Histology of Sulawesi Medaka Fish Skin (Oryzias celebensis) in the Burn Healing Process
Andi Azifah Cahyani, Dwi Kesuma Sari, Irma Andriani

Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, South Sulawesi, Indonesia

Abstract

Medaka fish is a group of fish from the Actinopterygii class. One of the endemic medaka fish of South Sulawesi is the Sulawesi medaka fish Oryzias celebensis. Medaka fish are widely known as model fish in biology. This study aims to determine the histological description of the skin of the Sulawesi medaka in the healing process of burns. This study used the Sulawesi medaka fish which is a fish endemic to Sulawesi. The fish samples studied were divided into 3groups, group 1 as the control group(K1), group 2 namely the burn treatment group (K2) and then group3 (K3) namely a group of fish that were treated with burns and then kept in a container whose water has been dripped with methyleneblue. The fish in groups2 and 3 would then observe the wound healing process for 7days, namely on the 1st,3rd,5th, and 7th day. The fish were then immersed in formalin for 2days and then made preparations with the embedding blocking method with paraffin and HematoxylinEosin (HE) staining and analyzed descriptively qualitatively. Macroscopic observations of fish in the treatment group after giving burns (K2) on day 1 showed signs of widespread redness and the wound was still visible. On the 3rd,5th and 7th day there were no signs of redness and the wounds began to shrink on day 7.The results of observations in the treatment group with burns treatment coupled with the administration of methyleneblue (K3) on day 1 showed signs of redness and the wound is still visible. Day 3 the wound is not red and the wound is medium in size. On the5th day the wound started to get smaller and showed no signs of redness and swelling while on the7th day the redness was gone and the wound had begun to close. Observation of the histological features of the skin of the Sulawesi Medaka fish showed differences on day 1 to day 7. Inflammatory cells, fibroblast cells were found from day 1 to day7 with increasing numbers. Collagen fibers were found more on the 7th day while on the 7th day only granulation tissue.

Keywords: skin, medaka, Oryzias celebensis, histology, burn wound

Topic: Animal Laboratories