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31	Marine Biology and Marine Disease	ABS-81
Histology of Medaka Sulawesi (Oryzias Celebensis) Fish Skin while Process of Puncture Wound Healing Kadek Dian Krisna Putri K,Dwi Kesuma Sari, and Irma Andriani Hasanuddin University Abstract Sulawesi Medaka fish (Oryzias celebensis) should be developed as animal models for additional information about Medaka Sulawesi because this fish is the endemic fish of Sulawesi. This study aims to observe the skin histology of the Sulawesi medaka fish during the healing process of the puncture wound. This study used the Sulawesi medaka fish which were divided into 3 groups, K1 as a control group without treatment as many as 6 fish, K2, the puncture wound treatment group of 6 fish and K3, the group of fish that were given puncture wounds then kept in the water which had been given with methylene blue. The wound healing process of K2 and K3 then observe on days 1, 3, 5, and 7. The result shows that the redness around the wound and the size of the wound decreases every day both on K2 and K3. The observations on 1st day of K2 and K3 showed that there were much inflammatory cells, fibroblasts and collagen fibers are found in few and granulation tissue were not found, while on the 3rd day there were still much inflammatory cells, fibroblasts and collagen fibers in more ways than 1st day and granulation tissue was still not found. Histological observations on 5th day of K2 showed that there were more fibroblasts, inflammatory cells were decreased, and a large amount of granulation tissue was found, while on 7th day of K2, fibroblasts were found but not as much as 3rd and 5th day with high density, inflammatory cells were also still visible, more collagen fibers were found on K2 and K3. Keywords: histology, skin, medaka, methylene blue, Oryzias celebensis, wound Share Link | Plain Format | Corresponding Author (Kadek Dian Krisna Putri K)

32	Marine Biology and Marine Disease	ABS-96
Identification of Tissue Changes in Shrimp Vaname (Penaeus vannamei) Infected with White Spot Syndrome Virus A. Fadlillah Nal Azkina, Hilal Anshari, Dwi Kesuma Sari, A. Magfira Satya Apada Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, South Sulawesi, 90245, Indonesia Abstract Vaname shrimp is one of the shrimp that has economic value and is an alternative type of shrimp that can be cultivated in Indonesia, besides tiger shrimp (Panaeus monodon) and white shrimp (Panaeus merguensis). The purpose of this study was to determine the histological picture of tissue changes that occur in shrimp vaname (Penaeus vannamei). The samples used were vaname shrimp collected and then prepared, afterwards the samples were extracted using DTAB-CTAB, then continued amplified using conventional PCR machines, where the first step PCR was first then Nested PCR was added. Samples that had been amplified were then electrophoresis with a voltage of 150V for 30 minutes then read results. Gills and hepatopancreas samples were then taken for histological preparations. Preparation is done by method with a sample processed in tissue processing then embed and blocked with paraffin then given hematoxylin eosin (HE) staining and analyzed descriptively qualitatively. PCR test results obtained different results in each organ, as shown in the swimming leg organ with the appearance of 3 (three) DNA bands / bands parallel to the marker (910 bp, 550 bp and 296 bp), which means the swimming leg organ is infected with WSSV the level of severe (severe) with WSSV virus infection is 2000 copies / reaction, whereas in the gill organs the emergence of DNA bands / bands are only 2 (two) bands parallel to the 296 bp and 550 bp markers indicating that the organ is infected with the WSSV virus at medium level with WSSV virus infection is 200 copies / reactions and hepatopancreas organs that show negative results with only the appearance of a smear. Histology test results showed a tissue change in the form of inclusion body and necrosis of the gills and hepatopancreas. Keywords: Histology, PCR, Vaname Shrimp, White Spot Syndrome Virus (WSSV) Share Link | Plain Format | Corresponding Author (A Fadlillah Nal Azkina)

33	One Health	ABS-15
Hormonal Induction of Gonad Maturation in Lemeduk fish (Barbonymus schwanenfeldii) using spawnprim Amalia Sutriana, Ahmad Baihaqi, Iwan Hasri, Juli Melia, Muhammad Hanafiah, Syafruddin, Nurliana Faculty of Veterinary Medicine, Universitas Syiah Kuala Abstract The purpose of this study was to analyze the effect of spawnprim hormone induction with different doses on gonadal maturity of lemeduk fish broodstock (Barbonymus schwanenfeldii). A completely randomized design (CRD) was used in this study with 4 treatments and 3 replications. Fish broodstock in group A (control) was not given any treatment, while fish broodstocks in groups B, C, and D were injected with spawnprim hormone at doses of 0.3 ml/kg body weight, 0.6 ml/kg body weight, dan 0.9 ml/kg body weight, respectively. The measured parameters were gonadal maturity level, broodstock weight gain, egg diameter, and fecundity. Data were analysed using one way analysis of variance (ANOVA). The result showed that the gonadal maturity level (GML) in groups B, C, and D (GML III and IV) was better than group A (GML I). Statistical analysis showed that the induction of spawnprim hormone on lemeduk broodstocks significantly affect (P<0,05) broodstock weight gain, egg diameter, and fecundity. The optimal dose of spawnprim hormon for lemeduk was 0,6 ml/kg body weight which recorded the average broodstock weight gain, egg diameter, and fecundity were 0.011 kg, 1.55 mm, and 102.15 eggs, respectively. Keywords: lemeduk fish, gonadal maturity, spawnprim Share Link | Plain Format | Corresponding Author (Amalia Sutriana)

34	One Health	ABS-29
Assesment of Heavy Metal Pollution in Lung and Kidney of Broiler and Its Correlation of Water and Animals Feed Amelia Ramadhani Anshar, Hera Maheshwari, Huda Shalahudin Darusman Universitas Hasanuddin, Institut Pertanian Bogor Abstract Cadmium belongs to heavy metal with high anthropogenic pollutant and non-essential to physiological function. Cadmium present in soil, water, air as well as in food. Reguler intake of consuming cadmium leads to physiological disorders in poultry animal as well as humans as one who consume it. The aim of this study was to analyze the profile of cadmium content in environtmental samples (poultry feed and water) and it correlated with the degree of contamination in broiler tissue. The study used 30 sample of poultry feeds and animal water, 30 sample of chicken lungs and 30 sample of chicken kidneys. Feed samples were taken at each floc where the chickne were previously taken. The results showed level of cadmium in feed between 0.009-0.202 mg / kg and 0.0068-0.0096 mg / l of cadmium in the water. Cadmium in the lungs and kidneys wass positively contaminated by cadmium with a range of 0.438-0.655 mg / kg and it was significantly different (p <0.05) among the organs. A strong positive correlation was found between cadmium and water levels in kidneys (r = 0.925). In contrast, a weak positive correlation (r = 0.624) was found in the relationship between cadmium in feed and lung. As the conclusion, cadmium consentration in the poultry feed and water increased significantly to the concentration of cadmium deposited in organs. Keywords: Broiler, Cadmium, Lung, Kidney Share Link | Plain Format | Corresponding Author (AMELIA RAMADHANI ANSHAR)

35	One Health	ABS-42
Antibiotic susceptibility of lactic acid bacteria isolated from duck and Jawa super chicken excreta intended for use as probiotic Dini Dwi Ludfiani (1), Widya Asmara (2*), Agnesia Endang Tri Hastuti Wahyuni (2), and Pudji Astuti (3) 1) Student in Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna Number 2, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia 55281 2) Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna Number 2, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia 3) Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna Number 2, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia *) Corresponding author: wied_as[at]ugm.ac.id Abstract Lactic acid bacteria (LAB) have many reported to be probiotics for animals and humans. But to assure their safety in feed or food, antibiotic susceptibility test is necessary as a precautionary measure that should be taken to protect animals, humans, and the environment from potentially unsafe microorganisms (cause resistance). The aim of this study was to determine the antibiotic susceptibility of LAB strains that isolated from duck and Jawa super chicken excreta that were collected from Sleman, Yogyakarta. The LAB isolates were identified phenotypically based on morphology, physiology, and biochemical characteristics. Antibiotic susceptibility test using the disc diffusion method. Five antibiotics were commonly used for the treatment of infections in animals and humans, which were ampicillin (10 ug), bacitracin (10 ug), erythromycin (15 ug), streptomycin (10 ug), and tetracycline (30 ug). The results showed that all isolates were identified as Lactobacillus spp. (41.67% L. brevis and 58.33% L. pentosus). All isolates showed different resistance to antibiotics. A total of 91.67% LAB isolates were resistant to streptomycin and 4 isolates were found resistant to all antibiotics. One LAB isolate from duck excreta was found sensitive to three antibiotics (ampicillin, erythromycin, and tetracycline) and it could possibly be used for probiotic. Keywords: Antibiotic resistance, lactic acid bacteria, duck excreta, Jawa super excreta Share Link | Plain Format | Corresponding Author (Dini Dwi Ludfiani)

36	Veterinary Anatomy, Physiology, Pharmacology	ABS-8
Effect of Garcinia atroviridis and Kunci Pepet Extracts on the Recovery Time of Fatigue in Obese Rats Min Rahminiwati (a), Panji Andhika Maharta (a), Pudji Achmadi (b), Sidik Muhamad Leluala (a), Aryani Sismin Satyaningtijas (b) a).Divison of Pharmacology and Toxicology, Departement of Anatomy, Physiology and Pharmacology, Fakulty of veterinary Medicine IPB University (Bogor Agricultural university), Bogor Indondesia b).Divison of Physiology , Departement of Anatomy, Physiology and Pharmacology, Fakulty of veterinary Medicine IPB University (Bogor Agricultural university), Bogor Indondesia Abstract The study was conducted to determine the effect of combined Garcinia atroviridis and Kunci Pepet (Kaempferia rotunda) on the stamina of obese rats (Rattus norvegicus) induced by a high fat diet. The amount 20 of obese Male Spragua dawley Rats were given combined extract of Garcinia and Kaempeferia dose 32 mg/200 g b.w. (DI), 96 mg/200 g b.w. (DII) and 288 mg/200g b.w. (DIII) respectively and aquadest as negative control. Forced swimming test was done to determine stamina by determining the duration of rat swimming time and blood lactate level. Blood lactate level was measured by Roche Accutrend Plus Meter Kit four times before and after the first forced swimming test (FST) and after getting rest immediately prior to and after the second FST. Combined Garcinia and Kunci Pepet was administered once a day for 30 consecutive days prior to and once a day after the first FST. The obtained data were analyzed statistically by one-way Analysis of Variance (ANOVA) method followed by the Duncan test. The results showed that the combined Garcinia atroviridis and Kunci pepet at a dose of 288 mg/200 g b.w. increase the duration of swimming time and blood lactate level in the first FST, as well as accelerate a decrease in blood lactate level after resting time. Booster stamina by readministration of combined Garcinia and Kunci Pepet before second FST to this group leads to the duration of second FST and blood lactate level insignificantly different from those of control. Combined Garcinia atroviridis and Kunci Pepet was capable to increase stamina in a continues period of activity but not in repeated activity separated by resting time. It was concluded that the combined Garcinia-kunci pepet increases stamina and accelerates the recovery of fatigue but does not boost the stamina in the presence of fatigue and fasting. Keywords: Kunci Pepet, Garcinia atroviridis, stamina, blood lactate Share Link | Plain Format | Corresponding Author (Min Rahminiwati)

37	Veterinary Anatomy, Physiology, Pharmacology	ABS-19
Anthelmintic Activity of Jatropha (Jatropha curcas linn) Leaf Extract Against Ascaridia galli Worms In Vitro Hasri Ainun, Adryani Ris, Abdul Wahid Jamaluddin Universitas Hasanuddin Abstract Ascariasis is an intestinal worm disease caused by Ascaridia galli. This disease can attack the small intestine and cause a decrease in productivity of local breed chickens. Worm infections in chickens can be suppressed by taking precautions and treatment measures such as improving the management of the cage and providing anthelmintic. Medicinal plants that can be used as an alternative anthelmintic are jatropha leaves (Jatropha curcas linn) which contain secondary metabolites such as alkaloids, saponins, tannins, phenolics, flavonoids, triterpenoids, steroids, and glycosides. The study aims to determine the anthelmintic activity of Jatropha leaf extract against Ascaridia galli worms. 75 samples of Ascaridia galli worms were taken from chicken intestines in traditional markets. This research was conducted in vitro with five treatments consisting of one positive control group using Levamisole, one negative control group with 0.5% NaCMC, and three groups of Jatropha leaf extract (EDJ) with concentrations of 10%, 15% and 20 %. The results of the study showed that jatropha leaf extract at a concentration of 20% gave a better anthelmintic effect compared with administration of Levamisole as a positive control (+) and jatropha leaf extract had LC50 and LT50 at concentrations of 5.49% and 96.006 minutes. Keywords: Ascaridia galli, anthelmintic, jatropha leaves, LC50, LT50 Share Link | Plain Format | Corresponding Author (Hasri Ainun)

38	Veterinary Anatomy, Physiology, Pharmacology	ABS-25
Activity Test of binahong Leaves (Anredera Cordifolia Ten. Steenis) to Ascaridia galli Worms In vitro Kasriana Nurasmi (a), Zainal Abidin Kholilullah (a), Abdul Wahid Jamaluddin (a*) a) Faculty of Medicine, Department of Veterinary Medicine, Hasanuddin University, Jalan Perintis Kemerdekaan KM.10, Tamalanrea Indah,Tamalanrea, Makassar City *abdulwahidjamaluddin[at]unhas.ac.id Abstract Parasitic disease in chickens that is often encountered is Ascaridiasis. The disease is caused by Ascaridia galli worms that attack the intestines. These parasites cause losses in the form of weight loss and growth retardation, decreased egg production and decreased egg quality. Medicinal plants that can be used as an alternative to anthelmintics are binahong leaves (Anredera Cordifolia Ten. Steenis) containing saponins, flavonoids, tannins which are known to have anthelmintic effects. This study aims to determine the anthelmintic activity of binahong leaf extract against Ascaridia galli worms. This research is an experimental laboratory. This research was conducted in vitro with the research subject being Ascaridia galli worms that are actively moving. Subjects were divided into 5 groups, each group consisting of 5 worms. The positive control group used Levamisole, the negative control group used 0.5% Na CMC, while the treatment group consisted of binahong leaf extract (Anredera Cordifolia Ten. Steenis) with concentrations of 10%, 15% and 20%. The results of the study showed that binahong leaf extract starting from a concentration of 20% gave an anthelmintic effect. Keywords: anthelmintic, binahong leaves, Ascaridia galli worms. Share Link | Plain Format | Corresponding Author (Kasriana Nurasmi)

39	Veterinary Anatomy, Physiology, Pharmacology	ABS-40
Potential of Indonesian Bay Leaf (Syzygium polyanthum) as Anthelmintic Against Ascaridia galli by Scanning Electron Microscope Santika Anggrahini(a), Irkham Widiyono(b*), Soedarmanto Indarjulianto(b), Joko Prastowo(c) a) Student of Doctoral Program Veterinery Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Bulaksumur, Sleman, Yogyakarta, 55281, Indonesia b) Department of Veterinary Interna Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada, 55281 Bulaksumur Sleman, Yogyakarta, 55281, Indonesia *Irkhamwidiyono[at]ugm.ac.id c) Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, 55281 Bulaksumur Sleman, Yogyakarta, 55281, Indonesia. Abstract Anthelmintic resistance has emerged as a major problem in parasite control, hence, it is necessary to explore the possibility of using herbal plants as an alternative to anthelmintics. This study aims to determine the potential of Indonesian bay leaves that are extracted using ethanol as an anthelmintic alternative against Ascaridia galli by employing in vitro method. Phytochemical method was used to indicate whether the extract contained the chemical being observed. In vitro method was employed to determine the effect of Indonesian bay leaf extract (BLE). Indicator was based on the resistance of worm movement which was then evaluated ultra-structurally using Scanning Electron Microscope. This study was conducted to 4 experimental groups, consisting of 3 BLE treatment groups with varying degrees of solution concentrations (2%, 8% and 14%) and a negative control group. The results showed that A. galli in all groups did not die during the 10 hours immersion. BLE up to a concentration of 14% can damage the cuticle of A. galli. Based on this experiment, it was concluded that BLE is potential to be an anthelmentic against A. galli. Further research is then need to be conducted to determine the ideal dosage. Keywords: Syzygium polyanthum, Ascaridia galli, in vitro, SEM Share Link | Plain Format | Corresponding Author (Santika Anggrahini)

40	Veterinary Anatomy, Physiology, Pharmacology	ABS-47
Anthelmintic Activity of Jatropha (Jatropha curcas linn) Leaf Extract Against Ascaridia galli Worms In Vitro Hasri Ainun1, Adryani Ris1,Muh. Nur Amir2, Abdul Wahid Jamaluddin1 1Veterinary Study Program, Faculty of Medicine, Universitas Hasanuddin 2Faculty of Pharmacy, Universitas Hasanuddin Abstract ABSTRACT Ascariasis is an intestinal worm disease caused by Ascaridia galli. This disease can attack the small intestine and cause a decrease in productivity of local breed chickens. Worm infections in chickens can be suppressed by taking precautions and treatment measures such as improving the management of the cage and providing anthelmintic. Medicinal plants that can be used as an alternative anthelmintic are jatropha leaves (Jatropha curcas linn) which contain secondary metabolites such as alkaloids, saponins, tannins, phenolics, flavonoids, triterpenoids, steroids, and glycosides. The study aims to determine the anthelmintic activity of Jatropha leaf extract against Ascaridia galli worms. 75 samples of Ascaridia galli worms were taken from chicken intestines in traditional markets. This research was conducted in vitro with five treatments consisting of one positive control group using Levamisole, one negative control group with 0.5% NaCMC, and three groups of Jatropha leaf extract (EDJ) with concentrations of 10%, 15% and 20 %. The results of the study showed that jatropha leaf extract at a concentration of 20% gave a better anthelmintic effect compared with administration of Levamisole as a positive control (+) and jatropha leaf extract had LC50 and LT50 at concentrations of 5.49% and 96.006 minutes. Keywords: Keywords: Ascaridia galli, anthelmintic, jatropha leaves, LC50, LT50 Share Link | Plain Format | Corresponding Author (Hasri Ainun)

41	Veterinary Anatomy, Physiology, Pharmacology	ABS-48
Activity Test of bitter melon Leaf Infusion (momordica charantia) as an anthelmintic toward Ascaridia galli worms in vitro Andi Muhammad Taufan1, Muh. Nur Amir2, Farida Nur Yuliati3, Adryani Ris1, Abdul Wahid Jamaluddin1 1Veterinary Study Program, Faculty of Medicine, Universitas Hasanuddin 2Faculty of Pharmacy, Universitas Hasanuddin 1Faculty of Husbandry, Universitas Hasanuddin Abstract ABSTRACT Ascaridia galli is a gastrointestinal parasite in poultry which can cause weight loss, slow growth and affect egg production. Chickens that are infected can cause damage to the integrity of the intestinal villi to severe inflammation of the intestinal mucosa. Bitter melon leaf (Momordica charantia) is known to have an anthelmintic effect which can be used as an alternative in the treatment of worms disease. The purpose of the research is to determine activity of bitter melon leaf infusion toward Ascaridia galli conducted in vitro. In the research, it uses 72 Ascaridia galli that divided into 4 groups consisting of positive control group (Levamisole), negative control group (Aquadest), and 2 treatment group with 10%, and 20% bitter melon leaf infusion concentration, respectively. Each group replicated three times. The worms were immersed in the test solution and observations were performed every 15 minutes and counted the number of worms that died. The results showed that pare leaf infusion with concentrations of 10% and 20% had an anthelmintic effect but was no better than levamisole as a positive control. Keywords: Ascaridia galli, anthelmintik, Bitter melon leaf Share Link | Plain Format | Corresponding Author (Hasri Ainun)

42	Veterinary Anatomy, Physiology, Pharmacology	ABS-49
Comparison of the Anthelmintic Power of Simplicia, Fresh and Dried Lamtoro Leaves (Leucanea leucocephala) Against Ascaridia galli worms. Muh Multazam B.H Abd.Hakim 1, Muh. Nur Amir2, Farida Nur Yuliati3, Adryani Ris1, Abdul Wahid Jamaluddin1 1Veterinary Study Program, Faculty of Medicine, Universitas Hasanuddin 2Faculty of Pharmacy, Universitas Hasanuddin 1Faculty of Husbandry, Universitas Hasanuddin Abstract ABSTRACT Lamtoro leaves can be used as an alternative to anthelmintic because they contain secondary metabolites such as alkaloids, tannins, flavonoids, triterpenoids and steroids. This study aims to determine the better anthelmintic effectiveness between the infusion of simplicia, fresh and dry leaves of lamtoro (Leucanea leucocephala) against Ascaridia galli. A total of 75 samples of Ascaridia galli worms were taken from the indicated gastrointestinal tract of chickens. In this study the samples were grouped into five treatments consisting of one positive control group, one negative control group, three groups of infusion, namely simplicia, fresh leaves and dried lamtoro. Each treatment group included five Ascaridia galli worms then replicated three times to maintain reliability. Observations were made every 15 minutes for 600 minutes to see the number of worm deaths. Simplicia infusion has the best worm-killing effectiveness with 100% presentation with a mean time of 295 minutes followed by fresh leaf infusion which kills worms with 100% presentation with an average time of 480 minutes and finally dry leaf infusion has the lowest worm-killing effectiveness with 100% presentation with a mean time of 595 minutes. The three treatment interventions were no better than the positive control which was able to kill worms with 100% presentation with a mean time of 115 minutes. Keywords: Keywords : Anthelmintic, Ascaridia galli, worms, Infusion, Lamtoro Share Link | Plain Format | Corresponding Author (Hasri Ainun)

43	Veterinary Anatomy, Physiology, Pharmacology	ABS-53
Evaluation of hematological and urine parameters of Anoa (Bubalus Spp.) at Environment and Forestry Research and Development Institute of Manado (BP2LHK) M. Zulfadillah Sinusi (a), Elphan Augusta Kajang (a), Diah Irawati Dwi Arini (b), Herry Sonjaya (c), Eddyman W. Ferial (d), Fika Yuliza Purba (a,e*) a) Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia. b) Environment and Forestry Research and Development Institute of Manado (Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan/BP2LHK), Manado, 95119, Indonesia. c) Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia. d) Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar, 90245, Indonesia. e) Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan. Abstract Anoa (Bubalus spp.) is an endemic species found only on Sulawesi island, Indonesia. The lack of intact review evaluating the physiological condition leads to difficulties in the conservation efforts of this endangered species. This study aimed to examine the hematological and urine parameters of Anoa at Environment and Forestry Research and Development Institute of Manado (BP2LHK). Four Anoa were used in this study, physiological conditions were recorded during the study period, while hematological and urine parameters were only examined once and twice, respectively, due to limited access to the animals. The level of hemoglobin was 17.18 g/dL, packed cell volume of 45.22 percent, total erythrocyte count was 10.46 x 106/microliter and total leukocyte count was 5.65 x 103/ microliter. The mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration was 43.38 fl, 16.48 gl, and 38.28 g/dL, respectively. The urine leukocytes were 0-15 leuko/ microliter, urobilinogen level was 3.50 micromol/L, protein value was 39.50 mg/dL, and urine pH and urine density were 9.00 and 1.00 g/mL, respectively. The urine sedimentation analysis showed the formation of the urinary crystal (amorphous crystal, calcium carbonate, and struvite). We conclude that hematological and urine parameters were not different among Anoa in this study. Keywords: Bubalus spp, hematology, urine analysis, Sulawesi Share Link | Plain Format | Corresponding Author (Elphan Augusta)

44	Veterinary Anatomy, Physiology, Pharmacology	ABS-59
Antibacterial activity of mangosteen skin extract (Garcinia mangostana L.) against Salmonella pullorum. Abdul Wahid Jamaluddin Corresponding author: abdulwahidjamaluddin[at]unhas.ac.id Abstract The content of alkaloids, flavonoids, phenols and tannins in the skin of the mangosteen fruit will make this plant suitable for testing as an active substance that can inhibit bacterial growth. Bacteria that often attack poultry include Salmonella pullorum. Salmonella pullorum is dangerous for ungags because this pathogenic bacteria can cause the death of broiler chickens by up to 80% by infecting the digestive tract of the chicken causing lime defecation. This study will test the activity of the skin extract for mangosteen against Salmonella pullorum bacteria using four types of concentrations, namely 40%, 20%, 10% to 5% were for negative control using 10% DMSO solution and for positive control using a disc antibiotic combination of Amoxicillin and clavulanate acid. 30. Muller Hinton Agar (MHA) media used for the bacterial inhibition test contained six treatment discs and was carried out triple. The results showed that Salmonella pullorum bacteria could be inhibited by the mangosteen rind extract even though using the smallest concentration in this study, which was 5% with a clear zone diameter of 11.2 mm. These results indicate that the growth of Salmonella pullorum can be inhibited using the mangosteen rind extract Keywords: Salmonella pullorum, antibacterial, mangosteen skin extract (Garcinia mangostana L.), in vitro Share Link | Plain Format | Corresponding Author (Abdul Wahid Jamaluddin)

45	Veterinary Anatomy, Physiology, Pharmacology	ABS-63
Obesity and ET-A Receptors expression on rat kidneys M M Syuaib(1,2*) U A Miskad (1) H Cangara (1) I Idris (3) R Masadah(1) A Zainuddin(4) H Dahlan(1) and T Fujimura (5) (1)Department of Pathology Anatomi, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia (2)Department of Physiology, Faculty of Medicine and Health, Alauddin State Islamic University, Makassar, Indonesia (3)Department of Physiology, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia (4)Department of Public Health, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia (5)Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan Abstract Endothelin system including Receptor Endothelin A (ET-A) is reported to play an important role in various diseases including obesity related chronic kidney disease (CKD). Several Journals have been studied about ET-A but assessment ET-A expression on tissue is less reported. ET-A predominate in vascular smooth muscle, their binding with ET-1 mediating many of the pathophysiological actions including vasoconstriction, reduction in renal blood flow and glomerulofiltration rate. We studied the differences of ET-A expressions in the various parts of cortex kidneys between case and control groups. The rats were divided into two groups based on the index Rohrer (IR). The case group (IR>30 gr/cm) was given a high-fat diet and the control (IR< 30 gr/cm) was assigned a standard diet. ET-A expressions was seen by Immunohistochemistry (IHC) from the paraffin-blocked kidneys. Result: In Cortex of Right Kidney, receptor ET-A of case group were more expressed than control group on various structures including glomerulus, distal tubule, artery, vena, and mesangial cell with p <0.05 but no differences were found between two group on proximal tubule structures. Keywords: Endothelin-A, obesity Share Link | Plain Format | Corresponding Author (Miswani Mukani Syuaib)

46	Veterinary Anatomy, Physiology, Pharmacology	ABS-78
The Alteration of Short-Time Incubation of Extreme pH Media to the Morphology of Freeze-Thawed Pasundan Bull Sperm Rini Widyastuti(a,b), Hera Maheswari (a), Cece Sumantri (c), Diah Nugrahani Pristihadi (a), Noer Muhamad Dliyaul Haq (a), Wahyudin(a), Arief Boediono(a*) (a) Departement of Anatomy, Physiology, and Pharmacology,Faculty of Veterinary Medicine, IPB University. Jl. Agatis Dramaga Bogor, West Java 16680, Indonesia *ab[at]apps.ipb.ac.id (b)Laboratory of Animal Reproduction and Artificial Insemination, Departement of Animal Production, Animal Husbandry Faculty,Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia (c) Department of Animal Production and Technology, Faculty of Animal Science, IPB University Abstract The present study aimed to evaluate the morphology of freeze thawed Pasundan bull sperm after short-time exposure to extreme acidic and alkali media. The freeze thawed Pasundan bull sperm were centrifuged for 500g for 5 minutes to remove the cryoprotectant. The sperm pellet was added with buffer media and aliquoted to 25 million sperm/ml. The extreme acidic and alkali media were prepared by addition of HCL or NaOH to buffer solution until reaches the pH 4 and 11. The buffer media was used as a control media. Each sample was incubated for 5 minutes with a specific pH media at room temperature, then add a buffered media until reach a pH 7. The evaluation of sperm morphology was performed after 15 minutes of incubation at room temperature. The results showed that the percentage of sperm with normal morphology significantly decreased after incubation at acidic and alkali media compared to control. Interestingly, the short time incubation in extreme acidic and alkali media significantly increased the percentage of sperm with major abnormal morphology, mainly abnormal head shape and abnormal acrosome compared to control. While the minor abnormal sperm defect was only affected by extreme acidic media. It can be concluded that the short period incubation in an extreme acidic or alkali media significantly induced a deleterious effect to morphology of freeze thawed Pasundan bull sperm although immediately returned to normal a physiologic condition. Keywords: Pasundan bull, freeze-thawed sperm, acidic, alkali, morphology Share Link | Plain Format | Corresponding Author (Rini Widyastuti)

47	Veterinary Anatomy, Physiology, Pharmacology	ABS-79
Potential of Cat (Felis Catus) Sperm Collected from Preserved Testes as a Model for Feline Karisma Mardatillah (a), Rini Widyastuti (a,b), Diah Nugrahani Pristihadi (a), Gunawan (c), Sigit Prastowo (d), Wahyudin (a), Boediono (a*) (a) Departement of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University, Jl. Agatis Dramaga Bogor, West Java, Indonesia 16680, Indonesia *ab[at]apps.ipb.ac.id (b)Laboratory of Animal Reproduction and Artificial Insemination,Departement of Animal Production, Animal Husbandry Faculty, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia (c) Department of Animal Production and Technology, Faculty of Animal Science,IPB University, Bogor, West java, Indonesia (d) Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Indonesia Abstract This research aimed was to determine the potential of spermatozoa from the domestic cat as a gamete source for in vitro embryo production as an animal model for a feline. The testes were collected from domestic cats during the sterilization procedures in a veterinary clinic and preserved for 72 hours at 4 degree celcius. The results showed that no difference was observed for testicular weight, epididymis weight, sperm count/individual, and sperm count/testis between right and left testis. There was no correlation between testis weight and epididymal sperm counts in this present study. Furthermore, the sperm motility, sperm viability, membrane sperm integrity, and sperm with normal morphology of fresh domestic cat in this study were 77.77%, 81.31%, 87.53%, 68.66%, respectively. Interestingly, during preservation at 4 degree celcius, generally the sperm epididymal motility and viability decreased according to preservation time. The percentage of sperm motility and viability significantly reduced about 50% in 72 hours of preservation compared to 5 hours preservation (75.5% vs 35.83% and 80.12% vs 33.13%). It can be concluded that cat sperm still have adequate motility and viability after preserved for 72 hours at 4 degree celcius so can be used as a sperm source for in vitro embryo production Keywords: preservation, sperm, motility, viability Share Link | Plain Format | Corresponding Author (Rini Widyastuti)

48	Veterinary Anatomy, Physiology, Pharmacology	ABS-80
Hepatoprotector Activity of Bligu Fruit Seed Extract (Benincasa hispida (thunb) cogn.) And Ants Plants Infusion (Myrmecodia pendans) in Rats (Rattus norvegicus) Induced by Paracetamol Khaerani (a*), Hurria (b), Faridha Yenny Nonci (a), Nurshalati Tahar (a), Munifah Wahyuddin (a), Asrul Ismail (a), Fathana Arif (a), Nur Amirah Reski (a) (a) Departement of Pharmacy, Medical and Health Sains Faculty, Universitas Islam Negeri Alauddin Makassar, Jalan Yasin Limpo 36 Gowa, South Sulawesi 90221, Indonesia *khaerani.mukhtar[at]uin-alauddin.ac.id (b) Department of Pharmacy, Faculty of Health Agriculture and Marine Science, Universitas Muhammadiyah Palopo, Jalan Jenderal Sudirman Km. 3 Kota Palopo, South Sulawesi 91921, Indonesia Abstract Hepatoprotectors from plants are preferred as alternative therapy because they are easier to obtain and safer. This study aims to determine the hepatoprotector activity of Bligu seeds extract (Benincasa hispida (Thunb) Cogn.) and ant plant infusion (Myrmecodia pendans) in rats (Rattus novergicus) induced by paracetamol using SGPT enzyme levels parameters. Bligu seeds were extracted using methanol while the ant plant made infusion, then tested on 15 rats wich were randomly divided into 5 tratment groups, that are the negative control group, the positive control group, the 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BW extract. And For Infusion, 100 g/kg BW, 200 g/kg BW and 300 g/kg BW. All groups induced using paracetamol 2,5 g/kgBW and given therapy for 7 days. SGPT levels were measured on the first day before treatment. After treatment continued with the induction of paracetamol wich waas given along follow up therapy. The results proved that the methanol extract of the seeds of Bligu (Benincasa hispida (Thunb) Cogn.) and the infusion of ant plant (Myrmecodia pendans) had a significance value > 0,05, which means that they have hepatoprotector activity where the extract dose is 400 mg/Kg BW for the Beligu seed extract and the dose 100 g/KgBW ant plant infusion were not signicantly different from the increase in SGPT levels on the effect of Curcuma suspension. Keywords: Bligu Seed- Ant Plant- Hepatoprotector Share Link | Plain Format | Corresponding Author (Khaerani Khaerani)

49	Veterinary Anatomy, Physiology, Pharmacology	ABS-87
POTENTIAL OVARY OF CATS BASED ON MORPHOLOGICAL NUMBER AND QUALITY OF OOCYTES IN LUTEAL AND NON-LUTEAL PERIODS Arief Boediono (a)*, Retno Saputro (a), Kusdiantoro Mohamad (a), Diah Nugrahani Pristihadi (a), Karisma Mardatillah (a), Wahyudin (a), Rini Widyastuti (a,b) (a)Departement of Anatomy, Physiology, and Pharmacology Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia *Corresponding author: ab[at]apps.ipb.ac.id Abstract Ovarian waste from sterilization in female cats through ovariohysterectomy surgery can be used as a source of oocytes in an effort to produce embryos in vitro. The purpose of this study was to evaluate the potential of female ovaries after sterilization based on the number of functional oocytes successfully collected in different follicular periods, namely the luteal period and the non-luteal period. Ovary of the cat was obtained from the sterilization action through ovariohysterectomy surgery. Ovaries were distinguished based on the presence of the corpus luteum (CL) of each pair of cat ovaries, namely the ovarian pair with CL (luteal period), and ovarian pairs without CL (non luteal period). Oocytes were collected by slicing the ovarian cortex. Oocytes were grouped into functional oocytes, fragmentation, spontaneous cleavage, and degeneration. The precentage of cat ovary that has CL and without CL were 59.1 and 40.9%, respectively. There was no difference in the number of CL between left and right ovary in the luteal period group. The number of functional oocytes that can be collected in the non-luteal period (27 oocytes per head) was greater (P<0.05) than the number that can be collected in the luteal period (13 oocytes per head). The results of this study are expected to be used as additional information for the utilization of wild ovaries of the Felidae family after death through embryo production technology in vitro. Keywords: cat, oocytes, ovariohysterektomy, ovary, luteal period Share Link | Plain Format | Corresponding Author (Rini Widyastuti)

50	Veterinary Anatomy, Physiology, Pharmacology	ABS-88
Antibiotic Resistance Test In Pseudomonas aeruginosa On Swiftlet Nest (Aerodramus fuciphagus) In Bone Regency Nurhashunatil Mar ah, Baso Yusuf , Rizalinda Sjahril Hasanuddin University Abstract Edible Bird Nest is one of Indonesia^s export commodities that has high economic value, which is known have beneficial to health made from lipids, ash, carbohydrates, and protein. In addition to these macronutrients, there are also bacterial contamination, which is one of them is Pseudomonas aeruginosa which is one of the bacteria with many mechanism of resistance and can cause diseases of the gastrointestinal tract. Bacteria in food products is a public health concern because it opposes the transfer of antibiotic-resistant foodborne pathogens to human populations. Gastrointestinal tract is an important entry point for the spread of these bacteria and the presence of antibiotic-resistant bacteria in food products is a concern for public health because it has the potential to move food-borne pathogenic bacteria. This study uses fifteen samples of edible bird nest that taken from five locations such as Pedesaan, Pegunungan, Persawahan, Daerah Dekat Laut, and Kota. Samples were cultured using BHIB and Mac Conkey Agar media, then identification and sensitivity testing were performed using vitek 2 compact. The results showed that Pseudomonas aeruginosa bacteria identified in the daerah pedesaan were stated to be sensitive to the antibiotics Piperacillin / Tazobactam, Ceftazidime, Cefepime, Aztreonam, Meropenenem, Amikacin, Gentamicin, Ciprofloxacin. The conclusion of this study is that edible bird nest found in Edible swiflet Nest Bird Houses in daerah pedesaan in Bone Regency are contaminated by Pseudomonas aeruginosa bacteria that are resistant to antibiotics Cefazolin and Tigecyline which is still classified as intrinsic resistance. Keywords: Edible Bird Nest, Pseudomonas aeruginosa, Susceptible,Vitek 2 Compact Share Link | Plain Format | Corresponding Author (Nurhashunatil Mar ah)

51	Veterinary Animal Disease and Veterinary Public Health	ABS-3
Occurrences of Escherichia coli in broiler meat from traditional market in Gowa regency, Indonesia Irma, Nuraeni, Sartika Juwita Politeknik Pembangunan Pertanian Gowa Abstract Contamination of microorganisms in broiler meat will cause the meat is easily damaged and rotten. One of the microorganisms that can cause damage and decay on broiler meat is Escherichia coli. The amount of risk caused by contamination of Escherichia coli bacteria is a major concern of consumers. This aim of study was to detect the presence or absence of contamination of Escherichia coli in broiler meat from traditional market in Gowa regency of South Sulawesi Province. TPC testing method refers to Indonesian National Standard (SNI) 012897-2008 and Escherichia coli contamination test refers to Table MPN series three tubes. The data is analyzed descriptively. The results showed that TPC (Total Plate Count) test detected 10 samples (41,67%) from 24 samples of broiler meat exceed maximum limit of microbial contamination 1x106 colony/g, while Escherichia coli contamination test detects 23 samples (95,83%) from 24 samples of broiler meat exceeded maximum limit of microbial contamination 1x101 colony/g. Keywords: Escherichia coli, broiler meat, TPC, MPN, traditional market Share Link | Plain Format | Corresponding Author (Sartika Juwita)

52	Veterinary Animal Disease and Veterinary Public Health	ABS-5
Effect of Chitosan Concentration on Spoilage Time of Broiler Chicken Meat Muhammad Dirga Gifardi (a*) Lucia Muslimin (a) Effendi Abustam (b) a) Veterinary Study Program, Faculty of Medicine, Hasanuddin University, Jalan Perintis Kemerdekaan km. 10, Makassar 90245, Indonesia *mdirga701[at]gmail.com b) Faculty of Animal Husbandry, Hasanuddin University, Jalan Perintis Kemerdekaan km. 10, Makassar 90245, Indonesia Abstract Broiler chicken meat is one of the sources of animal protein with a high level of consumption in Indonesia. Very high demand must be overcome with adequate production and distribution. However, it is not uncommon to find several problems in the process of producing broiler meat, one of which is the fast decomposing meat of broiler chicken. The solution to overcome these problems is the preservation process. One of the natural preservatives that can be used is chitosan. The purpose of this study was to determine the optimum concentration of chitosan to inhibit the spoilage of broiler chicken meat. The study was conducted on May to August 2018 at the Laboratory of Microbiology, Veterinary Medicine Study Program, Faculty of Medicine and Laboratory of Animal Product Technology, Faculty of Animal Husbandry, Hasanuddin University. This study was conducted with 6 treatments, namely control and soaking treatment in 1% acetic acid as a comparison, and soaking the meat in chitosan solution with concentrations of 0.5%, 1%, 1.5%, and 2%, each treatment consisting of 4 replications. Data were analyzed using descriptive methods and Analysis of Varience (ANOVA). The variables observed were time of decay, water binding capacity, and organoleptic. The results of the spoilage time showed that the concentration of chitosan 1%, 1.5%, and 2% could inhibit the spoilage time until the second day. The results of testing the water holding capacity showed that chitosan concentrations of 1.5% and 2% showed non-significant changes (p> 0.05) until the second day. The results of organoleptic testing showed that the concentration of chitosan was 2% most effective for maintaining the smell and taste of broiler chicken meat until the second day. Keywords: Broiler Chicken Meat, Chitosan, Spoilage Time Share Link | Plain Format | Corresponding Author (Muhammad Dirga Gifardi)

53	Veterinary Animal Disease and Veterinary Public Health	ABS-11
The effect of garlic extract (Allium sativum L) on the blood image of mice infected with Trypanosoma evansi Drh. Zainal Abidin Kholilullah, M.Kes- Drh. Adryani Ris, M.Si Program studi Kedokteran Hewan Fakultas Kedokteran Universitas Hasanuddin Abstract ABSTRACT One of the diseases that commonly attack livestock, especially horses and cattle is Surra disease. This disease is caused by infection with the parasite Trypanosoma evansi (T. evansi). In the animal^s body, T. evansi will enter the circulatory system and carry out breeding activities that result in damage to blood cells, especially red blood cells. Garlic (Allium sativum L) has been widely researched and the results are known to contain active compounds including flavonoids and Allicin (diallyl thiosulfinate). Allicin is one of the most biologically active components contained in garlic. This component, together with other sulfur components contained in garlic, has a role in giving garlic a distinctive odor. Allicin not only has antibacterial effects, but also anti- parasitic and antiviral effects (Londhe, 2011). Based on the Allicin content, we will test its activity on the blood image of mice as experimental animals that have been infected with Trypanosoma evansi parasite and then will evaluate its effect on the image of the mice^s blood. It is hoped that this research can be a source of information for researchers and the public about the benefits of garlic in treating trypanosomiasis cases. Keywords: Garlic Extract, Trypanosoma evansi, Blood Image, Mice Share Link | Plain Format | Corresponding Author (Zainal Abidin Kholilullah)

54	Veterinary Animal Disease and Veterinary Public Health	ABS-21
The causative factor detection and identification on Boophilus sp. tick infestation in bali cattle Yuliani Suparmin (a*), Dwi Kesuma Sari (a), Faizal Zakariya (b) a) Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan KM 10, Makassar 90245, Indonesia. * yuliani.suparmin[at]yahoo.co.id b) Veterinerinaria Farma Center (PUSVETMA), Jl. Ahmad Yani, Surabaya 60231, Indonesia. Abstract In tropical climates such as Indonesia, the presence of ectoparasites occurs almost annually, which becomes one of the major problems due to ectoparasite infestations on ruminant farms. One problem occurred is the Boophilus sp. The study unit was the Bali cattle breeders spreaded in Mallusetasi District, Barru Regency. This study used a cross-sectional study to identify and determine the relationship between the causative factors of maintenance management and stock farmer knowledge level on the application of maintenance management in Bali cattle farms. The tabulated data of the maintenance management in Bali cattle breeding and breeder knowledge level causative factors against the tick ectoparasite infestation were descriptively analyzed and tested with a chi-square (&#967-2) method to measure the relationship of these factors and Boophilus sp. tick infestation at 95% of confidence level. The magnitude of relationship strength was calculated by the odd ratio (OR) test at a 95% of confidence level. Boophilus sp. tick ectoparasite infestation occurred in Bali cattle farms in Mallusetasi District, Barru Regency with an incidence rate of 68.8%. The causative factors that influence the onset of tick infestations included the farmer educational level, breeder experience, number of maintenance, maintenance pattern, poor cage condition, and breeder^ knowledge. The infestation of Boophilus sp. tick had a very significant correlation on the onset of blood parasitic diseases. Keywords: Bali cattle- Boophilus sp- Ectoparasite- Infestation- Tick Share Link | Plain Format | Corresponding Author (Yuliani Suparmin)

55	Veterinary Animal Disease and Veterinary Public Health	ABS-23
Identification of Toxocara canis on Puppy in Makassar Pet Clinic Dian Fatmawati, Zulfikri Mustakdir, Dwi Kesuma Sari. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Makassar 90245, Sulawesi Selatan, Indonesia Abstract Toxocariasis in dogs is caused by the infection of Toxocara canis. The disease is not recognized and often underestimated by the owners. Toxocara canis is a pathogenic gastrointestinal worm and the larva could rush internal organs, cause diarrhea and kill the animals if the disease is not seriously handled . Toxocara canis is not only danger to the animals but it could also infect the human being. The purpose of this study was to identification Toxocara canis on puppy in Makassar Pet Clinic. Research done at July - August. Feces samples were collected from 31 puppies in Makassar Pet Clinic. Feces were examined by native method and floating method to detected eggs of Toxocara canis on the basis of there morphology. The result showed 5 samples were identified positive of Toxocara canis and 26 samples were negative of Toxocara canis. Keywords: Toxocariasis, Toxocara canis, dog, puppy Share Link | Plain Format | Corresponding Author (Zulfikri Mustakdir)

56	Veterinary Animal Disease and Veterinary Public Health	ABS-65
Identification of Endoparasites in Timor Deer (Cervus timorensis) Faeces at the Celebes Animal Citra Conservation Zoo, South Sulawesi C Tandirerung1, W S Monica2 (*), A Ris 3, Z Mustakdir 4 and Z Basrul5 1Veterinary Medicine Study Program, Hasanuddin University, Makassar, South Sulawesi, Indonesia 2Division of Veterinary Internal Medicine, Veterinary Medicine Study Program Hasanuddin University, Makassar, South Sulawesi, Indonesia *santawaode[at]med.unhas.ac.id 3Division of Veterinary Parasitology, Veterinary Medicine Study Program, Hasanuddin University, Makassar, South Sulawesi, Indonesia 4Division of Veterinary Parasitology, Veterinary Medicine Study Program, Hasanuddin University, Makassar, South Sulawesi, Indonesia 5Division of Animal Welfare, Hasanuddin University, Makassar, South Sulawesi, Indonesia Abstract Timor deer (Cervus timorensis) is one of Indonesia ruminant species whose natural habitat threatens with extinction, so ex-situ conservation efforts are needed. Conservation measures can experience the threat of failure due to disease, one of which is the parasitic disease factor. Lack of data related to disease information in Timor deer so that one of this study^s objectives was to identify endoparasites in Timor deer feces at the Celebes Animal Citra Conservation Zoo, South Sulawesi. The sample used in this study was ten Timor deer for feces collection. The Timor deer^s criteria that were sampled by a thin body decreased appetite and had dull hair. Samples were examined in the Hasanuddin University Veterinary Study Program^ of The integrated laboratory using three methods: the native method, the floating method, and the sediment method. Based on the results of observations under a microscope with a magnification of 40x, it found two positive samples of endoparasites with the characteristics of oval-shaped eggs, egg lengths ranging from 45.41 - 69.87 micron, having thick walls, and yellowish-brown eggs. The eggs found were Ascaris sp. The low number of positive samples in this study is partly due to the routine deworming program in animal conservation areas. Keywords: Parasite, Timor deer, Ascaris sp Share Link | Plain Format | Corresponding Author (Cristopel Tandirerung)

57	Veterinary Animal Disease and Veterinary Public Health	ABS-70
Antibody Response in Cattle After Local Isolate SE Vaccine Administration Susan M. Noor1, SS Prihandani, MI Desem, HHS. Purba, Andriani Balai Besar Penelitian Veteriner Abstract Septicemic Epizootica (SE) in livestock caused by bacterial infection of Pasteurella multocida. The serotype of P. multocida that causes SE in cattle and buffalo in Indonesia is the Asian serotype B: 2. The objective of this study is to develop of determining antibody responses of cattle after vaccinating with local isolates Local isolate SE Vaccine. Ten female cattle aged around one year used in this experiment. Cattle divided into 3 groups, Group A (4) vaccinated with local isolates SE vaccines, group B (4) vaccinated with commercial SE vaccines, and group C (2) unvaccinated. The concentration of SE vaccine is 2mg, and the vaccine was administrated 3ml sub-cutaneously. Antibody responses measured before and after vaccination using indirect-ELISA. The result of antibody response of cattle vaccinated with local isolate SE vaccine in seppic montanide formulation showed higher results compared to SE commercial vaccine and unvaccinated group. This local isolate SE vaccine has good potential as a candidate for SE vaccine in cattle. Keywords: Antibody, Cattle, Local isolate, SE, Vaccine Share Link | Plain Format | Corresponding Author (Susan Maphilindawati Noor)

58	Veterinary Animal Disease and Veterinary Public Health	ABS-71
The Prevalence of Ancylostoma spp in Young Dogs Under The Age of 12 Months in Hospital and Animal Clinic Surabaya Muhammad Muflih, Muchammad Yunus, Bambang Sektiari Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, Indonesia Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Airlangga University Department of Veterinary Clinic, Faculty of Veterinary Medicine, Airlangga University Abstract This study aimed to determine and compare the prevalence of Ancylostoma sp in young dogs under &#8804- 12 months, 0-4, 4-8, and 8-12 months from clinic and animal hospital in Surabaya. This study used 60 dogs faecal samples taken from two animals hospitals and two animals clinics in Surabaya. The fecal samples were examined by means methods i.e. native, sedimentation and floatation from the identify type of worm eggs we the found Ancylostoma sp and then calculate the prevalence rates from then analyzed by Chi Square. These results indicated that from 60 samples were examined 14 samples positive with the prevalence rate 23.33% in the group age 0-4 was 16.67% were positive, found as many as 4-8 month 23.53% were positive and at the age of 8-12 was 25.81%. Chi Square analysis showed there is no significant difference between the prevalence within group age 0,817 (p>0,05). Keywords: prevalence, Ancylostoma sp, young dog, animal clinic, animal hospital, Surabaya Share Link | Plain Format | Corresponding Author (Muhammad Muflih Nur)

59	Veterinary Animal Disease and Veterinary Public Health	ABS-77
The Influence of Newcastle disease (ND) Active La Sota Strain and B1 Strain Vaccinations toward Antibody Titer of Broiler) Muh. Danawir Alwi(a)*, A. Magfira Satya Apada (b), Fedri Rell (c) a. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, Indonesia b. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, Indonesia c. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, Indonesia Abstract Newcastle disease (ND) is a disease caused by the genus virus of Avian Paramyxovirus and it affects various types of poultry. Vaccination as a prevention attempt toward ND virus requires active and inactive vaccines. The purpose of this research is to determine the influence of ND active La Sota strain and B1 strain vaccinations toward antibody titer of the broiler. This research applies 54 samples of day old chicken (DOC) divided into three treatment categories. Vaccine administration is given for three-days-old samples by eye drops and blood samples from 7, 14, and 21-days-old samples obtained through vena brachialis. The result of the Hemagglutination Inhibition test analyzed using the Analysis of Variance (ANOVA) one-way method and continued by Least Significant Different (LSD) test if the calculated probability is (p< 0,05) while the data of immune presentation descriptively asserted in quantitative. The outcome of this research shows the vaccine administration for three-days-old sample grants protection of immune until 21-days-old by vaccine administration of ND active La Sota strain and 14-days-old with vaccine administration of ND active B1 strain. The vaccine administration of ND active La Sota has no significant effect on the vaccine administration of ND active B1 to antibody titer in broiler chickens. Keywords: Broiler, Newcastle disease (ND), La Sota strain vaccine, B1 strain vaccine, Hemaglutination (HA) test, Hemaglutination inhibition (HI) test Share Link | Plain Format | Corresponding Author (Muh. Danawir Alwi)

60	Veterinary Animal Disease and Veterinary Public Health	ABS-86
Aflatoxins analysis on uncleaned edible bird nest in South Sulawesi Baso Yusuf*1, Gagak Donny Satria2, Tri Wahyu Pangestiningsih2, Aris Haryanto2, Hery Wijayanto2, Agustina Dwi Wijayanti2, Osamu Kawamura3 1Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, 90245, South Sulawesi, Indonesia 2Faculty of Veterinary Medicine, Gadjah Mada University, Indonesia 3Faculty of Agriculture, Kagawa University, Japan Abstract Aflatoxin contamination was frequently found in animal feed or food due to the abiquity of the producing fungi. A study was conducted to analyze aflatoxins occurrence in uncleaned edible bird nest (EBN) obtained from edible nest swiftlet (Aerodramus fuciphagus) farmers in South Sulawesi, Indonesia. Three switlet houses were used to obtain EBN samples. The aflatoxins analysis was performed using in-house immunoaffinity column and high performance liquid chromatography-fluorescence detector (HPLC-FD). Of the 30 uncleaned EBN samples, none of aflatoxins were detected. This study indicates that all EBN samples were below the limit of detection and thus it could be classified as a low risk for human. However, this study should be compared to others studies related aflatoxins analysis in others circumstances to ensure the risk level of aflatoxins in edible bird nest. Keywords: edible bird nest, aflatoxin, in-house immunoaffinity column, high performance liquid chromatography-fluorescence detector Share Link | Plain Format | Corresponding Author (Baso Yusuf)

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