Histology of Sulawesi Medaka Fish Skin (Oryzias celebensis) in the Process of Healing Incision Wounds
Jessica Tania Loto, Dr. drh. Dwi Kesuma Sari, AP.Vet, Dr. Irma Andriani, S.Pi, M.Si

Hasanuddin University

Abstract

This study aims to determine the histology description of Sulawesi medaka (Oryzias celebensis) skin in the process of healing incision wounds. This research uses medaka fish which is a very famous experimental animal and has been widely used by researchers in the world to study in various fields of science, especially in biology and medicine. The samples studied were divided into 3 groups, namely group 1 as a negative control group (KP 1) without treatment as many as 6 Sulawesi medaka fish, group 2 namely the incision treatment group (KP 2) as much as 6 tails, group 3 as an incision treatment group and giving 6 Methylene blue (KP 3). After that the fish are kept in a 1 liter container and then maintained for 7 days. On the 7th day, all fishes were put into formalin to make preparations using Embedding blocking method with paraffin and Hematoxylin Eosin (HE) staining and analyzed descriptively qualitatively. Macroscopic observations showed that on day 1st the incision wound in the fish appeared reddish at the bottom of the wound. On the 7th day the incision wound was closed and the redness was not visible and there was no swelling for a period of 7 days. Microscopic observations showed that on day 7th an incision wound in fish showed signs of healing with improved skin epithelial forms.

Keywords: fish, histology, medaka, Oryzias celebensis, skin

Topic: Animal Laboratories