Mangosteen Skin Extract (Garcinia mangostana L.) as anti-bacterial in Escherichia coli.
Abdul Wahid Jamaluddin, A. Magfira Satya Apada, Pusphita Sari

Corresponding author: abdulwahidjamaluddin[at]unhas.ac.id

Abstract

Mangosteen fruit skin with high antioxidant content has various potential as active medicinal substances, including antibacterial. Escherichia coli, which is commonly found in food and beverages and even animal feed, is ,of course a serious threat because in addition to the large issue of antibiotic resistance when using synthetic drugs, m E. coli can also cause foodborne diseases in the form of intestinal tissue necrosis, Hemorraghic colitis and Hemolytic uremic syndrome. For that we will test this potential mangosteen peel extract on E. coli bacteria and determine how its activity is to inhibit the growth of E. Coli. We use the following mangosteen rind extract concentrations for testing, namely 5%, 10%, 20% and 40% which was diluted using 10% DMSO then put into a blank disk, then the positive control used the antibiotic disk Amoxicillin plus clavulanate acid with a content of 30 micrograms and negative control using 10% DMSO. Testing was carried out in triple and tested on Escherichia coli bacteria using Muller Hinton Agar (MHA) media. The results of this study showed that the inhibition of E. coli starting from the extract of the skin for mangosteen 5% was 8.9 mm and the greatest inhibition was at a concentration of 40% of 16.8 mm. This mangosteen rind extract is effective in inhibiting the growth of the Escherichia coli bacteria.

Keywords: Mangosteen Skin Extract (Garcinia mangostana L.), antibacterial activity, Escherichia coli, in vitro

Topic: Biology and Biomedical Science