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91	Zoonotic Disease and Tropical Disease	ABS-84
Exploration of gram - negative bacterial contamination edible bird nest in bone district Dhiya Nabilah Jafar, Baso yusuf, A. Magfirah Satya Apada, Rizalinda Sjahril, Sitti Arifah Hasanuddin University Abstract Edible nest swiftlet (Aerodramus fuciphagus) is a type of bird that produces high economic nest that made from its saliva. The nest has many benefits and a very delicious taste, but the edible bird nest which is a food product of animal orginin has a risk of harmful microbial contamination for human health. This study aims to determine the presence of bacterial contamination found in walet nests and determine the types of bacteria found in it. In this study the sample used was taken from walet bird houses in 5 different regions in Bone. Bacterial identification was carried out at the Microbiology Laboratory of Makassar City Health Office. Sample from each region were taken as much 5,6 gram and then dissolved into 50 ml of the fertilizer solution Brain-heart Infusion Broth (BHIB). Then the colonies that had grown in Brain-heart Infusion Broth BHIB were inoculated on Mac Conkey agar media. After that, Colonies on Mac Conkey is taken and put into a tube containing 3 ml of suspense and then homogenized. Then the turbidity test was carried out using Densicheck until it reached 0.5 Mcfarland and put into Vitek 2 compact system. The results showed that samples from rural areas identified Pseudomonas aeruginosa. The samples from the urban areas identified Proteus mirabilis. Samples from mountainous areas identified Escherichia coli. Samples from marine areas identified Enterobacter aeruginosa. And samples from rice fields identified Enterobacter cloaca complex. Keywords: Bacteria, Vitek 2 compact, edible bird nest Share Link | Plain Format | Corresponding Author (Dhiya Nabilah Jafar)

92	Zoonotic Disease and Tropical Disease	ABS-95
Malaria vaccination targeting parasite stages in mosquitoes to block transmission Yenni Yusuf (a) a) Department of Parasitology, Faculty of Medicine, Hasanuddin University Makassar Abstract Transmission of malaria involves Anopheles mosquitoes as the vectors of Plasmodium parasite. Malaria eradication includes the distribution of insecticide-treated bednets. However, the Anopheles mosquitoes have developed insecticide resistance hence it is necessary to find other modalities to eradicate the disease. Because the parasites undergo several stages in the mosquito midgut before developing into infective stages that migrate to the salivary gland, it is of interest to target the mosquito stages in a vaccination to block the transmission. Human vaccination with the antigen of the mosquito stages may induce production of specific antibodies against the stages that might be transferred into mosquito during blood meal that will bind with the antigen of the mosquito stages in the midgut and consequently inhibit the development of the parasite. Such vaccine is called transmission blocking vaccine (TBV). An effective TBV should induce high titer of antibody for a long time. One of the leading antigen is Pfs25. Here we review the update of TBV development targeting Pfs25, the challenges, and the alternative solution of the challenges. Keywords: TBV, Pfs25, malaria, Plasmodium, mosquito stages Share Link | Plain Format | Corresponding Author (Yenni Yusuf)

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