Identification of Aeromonas hydrophila Bacteria and its Effect to Histopathology of African Catfish (Clarias gariepinus) kidney
Ayu An Nisaa, A. Magfira Satya Apada, Dwi Kesuma Sari

Universitas Hasanuddin

Abstract

African Catfish is one of fresh water comodity that liked by many people so the african catfish demand is pretty high. The purpose of this study is to find out histopathology change of african catfish kidney that infected by Aeromonas hydrophila. Samples were used is 5 african catfish. Samples taken were catfish which showed clinical symptoms in the form of lesions on the surface of the skin, swollen abdomen and pale skin color. Aeromonas hydrophila identification was done by biochemical test using Vitek 2 Compact machine and the result is two out of five sample are positive infected by Aeromonas hydrophila. Preparations of kidney were fixed using 10% neutral buffered formalin (NBF), dehydration using graded alcohol, embedding using paraffin, cutting with a thickness of 5 &#956-m and stained with haematoxilin eosin and then observed. Analysis of the data used is descriptive qualitative. Based on observations obtained by damage or histopathology that occurs in the kidneys namely fat degeneration, connective tissue formation, necrosis, hemorrhage, infiltration of inflammatory cell and an increase in the number of melanomacrophages. Changes in histological structure that occur is a reaction from the body of catfish infected with Aeromonas hydrophila bacteria.

Keywords: Aeromonas hydrophila, African catfish, histopathology, kidney.

Topic: Marine Biology and Marine Disease