Histopathology of small gut (duodenum) of White Rat (Rattus Norvegicus) post ovariohysterectomy and orchiectomy
W S Monica (*), D K Sari, D Fatmawati

Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, South Sulawesi, Indonesia
*santawaode[at]med.unhas.ac.id

Abstract

This study aimed to determine the histopathological of the small intestine (duodenum) of white rats (Rattus norvegicus) after ovariohysterectomy and orchiectomy. In this study, 24 rats were used, consisting of 12 males and 12 females with a body weight ranging from 200-250 grams. The rat samples used were divided into four groups: the P0v group consists of the animals without ovariohysterectomy, the P1v group consists of animals with ovariohysterectomy, the P2r group consists of the animals without orchiectomy, and the P3r group consists of the animals with orchiectomy. Animals were adapted for one week, and then the animals were operated to remove the ovaries and testes. All groups were given the same feed or standard rat feed and observed for six weeks. Animals were euthanized for the small intestine (duodenum) sampling and then histology preparation was conducted using hematoxylin-eosin staining.

Keywords: histopathology, duodenum, Rattus norvegicus, ovariohysterectomy, orchiectomy

Topic: Animal Laboratories