Preliminary Phytochemical Analysis and Antibacterial Activity of Aqueous Extract of Cassia siamea Leaves Againts Aeromonas hydrophila
Budianto Panjaitan1, Amalia Sutriana1*, Femi J. Sari1, Nuzul Asmilia1, Erina1

1Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, Indonesia

Abstract

This study aims to investigate the phytochemical analysis and in vitro antibacterial activity of aqueous extract of Cassia siamea against fish pathogenic Aeromonas hydrophila. Aqueous extract of Cassia siamea leaves were prepared and preliminary screened for phytochemical analysis. The different concentrations of the extract were then tested for their antibacterial activity against Aeromonas hydrophila using agar well diffusion method which was performed on Mueller Hinton Agar (MHA) plates. The phytochemical analysis showed that the aqueous extract of Cassia siamea contained alkaloid, flavanoids, phenols, steroids, tannin. The antimicrobial activity analysis showed that aqueous extracts of Cassia siamea at concentration of 40 %, 50, 60%, and 70% were able to inhibit the growth of A. hydrophila bacteria with zones of inhibition ranging from 10.40\pm0.36 mm to 13.47\pm0.5 mm as the lowest and highest zones of inhibition. The result of this study have supported the potential use of Cassia siamea as the natural antibacterial and recommend the possibility use of Cassia siamea in the treatment of fish disease caused by A. hydrophila.

Keywords: antibacterial activity, Cassia siamea, phytochemical screening

Topic: Biology and Biomedical Science