UVB radiation induces skin inflammation and changes in bone marrow cell population on rats Verda Farida (a), Dion Adiriesta Dewananda (b), Muhammad Novrizal Abdi Sahid (c), Siti Isrina Oktavia Salasia (b*)
a) Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Ahmad Dahlan, Jl. Prof. Dr. Soepomo, S.H., Yogyakarta 55164, Indonesia
b) Department of Clinical Pathology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl. Fauna 2, Karangmalang, Yogyakarta 55281, Indonesia
*isrinasalasia[at]ugm.ac.id
c) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jl. Sekip Utara, Yogyakarta 55281, Indonesia
Abstract
Ultraviolet B (UVB) radiation is commonly used to study skin-related inflammation mediated by skin-resident immune cells. These cells are mobile and can release various pro-inflammatory proteins that might affect blood cells that not reside in the skin. This study aimed to investigate the skin condition and global blood cell condition after UVB radiation. Ten rats were divided equally into two groups, where the first group (K1) as control and the second group (K2) was exposed to UVB within 30 minutes/day for consecutive nine days. Prior to radiation, the rats were shaved at the back with size 2 x 2 cm. After the final UVB radiation blood samples from bone marrow were collected, proceed for Giemsa staining. The skin tissues were processed for Hematoxylin and Eosin, Tuloidin blue, and Mallory staining. Cornification, pigmentation, collagen-density depletion, haemorrhage were only observed in the skin tissues of group with UVB radiation. The erythroid and myeloid ratio was increased to around 1.6:11.2 from the normal ratio of 1:4. In addition, the leukocytes number is increased from 8.37x103 cells/mm3 (normal group) to 14.396x103 cells/mm3. These results show that UVB radiation affected not only skin-resident cells but also blood cells.
