Dr. Irma Andriani, S.Pi, M.Si Hasanuddin University Abstract This study aims to determine the histology description of Sulawesi medaka (Oryzias celebensis) skin in the process of healing incision wounds. This research uses medaka fish which is a very famous experimental animal and has been widely used by researchers in the world to study in various fields of science, especially in biology and medicine. The samples studied were divided into 3 groups, namely group 1 as a negative control group (KP 1) without treatment as many as 6 Sulawesi medaka fish, group 2 namely the incision treatment group (KP 2) as much as 6 tails, group 3 as an incision treatment group and giving 6 Methylene blue (KP 3). After that the fish are kept in a 1 liter container and then maintained for 7 days. On the 7th day, all fishes were put into formalin to make preparations using Embedding blocking method with paraffin and Hematoxylin Eosin (HE) staining and analyzed descriptively qualitatively. Macroscopic observations showed that on day 1st the incision wound in the fish appeared reddish at the bottom of the wound. On the 7th day the incision wound was closed and the redness was not visible and there was no swelling for a period of 7 days. Microscopic observations showed that on day 7th an incision wound in fish showed signs of healing with improved skin epithelial forms. Keywords: fish, histology, medaka, Oryzias celebensis, skin Share Link | Plain Format | Corresponding Author (Jessica Tania Loto)

4	Animal Laboratories	ABS-74
Histopathology of small gut (duodenum) of White Rat (Rattus Norvegicus) post ovariohysterectomy and orchiectomy W S Monica (*), D K Sari, D Fatmawati Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, South Sulawesi, Indonesia *santawaode[at]med.unhas.ac.id Abstract This study aimed to determine the histopathological of the small intestine (duodenum) of white rats (Rattus norvegicus) after ovariohysterectomy and orchiectomy. In this study, 24 rats were used, consisting of 12 males and 12 females with a body weight ranging from 200-250 grams. The rat samples used were divided into four groups: the P0v group consists of the animals without ovariohysterectomy, the P1v group consists of animals with ovariohysterectomy, the P2r group consists of the animals without orchiectomy, and the P3r group consists of the animals with orchiectomy. Animals were adapted for one week, and then the animals were operated to remove the ovaries and testes. All groups were given the same feed or standard rat feed and observed for six weeks. Animals were euthanized for the small intestine (duodenum) sampling and then histology preparation was conducted using hematoxylin-eosin staining. Keywords: histopathology, duodenum, Rattus norvegicus, ovariohysterectomy, orchiectomy Share Link | Plain Format | Corresponding Author (Wa Ode Santa Monica)

5	Animal Laboratories	ABS-75
The Effect Of Giving Patchouli Oil (Oleum Pogostemon Cablin Benth) On The Mortality Time Of Boophilus Microplus Tick In Cow M Khair 1, W S Monica2, A Ris 3, A W Jamaluddin 4 and M Nur5 Universitas Hasanuddin Abstract This study aimed to determine patchouli oil^s effect on the mortality time of Boophilus microplus ticks in cattle. Materials and methods in this study used 24 ticks and divided into eight groups: the positive control group (K +) used cypermethrin, the negative control group (K-) used hexane, Patchouli Oil 5% Group (MN 5%), Patchouli Oil 5% Interval Group (MN 5% Int), Patchouli Oil 10% Group (MN 10%), Patchouli Oil 10% Interval Group (MN 10% Int), Patchouli Oil 20% Group (MN 20%), and Patchouli Oil 20% Interval Group (MN 20% Int) six groups used patchouli oil with different concentrations and treated for 6 hours in vitro. The results showed that the K+ group tick started to die in the first hours while the K- group there were no tick deaths. The MN 5% group of the deaths occurred at the fourth hours, the MN 5% Int group deaths happened at the fourth hours, MN 10% Group had deaths at the third hours, MN 10% Int Group died of ticks at three hours, and MN 20% and MN 20% Int Group killed at the first hours, with a total death is three tick each group. Giving patchouli oil to see the mortality rate of the ticks carried out in vitro has a good effect or can kill the ticks at a concentration of 5%, 10%, and 20%, with the highest time of tick death occurring at a concentration of 20%, namely in the MN 20% and MN 20% Int groups. Keywords: Patchouli oil, Pogostemon Cablin Benth Share Link | Plain Format | Corresponding Author (miftahul khair)

6	Animal Laboratories	ABS-76
UVB radiation induces skin inflammation and changes in bone marrow cell population on rats Verda Farida (a), Dion Adiriesta Dewananda (b), Muhammad Novrizal Abdi Sahid (c), Siti Isrina Oktavia Salasia (b*) a) Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Ahmad Dahlan, Jl. Prof. Dr. Soepomo, S.H., Yogyakarta 55164, Indonesia b) Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia *isrinasalasia[at]ugm.ac.id c) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jl. Sekip Utara, Yogyakarta 55281, Indonesia Abstract Ultraviolet B (UVB) radiation is commonly used to study skin-related inflammation mediated by skin-resident immune cells. These cells are mobile and can release various pro-inflammatory proteins that might affect blood cells that not reside in the skin. This study aimed to investigate the skin condition and global blood cell condition after UVB radiation. Ten rats were divided equally into two groups, where the first group (K1) as control and the second group (K2) was exposed to UVB within 30 minutes/day for consecutive nine days. Prior to radiation, the rats were shaved at the back with size 2 x 2 cm. After the final UVB radiation blood samples from bone marrow were collected, proceed for Giemsa staining. The skin tissues were processed for Hematoxylin and Eosin, Tuloidin blue, and Mallory staining. Cornification, pigmentation, collagen-density depletion, haemorrhage were only observed in the skin tissues of group with UVB radiation. The erythroid and myeloid ratio was increased to around 1.6:11.2 from the normal ratio of 1:4. In addition, the leukocytes number is increased from 8.37x103 cells/mm3 (normal group) to 14.396x103 cells/mm3. These results show that UVB radiation affected not only skin-resident cells but also blood cells. Keywords: UVB- radiation- rat- skin- inflammation- haematopoiesis Share Link | Plain Format | Corresponding Author (Verda Farida)

7	Animal Laboratories	ABS-85
Comparison between Sulawesi Medaka Fish (Oryzias celebensis) and Medaka Fish (Oryzias latipes): morphological and histological studies Irma Andriani, Khusnul Yaqin, Dwi Kesuma Sari 1. Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University 2. Faculty of Marines and Fisheries Sciences, Hasanuddin University 3. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University Abstract The Sulawesi Medaka Fish (Oryzias celebensis) is an endemic fish that only exists in the South Sulawesi region. The use of Sulawesi medaka fish as experimental animals as well as Medaka fish or rice fish originating from Japan has begun to develop at this time. Research has been carried out to see the comparison between the two types of fish. Observations between the two types of fish were carried out by observing the morphology and histology of various organs. The differences that were found were not too significant, so it is possible to use the Sulawesi Medaka fish, especially as model animals, as has been done with the Medaka fish. Keywords: Sulawesi Medaka fish, Medaka fish, rice fish, comparison Share Link | Plain Format | Corresponding Author (Irma Andriani)

8	Animal Laboratories	ABS-94
Histology of Sulawesi Medaka Fish Skin (Oryzias celebensis) in the Burn Healing Process Andi Azifah Cahyani, Dwi Kesuma Sari, Irma Andriani Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, South Sulawesi, Indonesia Abstract Medaka fish is a group of fish from the Actinopterygii class. One of the endemic medaka fish of South Sulawesi is the Sulawesi medaka fish Oryzias celebensis. Medaka fish are widely known as model fish in biology. This study aims to determine the histological description of the skin of the Sulawesi medaka in the healing process of burns. This study used the Sulawesi medaka fish which is a fish endemic to Sulawesi. The fish samples studied were divided into 3groups, group 1 as the control group(K1), group 2 namely the burn treatment group (K2) and then group3 (K3) namely a group of fish that were treated with burns and then kept in a container whose water has been dripped with methyleneblue. The fish in groups2 and 3 would then observe the wound healing process for 7days, namely on the 1st,3rd,5th, and 7th day. The fish were then immersed in formalin for 2days and then made preparations with the embedding blocking method with paraffin and HematoxylinEosin (HE) staining and analyzed descriptively qualitatively. Macroscopic observations of fish in the treatment group after giving burns (K2) on day 1 showed signs of widespread redness and the wound was still visible. On the 3rd,5th and 7th day there were no signs of redness and the wounds began to shrink on day 7.The results of observations in the treatment group with burns treatment coupled with the administration of methyleneblue (K3) on day 1 showed signs of redness and the wound is still visible. Day 3 the wound is not red and the wound is medium in size. On the5th day the wound started to get smaller and showed no signs of redness and swelling while on the7th day the redness was gone and the wound had begun to close. Observation of the histological features of the skin of the Sulawesi Medaka fish showed differences on day 1 to day 7. Inflammatory cells, fibroblast cells were found from day 1 to day7 with increasing numbers. Collagen fibers were found more on the 7th day while on the 7th day only granulation tissue. Keywords: skin, medaka, Oryzias celebensis, histology, burn wound Share Link | Plain Format | Corresponding Author (Andi Azifah Cahyani)

9	Animal Laboratories	ABS-97
Care Management and Health Monitoring of Laboratory Guinea Pigs at Animal House of Faculty of Biology, Gadjah Mada University Laksmindra Fitria(a*), Slamet Raharjo(b), Nastiti Wijayanti(a), Tuty Arisuryanti(a), Siti Isrina Oktavia Salasia(b) (a) Faculty of Biology, Gadjah Mada University, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281 * laksmindraf[at]ugm.ac.id (b) Faculty of Veterinary Medicine, Gadjah Mada University, Jalan Fauna No.2, Karang Malang, Yogyakarta 55281 Abstract Guinea pigs or Cavia porcellus (Linnaeus, 1758) is one of laboratory animals commonly used in biomedical research. Although not as popular as rats and mice, guinea pigs actually having more physiological similarities to humans, one of which is the inability to synthesize endogenous vitamin C. In Indonesia, the use of this animal in research is limited as animal facilities that provide laboratory guinea pigs are rare. Therefore, we conducted this study to initiate the provision of laboratory guinea pigs. Individual for starters were obtained from conventional farming, and then acclimatized at the Animal House of Faculty of Biology GMU. Acclimatization is a crucial step because animals will be challenged to undergo laboratory lifestyles. These changes can cause stress that impacts on the health. Care management in accordance with The Laboratory Guinea Pigs reference book, consisted of: housing, handling, diet, environmental conditions, sanitation, and health monitoring. Modifications were made to adjust the conditions refer to the principles of animal welfare and ethics. Results showed that conventional guinea pigs can be raised for laboratory guinea pigs after acclimatization and controlled breeding. Physiological disorders found during acclimatization particularly vitamin C deficiency and loss of appetite due to diet changes. Health problems mainly affected skin especially parasitic disease such as scabies and infection related to environmental hygiene such as fungal infection or dermatophytosis. The medical treatments were administration of antiparasitic such ivermectine combine with antiinflamatory and vitamins, antifungal such as ketoconazole or miconazole topically. Keywords: guinea pigs, laboratory animals, care management, health problems Share Link | Plain Format | Corresponding Author (Laksmindra Fitria)

10	Animal Laboratories	ABS-98
The Effect Of Aromatherapy Of Curcuma Essential Oil (Curcuma xanthorrhiza L) On The Level Of Triglyceride Blood Of White Rats That Feed With High-Fat N Safitri1, W S Monica2, A W Jamaluddin 3, Y M Adikurniawan 4 and M N Amir 5 1Veterinary Medicine Study Program, Hasanuddin University, Makassar, South Sulawesi, Indonesia 2Division of Veterinary Internal Medicine, Veterinary Medicine Study Program Hasanuddin University, Makassar, South Sulawesi, Indonesia 3Division of Veterinary Pharmacy, Veterinary Medicine Study Program, Hasanuddin University, Makassar, South Sulawesi, Indonesia 4Division of Veterinary Surgery and Radiology, Veterinary Medicine Study Program, Hasanuddin University, Makassar, South Sulawesi, Indonesia 5Faculty of Pharmacy, Hasanuddin University, Makassar, South Sulawesi, Indonesia Abstract This study aims to determine the effect of aromatherapy essential oil Curcuma on triglyceride levels of rats fed high-fat feed. The materials and materials of this study used 15 white rats divided into five treatment groups: the healthy control group (KKS) was only given standard feed, the negative control group (KKN) was only given high-fat feed (cow brain suspension), the positive control group (KKP) given high-fat feed and simvastatin, treatment group 1 (KP1) was given 0.1 ml of Curcuma essential oil aromatherapy, and treatment group 2 (KP2) was given a dose of 0.5 ml. therapy using aromatherapy to white rats was given for 15 days with a time of 10 minutes per day. The results showed that giving aromatherapy essential oil of Curcuma can reduce blood triglyceride levels in the KKS, KKN, KKP, KP1, and KP2 groups respectively by 3.94% (3.34 mg / dL), 11.89% (9 , 33 mg / dL), 38.06% (31.33 mg / dL), 49.17% (39.33 mg / dL), and 50% (45 mg / dL). the results of the study also showed weight loss in the KKS, KKN, KKP, KP1, and KP2 groups respectively of 8.62% (16.66 gr), 8.62% (16.66 gr), 10, 9% (23.34 gr), 3.08% (6.67 gr), and 24.19% (50 gr). Keywords: Curcuma Essential Oil (Curcuma xanthorrhiza L), Aromatherapy, White Rats Share Link | Plain Format | Corresponding Author (Nurul Safitri)

11	Animal Science	ABS-90
The Effect of Heart Infarction on Electrocardiogram value of Porcine (Sus scrofa) as a Model for Human Gunanti (a,1), Normalina Sandora (b,2) , Muhammad Arza Putra (c), Nur Amalina Fitria (b), Tyas Rahmah Kusuma (b), Melpa Susanti Purba (a), Arief Boediono (a) , Deni Noviana (a,d*) a) Faculty of Veterinary Medicine (FKH), IPB University, Bogor, 16680, Indonesia b)Human Reproduction, Infertility and Family Planning, Indonesia Medical Education and Research Institute (IMERI), Jakarta, 10430, Indonesia c)Department of Surgery, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta Pusat, 10430, Indonesia d)The Veterinary Medical Teaching Hospital (RSHP), IPB University, Bogor, 16680, Indonesia 1) First author 2) Co-author *Corresponding Author: deni[at]apps.ipb.ac.id Abstract Cardiovascular disease is the most cause of death, whilst one out of four dead in Indonesia was related to coronary heart disease, consuming health budget up to US-943 million in 2016. The obstruction of a coronary artery causes myocardial infarction. Electrocardiography (ECG) is a routine device to indicate electrical conduction of the heart. This study aims to evaluate the value of ECG after infarction of the heart. Elektrocardiography was performed before the induction of cardiac infarction as a normal result. The anesthesized porcine placed in right lateral recumbency. The table was insulated from metal surface using underpad. Five ECG electrodes placed on the 2 forelimbs, the 2 hindlimbs, and the sternum. The hair in those areas clipped beforehand. The tape and additional gel were used to improve contact between the patient and electrodes. The electrodes were connected to the ECG machine by color-coded cables. The occlusion performed in this study was at the 1 per 3 distal of the left anterior descendant (LAD). After 60 minutes surgery, the ECG was performed again as immediately 60 min after induction. After a month, we ECG wak taken again to see the value of application infarct in porcine. The T amplitude and ST wave 60 minutes after treatment was elevated. After first month observation, the ST elevation had disappeared and abnormal Q waves were found. Elektrocardiography of porcine after 60 minutes induction showed a typical myocardial infarct (MCI) and after a month showed the indication of myocardial hypoxia and myocardial infarction. Keywords: Electrocardiography, porcine myocardial infarction, left anterior descendant, ST elevation Share Link | Plain Format | Corresponding Author (Gunanti -)

12	Animal Welfare and Animal Ethics	ABS-20
Theoretical Study on Nonlinear The Impact of the Covid-19 Pandemic on the Slaughter of Qurban Animals in the Implementation of Mosque-Based Animal Welfare in West Nusa Tenggara Province Mashur (a*), Kholik(a), Dina Oktaviana (a), Supriadi (a),Candra Dwi Atma (a), Muhammad Munawaroh (a) aFaculty of Veterinary Medicine, Mandalika University of Education Abstract Pandemic situation of Covid-19 has been hit all aspects of human life. The Covid-19 pandemic also has an impact on the slaughter of sacrificial (quban) animals. The Muslims in Indonesia every year slaughter quban animal on the Eid al-Adha in the mosque. The slaughter of qurban animals in mosques is still done traditionally, paying less attention to aspects of hygienic, sanitation, animal health, animal welfare, environmental health, and zoonotic aspects. This study aims to determine the impact of Covid-19 on the slaughter of qurban animals in mosques in the implementation of animal welfare. This study used a survey method by distributing questionnaires online to animal qurban committees in mosques in West Nusa Tenggara. The sample was determined by using the opportunity sampling technique, which was based on the questionnaire returned by the sacrificial animal committee. 118 questioners were tabulated and analyzed descriptively. The results showed that 72.73% of people in West Nusa Tenggara felt the impact of Covid-19 on the slaughter of qurban animals, which was marked by a decrease in community income by 62.73%, people found it difficult to get jobs 16.36%, there was no assistance from qurban animals from the government 5 , 45% and an increase in the price of qurban animals by 7.27%. This has led to a decline in the number of qurban animals slaughtered in mosques by 11.50% during the Covid-19 pandemic in 2020. From the aspect of animal welfare that is slaughtered in mosques, 74.25% of qurban animals have their health checked before and after being slaughtered and 25.75. % of the qurban committee stated that they did not check the health of their animals because they did not know the inspection procedure 32.26%, there were no officers 24.73% and 43.01% stated that the habit of not checking the health of their animals every year. The conclusion is that the Covid -19 pandemic has had an impact on the decrease in the number of qurban animals slaughtered in mosques and Keywords: Animal welfare- Impact of covid-19- Mosques- Qurban animal Share Link | Plain Format | Corresponding Author (Mashur Mashur)

13	Biology and Biomedical Science	ABS-9
L-carnitine prevents hepatic steatosis in deep-frying oil-treated rat Risha Catra Pradhany (a), Ida Bagus Gde Rama Wisesa (b), Ferbian Milas Siswanto (c*) a) Study Program of Veterinary Medicine, Faculty of medicine, Hasanuddin University, Makassar 90245, Sulawesi Selatan, Indonesia b) Faculty of Veterinary Medicine, Brawijaya University, Malang 65145, East Java, Indonesia c) Department of Biotechnology, Dhyana Pura University, Badung 80361, Bali, Indonesia *ferbianms[at]undhirabali.ac.id Abstract Deep-frying oil is a source of free radicals that causes oxidative stress in the body and lead to chronic and degenerative diseases, including non-alcoholic fatty liver or NAFLD. Steatosis, or fatty liver, is one of NAFLD and is a common result of moderate to severe hepatocellular insult. L-Carnitine involves in the metabolism of fat and has a potential role as an antioxidant. The purpose of this study was to elucidate the role of L-Carnitine in deep-frying oil-treated rats. Thirty-six experimentally naive male Wistar albino rats (3-4 months old, weighing 180-200 g) were divided randomly into three groups: control untreated (C) group (n= 12, without any treatment), negative control (NC) group (n= 12, deep-frying oil of 0.42 ml/rat/day for 3 weeks), and L-Carnitine treatment (T) group (n= 12, deep-frying oil of 0.42 ml/rat/day, and L-Carnitine dose of 30 mg/kg/day for 3 weeks). The results showed that the number of hepatic steatosis in the NC group was higher than in both C and T groups. Furthermore, L-Carnitine significantly decreased hepatic TG content (p <0.01). L-Carnitine increased the expression of hepatic SOD, CAT and GPx (p <0.05). Additionally, the serum ALT and AST levels were significantly lower in the T group compared to the NC group, and both serum and hepatic MDA levels were also lower (p <0.01 for all). Thus, this study suggests that L-Carnitine prevents the development of hepatic steatosis and oxidative damage, as well as improves the hepatic antioxidant defenses. Keywords: Steatosis- deep-frying oil- oxidative stress- L-Carnitine- antioxidant Share Link | Plain Format | Corresponding Author (Ferbian Siswanto)

14	Biology and Biomedical Science	ABS-12
BISPHENOL A INCREASES MALONDIALDEHYDE AND APOPTOTIC INDEX IN THE LIVER OF MALE RATS Risha Catra Pradhany (a*), I Nyoman Suarsana (b), I Gusti Ayu Agung Suartini (b), Ferbian Milas Siswanto (c) a) Study Program of Veterinary Medicine, Hasanuddin University, Makassar 90245, Sulawesi Selatan, Indonesia *rishacatra[at]yahoo.com b) Department of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Denpasar 80234, Bali, Indonesia c) Department of Biotechnology, Dhyana Pura University, Badung 80361, Bali, Indonesia Abstract Bisphenol A is a well-known toxin whose effects on reproductive toxicities. However, recent years, it has also been known BPA causes oxidative stress. Oxidative stress is one of the factors causing organ damage, this study aims to prove the effect of oral bisphenol A administration on malondialdehyde levels and apoptotic index in rat liver. This study used a posttest only control group design. Subjects were 14 male Sprague Dawley rats, male, aged 8-10 weeks, body weight 180 grams, and healthy. The control group (P0), 7 rats, were given a placebo of 1 ml aquadest for 21 days- while the treatment group (P1), 7 rats, were given 400 mg/kgBW of BPA for 21 days. The results showed that the P1 group had higher hepatic MDA levels (3,33- 0,27 nmol/mg.prot) and significantly different (p<0,001) than the P0 group (2,67- 0,14 nmol/mg.prot). In addition, the P1 group had a higher apoptotic index (11,21- 2,26%) and significantly different (p<0,001) than the P0 group (2,19- 0,97%). Based on these results, it can be concluded that oral administration of bisphenol A increases malondialdehyde and apoptotic index in the liver of male Sprague dawley rats. Keywords: Bisphenol A- Malondialdehyde- Apoptotic Index- Liver Share Link | Plain Format | Corresponding Author (Risha Catra Pradhany)

15	Biology and Biomedical Science	ABS-27
Preliminary Phytochemical Analysis and Antibacterial Activity of Aqueous Extract of Cassia siamea Leaves Againts Aeromonas hydrophila Budianto Panjaitan1, Amalia Sutriana1*, Femi J. Sari1, Nuzul Asmilia1, Erina1 1Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia Abstract This study aims to investigate the phytochemical analysis and in vitro antibacterial activity of aqueous extract of Cassia siamea against fish pathogenic Aeromonas hydrophila. Aqueous extract of Cassia siamea leaves were prepared and preliminary screened for phytochemical analysis. The different concentrations of the extract were then tested for their antibacterial activity against Aeromonas hydrophila using agar well diffusion method which was performed on Mueller Hinton Agar (MHA) plates. The phytochemical analysis showed that the aqueous extract of Cassia siamea contained alkaloid, flavanoids, phenols, steroids, tannin. The antimicrobial activity analysis showed that aqueous extracts of Cassia siamea at concentration of 40 %, 50, 60%, and 70% were able to inhibit the growth of A. hydrophila bacteria with zones of inhibition ranging from 10.40\pm0.36 mm to 13.47\pm0.5 mm as the lowest and highest zones of inhibition. The result of this study have supported the potential use of Cassia siamea as the natural antibacterial and recommend the possibility use of Cassia siamea in the treatment of fish disease caused by A. hydrophila. Keywords: antibacterial activity, Cassia siamea, phytochemical screening Share Link | Plain Format | Corresponding Author (Budianto Panjaitan)

16	Biology and Biomedical Science	ABS-55
Phyllanthus emblica influence on the production capacity of the Interleukin 10 and Interleukin 12 in Mus musculus inoculated with Plasmodium berghei N Asmilia (a*), Y Fahrimal(b*), M Abrar(c*), Rinidar (d*), Rusli (a*), F Athailah (b*) and Sugito (a*) a*) Clinical Laboratory Veterinary Medicine, b*)Parasitology Laboratory, c*) Microbiology Laboratory, d*) Farmacology Laboratory Universitas Syiah Kuala Abstract Malaria is still a major problem for health throughout the world including Indonesia. Previous research on the antimalarial activity of ethanol extract of Malacca leaf using Plasmodium falciparum has shown excellent parasitemia inhibitory activity. The purpose of this study was to determine the effect of Phyllantus emblica ethanol extract on the production capacity of IL-10 and IL-12 cytokines in mice inoculated with Plasmodium berghei. This study was a laboratory experimental study using a post-test only control group design. The samples used were 20 male mice (Mus musculus) with body weight 20-25 grams, and aged 6-8 weeks. The infected mice were randomly divided into 5 treatment groups consisting of 4 mice. K0 as negative control group (given P. berghei without malacca leaf extract)- K1 as positive control group (given P. Berghei and chloroquin 5 mg / kg BW- groups K2, K3, and K4, were given P. berghei and malacca leaf extract at doses of 300, 600, and 1200 mg / kgBW. Administration of chloroquin and malacca leaf extract was carried out on the first day of the study 1 hour after the degree of parasitemia reached 30-40%. The administration of chloroquin and malacca leaf extract was every day for 4 consecutive days. The mice production capacity of IL-10 and IL-12 was measured using the Enzyme Linked Immunosorbent Assay (ELISA) method, then a microplate reader was used to read Optical Density (OD). Data were analyzed using one-way analysis of variance (ANOVA), followed by the Duncan test.The IL10 and IL-12 levels showed significant differences (p <0.05). The conclusion of this study is that Phyllantus emblica increased IL-12 production capacity and decreased IL-10 capacity, therefore, Phyllantus emblica could serve as an alternative antimalarial drug as it has protective role during malaria infection. Keywords: Phyllantus emblica, Plasmodium berghei, Interleukin 10, Interleukin 12 Share Link | Plain Format | Corresponding Author (Nuzul Asmilia)

17	Biology and Biomedical Science	ABS-57
Safety Evaluation of Antiparasitic Combination Afoxolaner and Milbemycin Oxime in Dog Tri Isyani Tungga Dewi1, Upik Kesumawati2, Susi Soviana2, Risky Andrea1, Nurul Annisa1, Hamdika Yendri1 , Deni Noviana1,3 1 Veterinary Teaching Hospital, Faculty of Veterinary Medicine, IPB University 2Division of Parasitology and Medical Entomology, Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University 3Division of Surgery and Radiology, Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University Abstract Antiparasitic treatment through oral application for both ectoparasites and endoparasites has a good level of efficacy. This study aimed to determine the safety of the oral anti-parasitic drug combination afoxolaner which is effective on ticks and fleas, and milbemycin oxime which is effective on nematodes. A total of three Beagles weighing 10 kg were given a combination of afoxolaner and milbemycin oxime 3 times the recommended dose (Afoxolaner 150 mg and Milbemycin oxime 30 mg). Clinical observations of the respiratory system, circulatory system and digestive system were carried out at 1, 2, 3 and 24 hours after drug administration. Examination of blood samples was also performed to determine the hematological profile. Blood samples were collected 2 times, namely before administering the drug and 24 hours after administration. The results of the study showed that there were no any clinical symptoms of intoxication after treatment. There were no abnormalities in the frequency, rhythm and type of breathing. The intensity, frequency and rhythm of the heart were still normal. Body temperature, capillary refill time values &#8203-&#8203-were in normal ranges category. Symptoms of abnormality in digestive system such as hypersalivation, vomiting and diarrhea were not found. Statistical analysis showed that there were no significant difference (p> 0.05) in the total number of erythrocytes, hemoglobin levels, hematocrit values, as well as erythrocyte index values. As conclusion, the combination of afoxolaner and milbemycin oxime as drug administration does not have any effect intoxication. Keywords: afoxolaner, dog, milbemycin oxime, safety evaluation Share Link | Plain Format | Corresponding Author (Tri Isyani Tungga Dewi)

18	Biology and Biomedical Science	ABS-58
Mangosteen Skin Extract (Garcinia mangostana L.) as anti-bacterial in Escherichia coli. Abdul Wahid Jamaluddin, A. Magfira Satya Apada, Pusphita Sari Corresponding author: abdulwahidjamaluddin[at]unhas.ac.id Abstract Mangosteen fruit skin with high antioxidant content has various potential as active medicinal substances, including antibacterial. Escherichia coli, which is commonly found in food and beverages and even animal feed, is ,of course a serious threat because in addition to the large issue of antibiotic resistance when using synthetic drugs, m E. coli can also cause foodborne diseases in the form of intestinal tissue necrosis, Hemorraghic colitis and Hemolytic uremic syndrome. For that we will test this potential mangosteen peel extract on E. coli bacteria and determine how its activity is to inhibit the growth of E. Coli. We use the following mangosteen rind extract concentrations for testing, namely 5%, 10%, 20% and 40% which was diluted using 10% DMSO then put into a blank disk, then the positive control used the antibiotic disk Amoxicillin plus clavulanate acid with a content of 30 micrograms and negative control using 10% DMSO. Testing was carried out in triple and tested on Escherichia coli bacteria using Muller Hinton Agar (MHA) media. The results of this study showed that the inhibition of E. coli starting from the extract of the skin for mangosteen 5% was 8.9 mm and the greatest inhibition was at a concentration of 40% of 16.8 mm. This mangosteen rind extract is effective in inhibiting the growth of the Escherichia coli bacteria. Keywords: Mangosteen Skin Extract (Garcinia mangostana L.), antibacterial activity, Escherichia coli, in vitro Share Link | Plain Format | Corresponding Author (Abdul Wahid Jamaluddin)

19	Biology and Biomedical Science	ABS-69
Platelet Rich Plasma (PRP) Therapy in Actopic Dermatitis Cases of dog at RSHP FKH IPB University Hamdika Yendri1, Tri Isyani Tungga Dewi1, Arief Boediono2, Mawar Subangkit3 , Andriyanto12, Deni Noviana13 1 Animal Teaching Hospital, Faculty of Veterinary Medicine, IPB University 2 Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University 3 Department of Clinical, Reproductive and Pathology, Faculty of Veterinary Medicine, IPB University Abstract Treatment using cell therapy has developed well in several years. Cell therapy can be used to treat degenerative diseases and can be an alternative treatment with minimal use of chemical compounds. This article aims to show the results of cell therapy in cases of actopic dermatitis in dogs at RSHP FKH IPB University. The dog named volley, four years old female dog with breed is bull dog comes with complaints of itching and rash all over the skin surface. Hair loss is also found on the back side area. The diagnosis is carried out by conducting a physical examination, examining skin scrapings, and blood tests. The result of physical examination found that there was a wound on the back due to scratching. Examination of skin scrapings did not reveal ectoparasite and fungal infestations. Blood tests result there are no abnormality in the haematology parameter. Based on the tests carried out, the diagnosis established for volley is actopic dermatitis. The therapy given is Platelet Rich Plasma (PRP) cell therapy using the patient^s blood. A total of 15 mL of patient blood was centrifuged to obtain section of Platelet Rich Plasma then that section was injected intravenously using 0.9% NaCl infusion fluid. This cell therapy is carried out three times one week apart. The results obtained were an improvement in the skin condition after three treatments. Cell therapy treatment in cases of dermatitis in dogs showed good results. Keywords: Keywords: Cell therapy, actopic dermatitis, Platelet Rich Plasma Share Link | Plain Format | Corresponding Author (Hamdika Yendri Putra)

20	Biology and Biomedical Science	ABS-101
YAP (Yes-Associated Protein) Dynamics in the Mouse Embryo Transition Phase using Fluorescence Intensity Data Diah Nugrahani Pristihadi (a), Noer Muhamad Dliyaul Haq (a), Dwi Budiono (a), Vista Budiariati (b), Rini Widyastuti (a,c), Annisa Fithri Lubis (a), Karisma Mardatillah (a), Mokhamad Fahrudin (a), Andriyanto (a), and Arief Boediono (a*) (a) Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, West Java, Indonesia- (b) Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Yogyakarta, Indonesia- (c) Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Animal Husbandry Faculty, Universitas Padjajaran, Bandung 45363, West Java, Indonesia- *Corresponding author: ab[at]apps.ipb.ac.id Abstract In this modern era, the conversion of ^images^ into numerical data was very possible to do. In this study, we have transformed immunofluorescence images into intensity data by utilizing the Zen software. Three sample groups of in vitro cultured mouse embryo throughout the embryonic transition phase (8-cell, morula, and blastocyst) were used to identify the YAP (Yes-associated protein) expression. YAP allegedly was the most important protein in this differentiation process. The prepared samples were observed using the z-stack imaging method by using an LSM confocal. By this method, we are allowed to measure the dynamics of YAP on each cell. The study results showed that the total YAP expressed equally on each cell, but envoy a different manifestation. YAP discovered filling the nuclear, cytoplasmic, or both sides. Intensity analysis of each type of cell showed a non-significant difference among embryo group. In conclusion, cells maintained the YAP equilibrium during the embryonic transitional process. Keywords: YAP- Mouse embryo- Fluorescence Share Link | Plain Format | Corresponding Author (Diah Nugrahani Pristihadi)

21	Marine Biology and Marine Disease	ABS-6
Analysis of Kidney Microanatomy of Tilapia Fish (Oreochromis mossambicus) Contaminated by Lead Metal (Pb) in Lake Tempe Wajo Regency A. Nur Indri Paramita (a), Dwi Kesuma Sari (b), Sharifuddin Bin Andy Omar (a) Hasanuddin University Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, Telp. (0411) 586010 Psw. 1. Fax. (0411) 586297. (b) Faculty of Medicine, Majoring in Veterinary Medicine. Abstract Tilapia fish (Oreochromis mossambicus) is a freshwater fish that is consumed by many people. The purpose of this study was to determine the Analysis of Kidney Microanatomy of tilapia fish (Oreochromis mossambicus) contaminated by Lead Metal (Pb) in Lake Tempe, Wajo Regency. The samples used were twenty-one tilapia fish with seven kidney samples in each station. Measurement of heavy metal content was carried out with Atomic Absorption. Kidney preparations were fixed using 10 percen neutral buffered formalin (NBF), dehydration using multilevel alcohol, embedding using paraffin, cutting with a thickness of 4 micrometer stained using haematoxillin eosin then observed. Data analysis used is qualitative descriptive. Based on the results of observations obtained by damage or histopathology that occurs in the kidneys which shows the presence of necrosis, inflammatory cell infiltration, edema, fatty degeneration hemorrhage and melanomacrophages and the level of damage to the tissue depends on the concentration of metal contaminated in the fish organs. Damages that occur allegedly due to exposure of heavy metals that are dissolved in the waters of the fish ecosystem that has passed the threshold. Keywords: histopathology, kidney, Lake Tempe, lead metal (Pb), tilapia fish Share Link | Plain Format | Corresponding Author (A Nur Indri Paramita)

22	Marine Biology and Marine Disease	ABS-34
IDENTIFICATION OF Aeromonas hydrophila BACTERIA AND ITS EFFECT ON GILL HISTOLOGY IN LELE DUMBO (Clarias gariepinus) A. Ananda Sekar Ayu Pertiwi Syakir, A. Magfira Apada, and Dwi Kesuma Sari Hasanuddin University Abstract African catfish (Clarias gariepinus) is one of the most economically important fish species for successful aquaculture. Today the needs of African catfish increase, along with increasing population growth and demand for this catfish. The purpose of this study was to determine the histological features of gills in African catfish that were positively infected by Aeromonas hydrophila. The samples used were 5 African catfish. Sampling was done selectively on fish that showed symptoms of Aeromonas infection that were symptoms of sores such as ulcers on the skin, reddish sores on the mouth, protruding eyes and swollen abdomen. Identification of A. hydrophila bacterium conducted at the Microbiology Laboratory of Makassar City Health Office using a compact vitek-2 machine found that 2 samples of African catfish that were infected with A. hydrophila. Histopathological examination was carried out by gill organ preparations in fixation using 10% neutral buffered formalin (NBF), dehydration using series of alcohol dilution, embedding using paraffin, cutting with a thickness of 4 &#956-m and stained using haematoxylin eosin then observed. Damage or histopathology that occurs in the gills due to bacterial infection A. hydrophila namely fat degeneration, necrosis, hemorrhage and inflammatory cell infiltration. Keywords: Aeromonas hydrophila, african catfish, gills, histology Share Link | Plain Format | Corresponding Author (A. Ananda Sekar Ayu Pertiwi Syakir)

23	Marine Biology and Marine Disease	ABS-36
Histopathology Overview of Tilapia (Oreochromis Mossambicus) Liver Organs Contaminated by Lead Metal (Pb) in Lake Tempe, Wajo Regency Riri Apriani Jabbar (a*), Dwi Kesuma Sari (b), Akbar Tahir (c) (a) Hasanuddin University Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, Telp. (0411) 586010 Psw. 1. Fax. (0411) 586297. (b) Faculty of Medicine, Majoring in Veterinary Medicine. Abstract Tilapia fish (Oreochromis Mossambicus) is one of the most popular freshwater fish in the community because it is easy to breed and its survival is high and group. The purpose of this study was to determine the histopathology of the liver in tilapia fish contaminated with lead heavy metal (Pb) in Lake Tempe,Wajo Regency. This research was conducted in March to June 2020. The number of fish samples used in this study was 21 fish. Measurement of lead heavy metal content (Pb) contained in the liver of tilapia fish (Oreochromis mossambicus). Measurement of heavy metal content was carried out with Atomic Absorption Spectrphotometer and obtained lead metal content (Pb) 0.14-2.8 microgram per gram with an average of 0.82 plus minus 0.83 microgram per gram. Preparation of liver organ fixation using 10 percen neutral buffered formalin (NFB), multilevel alcohol is used for dehydration, embedding using parafifin and haematoxylin-eosin staining. Analysis of the data used is descriptive qualitative. This research shows that lead metal (Pb) causes damage to the liver of tilapia fish. Damage to the liver in the form of inflammatory cell infiltration, fat degeneration, hemorrhage and necrosis. Damage that occurred as a result exposure to heavy metals, dissolved in the aquatic ecosystem that passed the threshold limit Keywords: histopathology, lake tempe, liver, lead metal, tilapia fish Share Link | Plain Format | Corresponding Author (Riri Apriani Jabbar)

24	Marine Biology and Marine Disease	ABS-39
Identification of Aeromonas hydrophila Bacteria and its Effect to Histopathology of African Catfish (Clarias gariepinus) kidney Ayu An Nisaa, A. Magfira Satya Apada, Dwi Kesuma Sari Universitas Hasanuddin Abstract African Catfish is one of fresh water comodity that liked by many people so the african catfish demand is pretty high. The purpose of this study is to find out histopathology change of african catfish kidney that infected by Aeromonas hydrophila. Samples were used is 5 african catfish. Samples taken were catfish which showed clinical symptoms in the form of lesions on the surface of the skin, swollen abdomen and pale skin color. Aeromonas hydrophila identification was done by biochemical test using Vitek 2 Compact machine and the result is two out of five sample are positive infected by Aeromonas hydrophila. Preparations of kidney were fixed using 10% neutral buffered formalin (NBF), dehydration using graded alcohol, embedding using paraffin, cutting with a thickness of 5 &#956-m and stained with haematoxilin eosin and then observed. Analysis of the data used is descriptive qualitative. Based on observations obtained by damage or histopathology that occurs in the kidneys namely fat degeneration, connective tissue formation, necrosis, hemorrhage, infiltration of inflammatory cell and an increase in the number of melanomacrophages. Changes in histological structure that occur is a reaction from the body of catfish infected with Aeromonas hydrophila bacteria. Keywords: Aeromonas hydrophila, African catfish, histopathology, kidney. Share Link | Plain Format | Corresponding Author (Ayu An Nisaa)

25	Marine Biology and Marine Disease	ABS-41
Identification of Aeromonas hydrophila Bacteria and its Effect to Histopathology of African Catfish (Clarias gariepinus) Liver A. Regita Dwi Cahyani, A. Magfira Satya Apada, Dwi Kesuma Sari Hasanuddin university Abstract African Catfish (Clarias gariepinus) is one of fresh water comodity that like by many people because it is easy to breed and has economic value. The purpose of this study was to find out histopathology change of african catfish liver that infected by Aeromonas hydrophila. This research was conducted in May to June 2020. The number of fish samples used in this study was 5 fish. Samples taken were catfish which showed clinical symptoms in the form of lesions on the surface of the skin, swollen abdomen and pale skin color. Identification of A. hydrophila was done by biochemical test using Vitek 2 Compact machine. The result of this test is two out of five sampel are positive infected by A. hydrophila. Histopathological examination was carried out by fixing the liver using 10% neutral buffered formalin (NFB), multilevel alcohol is used for dehydration, embedding using parafifin, cutting and haematoxylin-eosin staining. Analysis of the data used is descriptive qualitative. This research shows the damage or histopathology that occurs in the liver. Damage to the liver in the form of fat degeneration, inflammatory cell infiltration, hemorrhage and necrosis. This damage occurs as a result of the body of catfish infected with A. hydrophila bacteria. Keywords: Aeromonas hydrophila, African catfish, liver, histopathology Share Link | Plain Format | Corresponding Author (A. Regita dwi Cahyani)

26	Marine Biology and Marine Disease	ABS-44
Prevalence of Argulus indicus on wild fish in Towuti lake Amriana1, Dwi Kesuma Sari2, Hilal Anshary1 1Department of Fisheries, Faculty of Marine science and Fisheries, Universitas Hasanuddin, Makassar, Indonesia. 2Department of Veterinary Medicine, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia. Abstract Branchiurans are cosmopolitan ectoparasites, that has been infecting freshwater, brackish and marine fish. They attach wide range hosts and have transmitted in various regions of the world. The study aim to determine the prevalence of Argulus indicus on freshwater fish in Towuti lake, South Sulawesi, Indonesia. A total of 102 Anabas testudineus, 74 Cichlasoma trimaculatum, 84 Oreochromis niloticus, and 113 Channa striata were caught from Towuti lake from February to May in 2019 using fish pot and trammel net. Argulus were obtained from the head, fins, and surface body by visual observations. They were preserved in 70% alcohol and cleared in 10% KOH. Argulus indicus was identified by morphological and genetic analysis. Prevalence and main intensity of Argulus indicus was highest in Channa striata 81% and 5.71 ind/fish, respectively. In contrast the lowest infection in Cichlasoma trimaculatum was 4.1% and 1.66 ind/fish, respectively. Keywords: Ectoparasite, infection, fish lice, Freshwater, Diseases Share Link | Plain Format | Corresponding Author (Amriana amriana)

27	Marine Biology and Marine Disease	ABS-50
Isolation and characterisation of Edwardsiella tarda in barred bichir fish (Polypterus delhezi, Boulenger 1899) with septicemia: A first case report Rifky Rizkiantino (1), I Wayan Teguh Wibawan (1*), Fachriyan Hasmi Pasaribu (1), Retno Damajanti Soejoedono (1), Wyanda Arnafia (2), Vengine Ulyama (2), Kenda Adhitya Nugraha (3) 1) Division of Medical Microbiology, Department of Animal Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, IPB University, Raya Dramaga Rd., Bogor, Indonesia 2) Research and Development Division, Tekad Mandiri Citra, Inc., Mekar Raya Rd. Kav. 9, Panyileukan, Bandung, Indonesia 3) Aquatic Veterinarian Practitioner, Indo Fish-Vet: Mobile Fish Clinic and Aquatic Laboratory Services, Jakarta, Indonesia Abstract A barred bichir fish (Polypterus delhezi) was found dead at the bottom of the aquarium owned by a client. Results of anatomical pathology examination indicated pathology lesions in the form of the opacity of the eye (cloudy eye), hemorrhagic in the muscles, and discoloration of the liver (yellowish). Bacteriological examination was performed. The results of the morphological, biochemical, and molecular examination utilizing the 16S rRNA gene and sequencing analysis of the PCR product indicated that the isolate from liver organ was identified as Edwardsiella tarda bacteria. Antibiotic susceptibility test showed that the isolate was resistant to tetracycline and oxytetracycline and intermediate to erythromycin. Based on the results of the bacterial examination and Koch^s postulate, it was concluded that the fish was dead due to the condition of septicemia from Edwardsiella tarda infection. The result of this study serves as the first case report in the world and in Indonesia for the occurrence of edwardsiellosis in barred bichir fish. Keywords: barred bichir fish-edwarsiellosis-Edwardsiella tarda-Polypterus delhezi-septicemia Share Link | Plain Format | Corresponding Author (Rifky Rizkiantino)

28	Marine Biology and Marine Disease	ABS-51
Marine White Spot Disease on Stonefish Synanceia at Jakarta Aquarium Indonesia ZB Gandong, A Ris Hasanuddin University, Veterinary Medicine Study Program, Makassar Abstract White spot disease or Cryptocaryonosis could be found on freshwater or saltwater fish. This infection caused by ciliated protozoa called Cryptocaryon irritans in saltwater fish, which has a round-shape and dark-colour. Cryptocaryonosis could cause economic losses due its long treatment. Further, the disease has been reported happening in Indonesia but never documented in conservation area. Cryptocaryonosis was found in one of conservation areas namely Jakarta Aquarium Indonesia especially in stonefish Synanceia. General examination was done before going further to microscopic test with the chosen sample of skin scrape. The result of the examination was there are ciliated protozoa with dark-colour and round-shape. Copper treatment and fresh water dip was done by the veterinarians to treated the disease. A few days later, the infestation of C. irritans was slightly reduced. All in all, copper treatment and fresh water dip shows positive effects to treat C. irritans on stonefish Synanceia in Jakarta Aquarium Indonesia. Keywords: White Spot Disease, Stonefish Synanceia, Jakarta Aquarium Indonesia, Conservation Area, Microscopic Examinations Share Link | Plain Format | Corresponding Author (Zulfikar Basrul)

29	Marine Biology and Marine Disease	ABS-60
Histopathological View of Silver Barb (Barbonymus gonionotus) Kidney that Contaminated by Heavy Metal Cadmium (Cd) in Tempe Lake, Wajo Distric Achmad Yusril Ihzamahendra, Dwi Kesuma Sari, Abdul Wahid Jamaluddin Kedokteran Hewan Fakultas Kedokteran Universitas Hasanuddin Abstract Silver barb (Barbonymus gonionotus) is widely consumed by the community and is the dominant type of fish caught throughout the year in Lake Tempe. The purpose of this study was to determine the histopathological view of the kidneys in silver barb contaminated with heavy metal cadmium (Cd). Samples were used is 15 silver barb with each of 5 kidney samples at each station. Measurement of heavy metal content was carried out with an Atomic Absorption Spectrophotometer (AAS) and obtained cadmium (Cd) concentrations in the kidney organs of contaminated fish which were 0.29 until 1.94 mikrogram / g with an average of 0.77 plus minus 0.12 mikrogram / g. Kidney organ preparations were fixed using 10 percent neutral buffered formalin (NBF), dehydration using graded alcohol, embedding using paraffin, cutting with a thickness of 5 micron and stained with haematoxilin eosin and then observed. Analysis of the data used is descriptive qualitative. Based on observations obtained by damage or histopathology that occurs in the kidneys namely fat degeneration, connective tissue formation, necrosis, hemorrhage and an increase in the number of melanomacrophages. The level of damage to the tissue depends on the concentration of the metal contaminated in the fish^s organs. Damages that occur are thought to be caused by exposure to heavy metals dissolved in the waters of the fish ecosystem which have passed the threshold. Keywords: cadmium, histopathology, kidney, silver barb, tempe lake Share Link | Plain Format | Corresponding Author (Achmad Yusril Ihzamahendra)

30	Marine Biology and Marine Disease	ABS-61
Effect of Cadmium (Cd) Heavy Metal Contamination in Tempe Lake, Wajo Distric on Histopathology of Silver Barb Liver (Barbonymus gonionotus) Dwi Ainun Utari, Dwi Kesuma Sari, Abdul Wahid Jamaluddin Kedokteran Hewan Fakultas Kedokteran Universitas Hasanuddin Abstract Silver barb (Barbonymus gonionotus) is one of the natural wealth of Indonesian waters classified as fish that are easily adaptable and widely consumed by people.The purpose of this study was to determine the effect of Cadmium (Cd) Heavy Metal Contamination in Tempe lake, Wajo Distric on Histopathology of Liver in Silver barb (Barbonymus gonionotus). The samples used were fifteen Tawes with each of the five Tawes liver samples at each station. Measurement of heavy metal content is done with Atomic Absorption Spectrofotometi (AAS) and obtained cadmium (Cd) concentrations in the liver organs of contaminated fish which were 0,04 until 0,33 mikrogram/g with an average of 0.11 plus minus 0.08 mikrogram/g. Liver organ preparations in fixation using neutral buffered formalin (NBF) 10 percent, dehydration using multilevel alcohol, embedding using paraffin, cutting with a thickness of 4 micron stained using haematoxilin eosin was then observed. Analysis of the data used is descriptive qualitative. Based on the results of observations obtained damage or histopathology that occurs in the liver that shows the existence of degeneration, hemorrhage, infiltration of inflammatory cells and necrosis. The level of damage to the tissue depends on the concentration of the metal contaminated in the fish^s organs. Most of the damage that occurred was thought to be due to exposure to heavy metals dissolved in the waters of the fish ecosystem which had passed the threshold. Keywords: Cadmium, histopathology, liver, silver barb, tempe lake Share Link | Plain Format | Corresponding Author (Dwi Ainun Utari)

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