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61	Veterinary Clinic, Reproduction, Pathology	ABS-10
Wheelchair Prototype for Disabled Cat Henny Endah Anggraeni(a), Amata Fami(b) (a)Veterinary Paramedic Study Program, (b)Informatics management study program College Of Vocational Studies, IPB University Kampus IPB Cilibende Jl Kumbang No. 14 Bogor 16151 IPB University Abstract This study aims to produce a prototype (wheelchair) that can be used as a reference in making mobility aids for cats with disabilities. In the initial stage, the experimental animals were 5 local cats (Felis catus) 3-4 years old and weighing 3-4 kg for morphometric data collection. The prototype wheelchair produced is made of pvc pipe, with a size based on the morphometry of a local Indonesian cat. The wheelchair has a chest and abdominal support, equipped with two wheels on the back to support the hind legs, so the cat can move easily with the front legs. Prototype wheelchairs are made of safe and inexpensive materials, easy to use and help the mobility of cats with amputated legs or to support healing tissues. Keywords: Cat, prototype, wheelchair Share Link | Plain Format | Corresponding Author (Henny Endah Anggraeni)

62	Veterinary Clinic, Reproduction, Pathology	ABS-13
Morphology and Morphometry of Reproductive Organs in The Repeat Breeder Aceh Cows Sri Wahyuni*, Tongku Nizwan Siregar, Aqmarina Mazaya, Cut Nila Thasmi, Hafizuddin Hafizuddin, Mulyadi Adam, and Arman Sayuti Faculty of Veterinary Medicine, Universitas Syiah Kuala Banda Aceh *sriwahyuni[at]unsyiah.ac.id Abstract The aim of this study was to determine the morphology and morphometry of the reproductive organs of Aceh cows that experience the repeat breeding (RB). This study used samples of reproductive organs from RB and non-RB (fertile) Aceh cows, each of three cows with body weights of 150-200 kg and aged 3-6 years. Samples were obtained from a local slaughterhouse, including the ovarium, oviduct, cornua, corpus, and cervix uteri, vagina, and vulva. The criteria of RB and fertile Aceh cows were obtained from inseminators reports. Morphological and morphometric observations were performed on ovaries, oviduct, cornua, corpus, and cervix uteri, vagina, and vulva. The results showed that morphologically there were observed differences in ovaries of RB cows namely the presence of ovarian cysts and swelling in cornua uteri that accompanied by pyometra and hydrometra, and also deformation of cervix uteri. According to morphometric observation, the diameter of cornua uteri dexter, cervix uteri, and vulva of RB cows was bigger than non-RB cows (P<0.05). It can be concluded that morphology and morphometry of reproductive organs of RB and non-RB Aceh cows are different except for the oviduct. Keywords: Repeat breeding, Reproductive organ, Aceh Cow Share Link | Plain Format | Corresponding Author (Sri Wahyuni)

63	Veterinary Clinic, Reproduction, Pathology	ABS-14
The effect of time interval differences between the prostaglandin f2 alpha injection and semen collection on the improvement of spermatozoa and testosterone concentrations in Aceh Bull BUDIANTO PANJAITAN, VALERIE YOPPY EDWARD, DWI ATMAJA NISHCAYA, HUSNURRIZAL HUSNURRIZAL, TEUKU ARMANSYAH, MUSLIM AKMAL, TONGKU NIZWAN SIREGAR, HERRIALFIAN Faculty of Veterinary Medicine, Universitas Syiah Kuala, Aceh, Indonesia Abstract The injection of prostaglandin F2 alpha (PGF2\alpha) has been adopted as an alternative to improve sperm quality, and the difference in sample collection time after administration is assumed to have a positive effect on testosterone concentration. This research was aimed at determining the effect of PGF2\alpha injection and the optimal time for sample collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into 2 treatments. In treatment 1 (P1), the bull received 25.0 mg PGF2\alpha (Lutalyse, dinoprost, thromethamin) 1 hr prior to semen collection, while in treatment 2, the semen collection was carried out 2 hrs after PGF2\alpha administration. The treatment was performed five times on alternate weeks Semen collection was performed using an artificial vagina once a week for ten week Testosterone concentrations were analyzed using the enzyme linked immunosorbent assay (ELISA) method. The average spermatozoa volume (mL), concentration (106 cells/mL), and testosterone concentration (ng/mL) in P1 vs P2, were 4.82\pm1.93 vs 4.92\pm2.45, 889.0\pm250.50 vs 721.0\pm246.25 (P<0.05), and 8.35\pm2.34 vs 9.60\pm2.24 (P>0.05), respectively. It is concluded that the PGF2\alpha injection 1 hour prior to collection was able to increase the spermatozoa concentration compared to the 2 hours treatment, while the value obtained for testosterone was not affected. Keywords: Aceh bull, PGF2 alpha, testosterone Share Link | Plain Format | Corresponding Author (Budianto Panjaitan)

64	Veterinary Clinic, Reproduction, Pathology	ABS-17
Protein Profiles of Seminal Plasma and their Correlation with Semen Quality in Aceh Bull Hafizuddin (a*), Teuku Zahrial Helmi (a), Ginta Riady (a), Husnurrizal (a), Muslim Akmal (a), Zul Azmi (b), Yusmadi (c, d), Muhtar (d), Jauhari (d), Munzir (d) a) Faculty of Veterinary Medicine Universitas Syiah Kuala, Jl. Teungku Hasan Krueng Kalee No.4, Banda Aceh 23111, Indonesia *hafizuddin_umar[at]unsyiah.ac.id b) Indonesian Research Center for Veterinary Sciences, Jl. RE. Martadinata Haji Bin Ali No.30, Kota Bogor 16124, Indonesia c) Department of Animal Science, Faculty of Agriculture, Almuslim University, Jl. Jalan Kampus Timur, Matangglumpangdua, Bireuen 24261, Indonesia d) Indrapuri Livestock Breeding and Forage Centre, Jl. Banda Aceh - Medan KM. 24, Indrapuri, Aceh Besar 23373, Indonesia Abstract This study aims to characterize the protein profile of seminal plasma in Aceh bulls and to determine its correlation with semen quality. A total of four bulls (24-34 months) belonging to the Indrapuri Livestock Breeding and Forage Center were selected randomly. An aliquot (1 ml) from each ejaculation was used for the evaluation of semen quality (semen volume, mass motility, sperm motility, sperm viability and sperm morphology). The remaining volume of semen was centrifuged to obtain seminal plasma. Total seminal plasma protein concentration was calculated using Bradford method, then the seminal plasma protein was electrophoresed on SDS-PAGE and visualized with coomasie blue. The results of protein visualization found 16 protein bands with different molecular weights, ranging from 11 to 180 kilo Dalton. In general, the protein band of 15.24 kilo Dalton was more prominent in Aceh bulls. In addition, the seminal plasma protein concentration showed a positive correlation with sperm motility, sperm viability, and sperm morphology. In conclusion, the seminal plasma protein of Aceh bulls is positively correlated with several semen quality variables and may be a useful as additional parameter for determining semen quality or bulls fertility. Keywords: Protein profiles, Seminal plasma, Semen characteristics, Aceh cattle, Bull fertility Share Link | Plain Format | Corresponding Author (Hafizuddin Hafizuddin)

65	Veterinary Clinic, Reproduction, Pathology	ABS-18
Description of histochemical of gills and kidney betok fish (Anabas testudineus Bloch, 1792) at Tunggu Pampang Makassar reservoir Wastuti Aritonang1, Dwi Kesuma Sari1*, Irma Andriani2 1. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan. KM. 10, Makassar, 90245, Indonesia 2. Study Program of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Jl. Perintis Kemerdekaan KM.10, Makassar, 90245, Indonesia *E-mail: dwiksari[at]vet.unhas.ac.id Abstract Histochemistry is the study of tissue staining in histological staining. This study aims to analyze histological structures specifically with histochemical techniques of gill and kidney fish (Anabas testudineus) in reservoir Tunggu Pampang Makassar. The research method is experimental descriptive. This research was conducted in August - September 2018 at the Animal Clinical Pathology Laboratory of Hasanuddin University Education, using a sample of 6 Anabas testudineus fish which have a length of 12-13.5 cm with an average of 12.6 cm and a weight size of 28 , 9 - 29.6 g with an average of 29 g of fishermens catch in the waters of the Tunggu Pampang reservoir. Histological preparations were made using the paraffin method and histochemical staining, namely Hematoxylin-Eosin (HE), alcian blue (AB) periodic acid schiff (PAS). The results showed a difference in the results of staining on the gills and kidneys of betok fish. Hematoxylin - eosin (HE) staining shows the histological structure of gills and kidneys. The coloring of Alcian blue (AB) pH 2.5 in the gills and kidneys of betok fish shows the concentration of distribution of acidic carbohydrates marked in blue which shows a positive reaction. Meanwhile, staining of Periodic Acid Schiff (PAS) shows a positive reaction to the gills and kidneys which are characterized by the formation of mangenta color. Keywords: Alcian blue, kidney, hematoxylin - eosin, histochemistry, histology, gills, Periodic Acid Schiff. Share Link | Plain Format | Corresponding Author (Wastuti Aritonang)

66	Veterinary Clinic, Reproduction, Pathology	ABS-24
MERCURY CHLORIDE (HGCL2) AFFECTS THE ANATOMICAL PATHOLOGY AND HISTOPATHOLOGY OF MALE TILAPIA (OREOCHROMIS MOSSAMBICUS) GONAD Nazaruddin1, Dwinna Aliza1, Teuku Armansyah2, Roslizawaty3, Erdiansyah Rahmi4, Nuzul Asmilia3, Putika Sari Ayu5 1Pathology Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh 2Pharmacology Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh 3Clinical Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh 4Histology Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh 5Veterinary Medicine Study Program, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh Corresponding author: Dwinna Aliza, email: dwinna.aliza[at]unsyiah.ac.id Abstract In fishes, Hg can inhibit gametogenesis, induce testicular atrophy, and impair individual reproduction. Despite some of Hg induced damages are known, there is still less information about the metal accumulation on tissue and its relationship with tissue structure disorganization of the fish testis, especially for tropical species. This study aimed to determine the effect of HgCl2 exposure on anatomical pathology and histopathology of male tilapia fish (Oreochromis mossambicus) gonad. A total of 36 male tilapia fish were allotted into 4 treatment groups with 3 replications. Fish were exposed to 0.00, 0.25, 0.50, and 0.75 ppm of HgCl2 for 10, 20, and 30 days. Fish gonad appearance was observed followed by histopathological examination using hematoxylin and eosin staining. The anatomical pathology examination of fish gonad demonstrated several changes from atrophy, hypertrophy, to necrosis. The histopathological observation showed that the lowest averages of spermatozoa spreading in seminiferous tubule were shown in group K4, and the highest average of gonad cells necrosis number was observed in group K4 with 30 days exposure. Statistically, both histopathology parameters were significantly different compare to the control group. In conclusion, HgCl2 exposure changes the anatomical pathology and histopathology of male tilapia fish gonad. The higher the HgCl2 concentration and the longest time of exposure the more severe the damage of the fish gonad. Keywords: mercury, fish male gonad, histopathology, necrosis, athrophy Share Link | Plain Format | Corresponding Author (Dwinna Aliza)

67	Veterinary Clinic, Reproduction, Pathology	ABS-28
Epidemiological Analysis of Ovarium Hypofunction in Beef Cattle in Jepara Regency Aldi Salman (a*), Surya Agus Prihatno (b), Bambang Sumiarto (b) (a) Office of Livestock and Animal Health, Central Java Province Komplek Tarubudaya, Jalan Gatot Subroto, Ungaran, Central Java (b) Faculty of Veterinary Medicine, Universitas Gadjah Mada Jl. Fauna 2 Karangmalang, Sleman, Yogyakarta Abstract Ovarian hypofunction is the highest incidence of reproductive disorders in Central Java, breeders losses due to the long calving interval and increased maintenance costs. Feed quality is considered to be a cause of ovarian hypofunction, but there are also other factors that cause disease. The purpose of this study was to determine the prevalence and risk factors for ovarian hypofunction in beef cattle, as well as a model for predicting ovarian hypofunction in Jepara Regency. A total of 304 beef cow from 176 breeders were selected by formal random sampling in 14 villages in 7 sub-districts with multiple stages. Anamnesis was conducted to farmers and rectal examination of beef cow to determine the reproductive status, and questionnaires for animal husbandry and individual animals level. The results showed prevalence of ovarian hypofunction 8.88% and risk factors that have a relationship are frequency of supplementary feed (OR = 12.77), ownership of one animal (OR = 0.34), main feed (OR = 9.59), breed type (OR = 0.37), cows age (OR = 0.33), lactation status (OR = 0.07) and calf weaning time (OR = 0.03). Model of disease formed are Ln P/1-P = 0,484 + (2,247xPKUT) + (-4,406xSAPH). Keywords: ovarian hypofunction, beef cattle, prevalence, risk factor, model of disease Share Link | Plain Format | Corresponding Author (Aldi Salman)

68	Veterinary Clinic, Reproduction, Pathology	ABS-33
Docking and herbal treatment for equine melanoma in an Arabian Horse A. Amrozi1*, R.H. Soehartono1, E.R. Adistya2, I Mahardi3, H. Mukarromah3, M.I. Satria3, B. Achmadi1, K. Karthikeyan1 1*Departemen Klinik Reproduksi dan Patologi, Fakultas Kedokteran Hewan, IPB, Bogor 2Rumah Sakit Hewan Pendidikan, Fakultas Kedokteran Hewan, IPB, Bogor 3Dr Am Veterinary Services, Jl Ketimun C93, Sinarsari, Dramaga, Bogor Abstract Abstract. Equine melanoma is a condition that arises frequently in horses with a grey coat color than in horses of other colors. In general terms, melanoma is also known as a neoplasm. These tumors can be either formed as benign and malignant which can be classified by examining their histological and macroscopic features. In this case of necrotic equine melanoma was found in coccygeal bone an 20-year-old grey Arabian Mare. The mare was hand over in chronic condition of a bleeding tail, pus containing, abnormal growth of the tumor, and the initial therapy was directed with antibiotics, anti-inflammatory, balanced nutrition, and good care of wound management in the mare. Tail amputation or docking was performed to treat the horse and followed by herbal treatment by using turmeric powder. Curcumin is the main compound found in turmeric powder. Curcumin has been shown to have anti-inflammatory, anti-oxidant, anti-carcinogenic, anti-mutagenic, anticoagulant, and anti-infectious effects. The excision of melanoma tumors in horses could be a practicable treatment option that is locally curative with minimal complication. Factors affecting the decision for surgery include the rate of growth of the lesion, age of the horse, presence of necrosis/infection, functional compromise, and potential future complication. It is advisable to remove tumor growth in potentially problematic areas before they become large enough to cause a clinical predicament. Keywords: equine melanoma, docking, turmeric Share Link | Plain Format | Corresponding Author (Amrozi Amrozi)

69	Veterinary Clinic, Reproduction, Pathology	ABS-37
Determination of ovulation time in gayo mares based on image of preovulatory follicle growth Juli Melia 1), Nuzul Asmilia 1), Amrozi Amrozi 2), Iman Supriatna 2), Muhammad Agil 2) 1) Laboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Jl. T. Hasan Krueng Kalee No. 4 Kopelma Darussalam Banda Aceh 23111, Indonesia 2) Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University, Jl. Agatis Dramaga Bogor 16680, Indonesia Abstract This study aimed to obtain the ultrasound image of preovulatory follicle growth in gayo mare to predict the ovulation time. This study used three gayo mares and one gayo stallion for teaser aged 5-10 years and weighed 200-250 kg. Preovulatory follicle growth was observed using transrectal ultrasonography which was performed when the dominant follicle size was \geq 3.5 cm. Observations were made for 3 consecutive estrous periods on gayo mares at relatively the same time. The absence of previous follicular appearance and the presence of hypoechoic area in the same ovary indicate that ovulation has occurred. The result revealed that the diameter of preovulatory follicle of gayo mares was 4.02\pm0.32 to 5.05\pm0.04 cm. There was no significant follicle growth occurred in the last 48 hours before ovulation and the preovulatory follicle shape altered from spherical to pearshape. Many small follicles (2 cm in diameter) were observed in peripheral area of the ovary indicated the recruitment of follicles which form the first follicular wave. To conclude, the alteration in the size and pearshape of preovulatory follicle and the recruitment of small follicles can be used as a marker to determine the time of ovulation on gayo mares. Keywords: gayo mare, estrous, preovulatory follicle, ultrasound Share Link | Plain Format | Corresponding Author (Juli Melia)

70	Veterinary Clinic, Reproduction, Pathology	ABS-38
Subclinical hypertrophy cardiomyopathy in young adult mini pomeranian Arni Diana Fitri1,2,3, Fitria Senja2, Nindya Dwi Utami2, Rr Soesatyoratih1,3, Andriyanto1,4, M Fakhrul Ulum1,3, Deni Noviana1,3 1 Veterinary Teaching Hospital, Faculty of Veterinary Medicine, IPB University 2 Cardiology Center Services, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, IPB University 3 Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University 4 Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University Abstract Heart disease is a disease that is quite common in dogs, especially small dog breeds. Most cases of dog heart disease are only recognized by the owner after an advanced stage i.e. the animal showed symptoms of prolonged cough and comprehensive examination at the clinic. The case study aimed to present the subclinical heart disease found in a young adult dog. This heart case was experienced by a 4-year-old male mini Pomeranian dog. This Pet was referred for a heart check-up because the referring doctor found an abnormal heart sound. After a comprehensive examination such as physical examination, thorax radiography, electrocardiography, and echocardiography was performed, abnormalities were found then confirmed the patient has a heart problem. From the results of the physical examination, there was no evidence of coughing, and no changed activity levels. There were normal retina vessels and mucous membrane color, also there was no changed of respiratory rate and effort. The results of the radiography examination showed a heart enlargement compared to normal. Electrocardiography (ECG) examination results showed abnormalities in the R-R interval and QRS complex. The ECG formed sinus arrhythmia. The echocardiography results showed thickening of the left ventricle wall and sinus bradycardia. From the abnormalities found in this dog, it was concluded that these animals had mild hypertrophy cardiomyopathy (HCM) disease. In this case, the patient should have regular heart check-ups to monitor the progression of the disease. Keywords: Hypertrophy cardiomyopathy, sinus bradycardia, sinus arrhythmia, mini Pomeranian dog. Share Link | Plain Format | Corresponding Author (Arni Fitri)

71	Veterinary Clinic, Reproduction, Pathology	ABS-43
Inflammatory responses in the mammary gland after intrauterine infusion of lipopolysaccharide in dexamethasone-treated goats Fika Yuliza Purba(1,3*), Takahiro Nii(2), Yukinori Yoshimura(2), Naoki Isobe(2) (1)Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan (2)Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan (3)Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia Abstract Our previous study showed that intrauterine-infused lipopolysaccharide (LPS) can be translocated to the mammary gland to induce weak inflammation. This study aimed to determine if dexamethasone treatment facilitated the translocation of LPS from the uterus to the mammary gland to induce a heavy inflammatory response. Sixteen goats were divided into control and LPS groups (n = 8 each), subjected to daily dexamethasone administration before saline or LPS infusion. Milk and blood samples were collected before and after LPS infusion. Mammary gland tissues (n=2) were collected before and 24 h after LPS infusion for immunohistochemical analysis of LPS. The mean SCC in the LPS group was significantly higher, whereas the milk yield was significantly lower than that in the control group after LPS infusion. The mean BLC in the LPS group was significantly lower than in the control group after LPS infusion. Furthermore, milk concentrations of IL-1&#946-, S100A8, and lactoferrin were higher in the LPS group than in the control group after infusion. LPS was detected in the connective tissues and inner alveolar spaces of the mammary glands 24 h after LPS infusion. We concluded that dexamethasone administration facilitated the translocation of intrauterine-infused LPS to the mammary gland, where it induced an inflammatory response. Keywords: dexamethasone, lipopolysaccharide, mastitis, cytokine, antimicrobial peptide Share Link | Plain Format | Corresponding Author (Fika Yuliza Purba)

72	Veterinary Clinic, Reproduction, Pathology	ABS-45
Molecular Analysis of BMP 15 Gene in Sumba Ongole Cattle, Sumba Island, East Nusa Tenggara Cynthia Dewi Gaina1*, Maxs U. E.Sanam1, Marlene WM Nalley2, Imanuel Benu2, Agus Saputra1 1 Faculty of Veterinary Medicine, Nusa Cendana University, Indonesia 2 Faculty of Animal Science, Nusa Cendana University, Indonesia Abstract Sumba Ongole cattle are one of local beef cow in Indonesia that has been known for its benefit as source of protein and highly adaptive to extreme climate, like Sumba Island. Many genes are known to have an effect on reproductive performances traits, but the role of one gene, BMP 15 has not been explored yet. This gene is responsible for regulating ovarian function in cows. Due to the rare information about this gene characteristic, this study aimed to identify BMP 15 gene in Sumba Ongole (SO) cattle and its functions on reproduction system. The blood sample from 47 SO cattle which reared extensively in Sumba Island, were collected and were analyzed. The method used in this research was polymerase chain reaction (PCR), electrophoresis and DNA Sequencing. All DNA samples were amplified using BMP15 primer for 35 cycles and amplicons were visualized on 1.5% agarose gel. A total of 47 samples have been sequenced in PCR machine. The BMP 15 gene forward primer was 5^-AGTTTGTACTGAGCCGGTCT-3^ and reverse primer was 5^-CTGACACACGAAGCGGAGT-3^. The software Basic Alignment Local Search Tool (BLAST) was used to sequence and to analyses. The results revealed that the size of all the fragments was in accordance with that of BMP 15 and it was not found any genetic variations among the samples. Keywords: BMP 15, Gene, SO, Cattle, Sumba Share Link | Plain Format | Corresponding Author (Cynthia Dewi Gaina)

73	Veterinary Clinic, Reproduction, Pathology	ABS-54
Reproductive performance of buffaloes (Bubalus bubalis) in Baraka Enrekang South Sulawesi Indonesia Mukhammad Yusuf Kadir Pole1, Suhartila2, Zainal Abidin Kholilullah1, Lucia Muslimin1, Fika Yuliza Purba1,3* 1. Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia (ymukhammad[at]gmail.com) 2. Livestock and Fisheries Service of Enrekang Regency, Galonta, Enrekang, Enrekang Regency, South Sulawesi, 91712, Indonesia 3/ Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan (fikapurba[at]med.unhas.ac.id) Abstract Water buffalo is one of the most productive domestic animals with a high economic value and can easily adapt to its environment. This study aims to investigate the reproductive performance of water buffaloes in Baraka, Enrekang, South Sulawesi. Our investigation was conducted by data collection and interviews with the breeders. Seventy-seven females water buffaloes from four villages: Bone-Bone, Kendenan, Salukanan and Pepandungan were studied. The reproductive performance of the water buffaloes was assessed by several parameters, such as the age at first estrus, pregnancy length, age at first calving, calving interval, and calving to postpartum estrus interval. The age at the first estrus was 3.59 years old, the pregnancy length was 11.11 months, the age at first calving was 4.41 years old, the calving interval was 2.04 years, and the calving to postpartum estrus interval was 30.11 days. We concluded that the studied water buffaloes in Baraka, Enrekang were in a good reproductive condition. The results were supported by the field observations, which indicated the prime condition of the water buffaloes rearing system. Keywords: water buffalo, reproductive performance, Enrekang Share Link | Plain Format | Corresponding Author (MUKHAMMAD YUSUF KADIR)

74	Veterinary Clinic, Reproduction, Pathology	ABS-66
Ultrasonography and Pyelography of a Mixed-Breed Dog with Bilateral Hydronephrosis and Bilateral Hydroureter without any Clinical Sign Nurul Annisa Tuliman1), Ardi Diana Fitri 1), Fitria Senja Murtiningrum1) , Nindya Utami1), Sitaria Siallagan 1), Hamdika Yendri 1), Noor Ihsan Anzary Bahtiar 1), Rr Soesatyoratih1,2), Andriyanto1,3), Deni Noviana 1,2) 1)Diagnostic Imaging Centre, IPB University Veterinary Teaching Hospital, Faculty of Veterinary Medicine, IPB University 2)Department of Veterinary Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University 3)Department of Anatomy, Physiology and Pharmacology, Faculty of Veterinary Medicine, IPB University Abstract Hydronephrosis and hydrouereter is a condition that result from complete or partial obstruction of the urinary flow. This paper report the case of a five-year-old, mixed breed dog diagnosed with bilateral hydronephrosis and bilateral hydroureter by using ultrasonography and intravenous pyelography (IVP) without spesific clinical symptoms. The dog was referred to Veterinary Teaching Hospital Faculty of Veterinary Medicine of IPB University for routine general check up. The routine general check up for the patient are clinical exam, complete blood count (CBC) and serum biochemical panel, with ultrasonography. On the clinical examination, CBC, and serum biochemical panel were revealed no abnormal findings, but the USG revealed abnormality in the kidney and ureter. By the ultrasonographic examination revealed that bilateral enlarged kidney and hydronephrosis (right kidney 3.70 cm- left kidney 3.20 cm), moderate renal pelvic dilatation with kidney stone, and extremely dilated ureter (right ureter 0.63 cm- left ureter 0.37 cm). For confirmation, Intravenous Pyelography (IVP) technique was performed. The IVP contrast material (iopamidol) was reached the kidneys, ureters, and vesica urinaria 15 minutes after injection. IVP examination revealed that bilateral hydronephrosis, hydoureter (right is greater than left), ectopic ureter, and urolithiasis. The recommended treatment for hydroureter is supportive fluid therapy, diagnosis cause of obstruction and when possible, reestablishment of urinary flow, and general routine check up are required Keywords: Kidney, Hydronephrosis, Hydroureter, Ultrasonography, Intravenous Pyelography Share Link | Plain Format | Corresponding Author (Nurul Annisa Tuliman)

75	Veterinary Clinic, Reproduction, Pathology	ABS-67
Systemic and Local Expression of Endothelin-1 Lead to Early Renal Impairment In High Fat Induced Wistar Rats Haslindah Dahlan(1), Muhammad Husni Cangara(1), Rina Masadah(1), Irfan Idris(2), Upik A Miskad(1), Andi Alfian Zainuddin(3), Miswani Syuaib(1), Takao Fujimura(4) 1Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia 2Department of Physiology, Faculty of Medicine Hasanuddin University, Makassar Indonesia 3Department of Public Health, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia 4Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan Abstract The pathogenesis of chronic kidney disease in obesity is being suspected of having a strong relationship with the role of Endothelin-1 (ET-1). This study aims to assess levels of expression ET-1 in serum and renal tissue associated with changes in kidney structure. The rats were divided into two groups. The group that was given a high-fat diet and the control were assigned a standard diet. The serum of blood checked for ET-I using ELISA methods and saw the distribution of ET-1 expression by Immunohistochemistry (IHC) and Periodic Acid Schiff (PAS) staining from the paraffin-blocked kidneys. ET-1 levels in the serum of high fat-induced rat groups were higher than the control (p<0,05) as well as in glomeruli of the renal tissue (p<0,05). PAS staining enhancement of basal expansion is higher in High Fat induced rats than the control (p<0,05). These data confirm the role of ET-1 will provide changes in the levels of tissue and serum levels in obesity di early kidney injury. Keywords: Endothelin-1, obesity, early kidney injury, chronic renal failure Share Link | Plain Format | Corresponding Author (Haslindah Dahlan)

76	Veterinary Clinic, Reproduction, Pathology	ABS-68
The first successful combination of parenteral and inhalation anesthesia of GTCT (Granulosa Theca Cell Tumor) surgery in horses at Animal Teaching Hospital, IPB University Erly Rizka Adistya1*, Amrozi2, Deni Noviana1,2, Arni Diana Fitri1, Hamdika Yendi Putra1 1 Veterinary Teaching Hospital, Faculty of Veterinary Medicine, IPB University 2 Department of Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine, IPB University Abstract High morbidity and mortality during general anesthesia imply that anesthesia in horses must be carried out appropriately. The longer the animal is under anesthesia, the higher the morbidity and mortality. This paper reports on one successful case of using a combination of parenteral anesthesia and inhalation anesthesia in surgery performed at the Veterinary Teaching Hospital, IPB University. This combined anesthesia was used in GTCT surgery of the mare horse, 14 years old, thoroughbred came with a history of stallion-like aggression, anestrous, and nymphomania. Transrectal ultrasonography was the performed and found the right ovary was grossly enlarged. The diagnosis of GTCT was then confirmed. Blood panel test was performed before the surgery. A combination of parenteral anesthesia and inhalation anesthesia was carried out in this GTCT removal surgery on September 11, 2019. Parenteral general anesthesia was administered as induction and maintenance of anesthesia using inhalation anesthesia (Isoflurane) of 2 - 2.5% MAC which was given with oxygen to the patient via ETT (Endotracheal Tube). The use of combined parenteral anesthetics and inhalation anesthetics, in this case, was successfully seen from physical parameters during surgery until post-surgery was within the safe range (HR 32-44 BPM- RR 8-12 x /min- T 36oC - 37.8oC- and SPO2 94 % - 99 %) and the animal recovers 10 - 15 min after the inhalation anesthetic is turned off. This is the first successful combination of parenteral and inhalation anesthesia performed in GTCT surgery in Indonesia. Keywords: inhalation anesthesia, horse, GTCT surgery Share Link | Plain Format | Corresponding Author (Erly Rizka Adistya)

77	Veterinary Clinic, Reproduction, Pathology	ABS-72
The Aplication of Rapid Immunohistochemistry Test (RIT) Method on Dog^s Brain Tissues Suspected Rabies in South Sulawesi Wahyuni, Rizalinda Sjahril, Dwi Kesuma Sari Balai Besar Veteriner Maros, Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Department of Microbiology, Faculty of Medicine, Hasanuddin University Abstract The aim of the research was to find out the sensitivity and spesifity RIT method on dog^s brain tissues suspected rabies and distribution of rabies antigen on part of the brains. Thirty brains of dogs needed for this study with devided three part were cerebrum, cerebellum and hippocampus so total to be ninety smear of dogs brains. Statistic analysis caunted sensitivity and spesifity of RIT compared with Histopathology and FAT (Flourescent Antibody Test) for Gold Standard as antigen distribution on part of brains with standard error graphic and t test. The results were RIT method had sensitivity the same of FAT (100%) and better then histophatology (93%) and the higher of distribution rabies antigen were hippocampus, cerebrum and the last cerebellum. Keywords: zoonosis, disease, brain, Rabies Share Link | Plain Format | Corresponding Author (Wahyuni Wahyuni)

78	Veterinary Clinic, Reproduction, Pathology	ABS-73
Evaluation of semen quality of horses in Jeneponto South Sulawesi Indonesia Muhammad Abdi Awal 1 , Bungsuhari Burhan 2 , Muhammad Yusuf 3 , Lucia Muslimin 1 , Fika Yuliza Purba 1,4* (1) Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Makassar, 90245, Indonesia (2) Agriculture Service of Jeneponto Regency, Balang Toa, Binamu, Jeneponto Regency, South Sulawesi, 92315, Indonesia (3) Faculty of Animal Science, Hasanuddin University, Makassar, 90245, Indonesia (4) Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528, Japan Abstract Efforts to increase the population of high-quality horses in South Sulawesi is carried out through the application of artificial insemination (AI) technology. Currently, the AI in horses has been limited done using imported frozen semen. The production of local semen is encouraged to maintain the sustainability of AI practice. This study was carried out to elucidate the quality of fresh semen collected from racehorses and workhorses in Jeneponto. Twelve stallions were used in this study, consisted of 6 racehorses and workhorses, respectively. The quality of fresh semen was assessed by examining the semen characteristics and sperm quantity and quality. The results showed that the semen volume of racehorses and workhorses was 44.25 mL and 51.02 mL, respectively. Horses semen had white-milky color and watery consistency with pH 7. The microscopic observations revealed that the sperm motility of racehorses and workhorses was 51.02 and 49.9, the life sperm percentage was 53.5 and 57.43, the concentration of sperm was 151.66x106/mL and 190.83x106/mL, the sperm abnormalities percentage was 23.51 and 31.07, respectively. We conclude that the quality of racehorses and workhorses fresh semen were only different in sperm abnormalities percentage. Keywords: horse, racehorse, semen evaluation, sperm Share Link | Plain Format | Corresponding Author (Muhammad Abdi Awal)

79	Veterinary Clinic, Reproduction, Pathology	ABS-89
Effects of storage duration on Mitochondrial activity and DNA fragmentation of post-thawed spermatozoa from several Ongole Grade bull in Indonesia Kurniawan Dwi Prihantoko1, A. Kusumawati2, D.T. Widayati3, Mulyoto Pangestu4, 1Doctoral Student of Veterinary Science, Faculty of Veterinary Medicine, University Gadjah Mada, Yogyakarta, 55281, Indonesia 2Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, University Gadjah Mada, Yogyakarta, 55281, Indonesia 3Department of Animal Breeding and Reproduction, Faculty of Animal Science, University Gadjah Mada, Yogyakarta, 55281, Indonesia 4Education Program in Reproduction and Development, Monash Clinical School, Monash University, Australia Abstract Abstract. Several recent studies theoretically assumed that storage of frozen doses of semen in liquid nitrogen guarantees unlimited sperm functionality. However, several studies conclude that long-term storage causes decreased sperm quality. The purpose of this study is to evaluate the storage duration effect on mitochondrial activity and DNA integrity of Ongole Grade Bull post-Thawed spermatozoa from several Artificial Insemination Centre (AIC) in Indonesia. In this study, Ongole Grade Bull frozen semen that has been stored up to three years were investigated. According to the duration of storage, semen samples were divided into 7 groups (one month, three months, six months, nine months, one year, two years, and three years). This study used 42 straws, with 6 straws for each group were used. Samples were evaluated for sperm motility, viability, membrane integrity, mitochondria activity, and DNA fragmentation. The results show that one-month group sperm motility was (53.80%), viability (72.75%) membrane integrity (76.35%.), mitochondria activity (64.24%), and DNA fragmentation (3.82%). After 3 years of storage, the corresponding values were 39.52%, 41.56%, 48.25%, 49.12%, and 4.62%. Sperm motility and viability remained unaffected up to 6th months of storage, while membrane integrity started decreasing at 3rd month (P<0.05). Mitochondria activity decreased at 9th months (P<0.05). In contrast to the previous results, DNA fragmentation did not increase significantly after 3 years of storage (P>0.05). Thus, post-thaw motility, viability, and membrane integrity of spermatozoa from Ongole Grade Bulls started decreasing at 6th months of storage, mitochondria activity started decreasing after 9th months, while sperm DNA integrity remained unaffected for up to three years of storage. Keywords: Ongole Grade Bull, frozen semen, storage, mitochondria activity, DNA fragmentation, Share Link | Plain Format | Corresponding Author (Kurniawan Dwi Prihantoko)

80	Veterinary Clinic, Reproduction, Pathology	ABS-92
Diagnostic imaging of hip dysplasia in the Kintamani dog FS Murtiningrum1,2- RH Soehartono3- S Widodo4- D Noviana2,3 1 Animal Biomedical Science, Graduate School of IPB University, Bogor, Indonesia 2 Diagnostic Imaging Centre, Animal Teaching Hospital, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia 3 Division of Surgery and Radiology, Department of Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia 4 Division of Internal Medicine, Department of Clinic, Reproduction, and Pathology, Faculty of Veterinary Medicine, IPB University, Bogor, Indonesia Abstract Diagnostic imaging was important modalities for the preliminary diagnosis of hip dysplasia in dogs. The main objective of this study was to determine the radiographic interpretation method in diagnosing hip dysplasia in Kintamani dogs. Hip-extended radiography was the most used position for hip dysplasia and osteoarthritis assessment. A properly positioned hip-extended radiograph was useful as a screening tool for hip dysplasia and for the detection of osteoarthritis but may not adequately represent the degree of hip laxity, therefore the Norberg angle was used to measure the degree of hip dysplasia. The Norberg angle was calculated by measuring the angle between a line that connects the center of the femoral head between the left and right hips and a line that connects the center of the femoral head with the lateral tip of the cranial acetabular rim. A larger angle indicates a deeper acetabulum and a more congruent hip, whereas a smaller angle is consistent with an increased degree of subluxation. Complete knowledge of hip parameters was needed in determining the prognosis of hip dysplasia and supporting efforts to breed the Kintamani dog as the world^s first native Indonesian dog. Keywords: diagnostic imaging, hip dysplasia, Kintamani dog, Norberg angle Share Link | Plain Format | Corresponding Author (Fitria Senja Murtiningrum)

81	Veterinary Clinic, Reproduction, Pathology	ABS-93
Recapilation of Referral cases at Diagnostic Imaging Centre (DIC) Veterinary Teaching Hospital Faculty of Veterinary Medicine IPB University Noor Ihsan Anzary Bahtiar1,2, Arni Diana Fitri1,2,Erly Riska Adistya1,2, Nurul Annisa Tuliman1,2, Hamdika Yendri1, NindyaDwi Utami2, Fitria Senja2, Rili Wahyu Aji2, Frizky Amalia2, Melpa Susanti Purba2, Sitaria Sialagaan2, Abby Pratama2, Deni Noviana1,2 1Veterinary Teaching Hospital, Faculty of veterinary IPB University 2Diagnostic Imaging Center Veterinary Teaching Hospital, Faculty of veterinary IPB University Abstract Teleradiology is the transmission of radiological patient images, such as x-rays, CTs, and MRIs, from one location to another to share studies with other radiologists and physicians. Diagnostic Imaging Center Veterinary Teaching Hospital, Faculty of veterinary IPB University is the only teleradiology service in Indonesia for interpreting diagnostic imaging interpretation such us Xray in animals. This paper aims to describe the recapitulation radiograph referral cases high rate of disease cases that are often interpreted by the DIC (Diagnostic Imaging Centre) team by region based on data from May to July 2020. Based on data on the handling of patients referred to interpretation by DIC from May to July 2020, it shows that the region cases that are often handled are cases of the abdominal region (50%) compared to the thoracic region (28%) and the region of the extremities (22%). Based on these data, it shows that the cases that are often experienced and referred to in carrying out the reading are cases of the liver and intestines. Liver disorders that are often encountered include enlargement or change in the position of the liver. In the intestine organs, abnormalities that are often encountered include the presence of gas masses (gas accumulation), tissue thickening, changes in position, and the presence of foreign bodies. In the process, DIC (Diagnostic Imaging Center) can make it easier for doctors to provide accurate and precise diagnoses, as well as provide advice on further treatment and examinations that can be given. Keywords: Diagnostic, Radiologi, Teleradiology Share Link | Plain Format | Corresponding Author (Noor Ihsan Anzary Bahtiar)

82	Zoonotic Disease and Tropical Disease	ABS-26
Mosquito Community Composition and their Potential as Arbovirus Vectors in South and West Sulawesi Nur Rahma (a*), Isra Wahid (b), Hajar Hasan (c), Arini Ratnasari (a) a) Post Graduate School, Faculty of Medicine, Hasanuddin University *nurrahma5571[at]gmail.com b) Faculty of Medicine, Hasanuddin University c) Department of Parasitology, Faculty of Medicine, Hasanuddin University Abstract The richness of mosquito species and their potential as arbovirus vectors is not yet available, especially in West and South Sulawesi. This research was conducted in Maros, North Toraja, and Pasangkayu with sampling during the dry season and the wet season in 2018. A total of 16939 (44 species, 9 genera) of mosquitoes were collected using Animal Barrier Screen (ABS) and Kelambu Trap (KT) in three locations. The highest species richness is in Pasangkayu (33 species, 8 genera), then North Toraja (27 species, 6 genera), and Maros (25 species, 5 genera). Pasangkayu has 8 special species, while Maros and North Toraja only have 4 special species. Potential arbovirus vectors are found in all locations, Pasangkayu with 82.40% (17 species, 6 genera), then Maros 71.99% (12 species,4 genera), and North Toraja 69.85% (14 species,5 genera). The highest potential arbovirus vectors were Culex tritaenhyorinchus (52.51%), Cx.vishnui (6.91%), Aedes vexan (4.03%), and the rest (15 species) below 4%. Both traps have proven effective in catching mosquitoes, with ABS and KT containing one and eight specific species, respectively. This information is the first data on species richness and potential vectors in the area. Future research will focus on arbovirus detection in collected mosquito species Keywords: Community Composition Measures- Mosquito- Arbovirus- Animal Barrier Screen- Kelambu Trap Share Link | Plain Format | Corresponding Author (Nur Rahma)

83	Zoonotic Disease and Tropical Disease	ABS-30
Mosquito Community in Primate Animal Captivity (Tarsius sp.) and its Potential as Transmitters of Zoonotic Mosquito-Borne Diseases Sarasvathi Cecile (a*), Upik Kesumawati Hadi (a), Uus Saepuloh (b), Sela Septima (b), Syifa Alya (a), Huda Shalahudin Darusman (a,b) a) Faculty of Veterinary Medicine Bogor Agricultural University, Jl. Agatis, Dramaga, Bogor, 16680, Indonesia *sarasvathicecile[at]gmail.com b) Primate Research Center Bogor Agricultural University, Jl. Lodaya II/5, Bogor, 16151, Indonesia Abstract In means of conservation, ectoparasites monitoring and surveillance especially mosquitoes in primate captivity become important. Mosquito is one of the ectoparasites which acts as a vector of various types of zoonotic diseases such as Dengue, Zika, Japanese encephalitis, Rift Valley fever, filariasis and others. This study was aimed to determine the diversity of mosquito species, their fluctuations as well as the potential of mosquitoes as Dengue virus (DENV) and another flaviviridae virus vector around the tarsier captivity in the animal conservation laboratory PSSP IPB. Mosquitoes were collected from February to April 2020 using light traps and sweep net every two hours from 06.00 pm to 06.00 am. Detection of the presence of flavivirus was done using conventional PCR while Dengue virus (DENV) was carried out using Reverse Transcriptase Real-time Polymerase Chain Reaction (RT-qPCR) against Aedes albopictus and Armigeres subalbatus mosquitoes. The results showed that there were 4 species of mosquitoes caught around the tarsier captivity namely Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus and Armigeres foliatus. The dominant mosquito species are Armigeres subalbatus (62.11%) and Aedes albopictus (41.61%). Detection of Aedes albopictus and Armigeres subalbatus for the presence of flaviviridae virus and Dengue virus (DENV) serotypes 1, 2, 3, 4 gave negative results. The presence of mosquitoes that have the potential to carry zoonotic disease around the tarsier captivity in PSSP IPB shows the potential for Mosquito-Borne Diseases to both tarsier and human. Keywords: Dengue virus, mosquito density, tarsius, variety of types, zoonotic disease Share Link | Plain Format | Corresponding Author (Sarasvathi Cecile)

84	Zoonotic Disease and Tropical Disease	ABS-31
Characterization of Vector Community and Collection Density in South and West Sulawesi with host Animal Barrier Screens and Kelambu Trap Nur Rahma (a*), Isra Wahid (b), Hajar Hasan (c), Arini Ratnasari (a) a) Post Graduate School, Faculty of Medicine, Hasanuddin University *nurrahma5571[at]gmail.com b) Faculty of Medicine, Hasanuddin University c) Department of Parasitology, Faculty of Medicine, Hasanuddin University Abstract A total of 7331 mosquitoes were collected in South (Maros and North Toraja) and West Sulawesi (Pasangkayu), using Animal Barrier Screen (ABS) and Kelambu Trap (KT) to characterize the vector community. Mean per night in ABS=128.62 and KT=124.17. Aedes and Culex tended to be trapped in KT (2 locations) than ABS (1 location), whereas Anopheles was the opposite. Armigeres, Lutzia, Mansonia, and Mimomyia are very few and balanced in ABS and KT. Anopheles and Culex are abundant at all three sites, while Aedes and Armigeres are abundant in Maros. Mansonia and Lutzia are mostly in North Toraja, while Mimomyia is only in Pasangkayu. Culex and Anopheles show nearly the same catch pattern in Maros and North Toraja, abundant at 18.00 then decreased and rose again at 20.00 then decreased. Aedes, Armigeres, Lutzia, Mansonia, and Mimomyia tend to decline every hour. Mosquitoes in Pasangkayu tend to be evenly distributed every hour, although there was a slight increase at 23.00. Arbovirus detection in 202 mosquito pools obtained by one pool was positive for Bunyamwera virus (Culex tritaenhyorinchus) in Maros. This latest information will be used as a reference in related research and as a form of arbovirus alertness, so that research on arbovirus detection is still being carried out Keywords: Vector, Collection Density, Mosquito, Trap, Arbovirus Share Link | Plain Format | Corresponding Author (Nur Rahma)

85	Zoonotic Disease and Tropical Disease	ABS-46
ABUNDANCE AND DIVERSITY OF MOSQUITOS IN FILARIASIS ENDEMIC AREA OF BOGOR DISTRICT, WEST JAVA, INDONESIA M. Nirwan (a,b), Upik Kesumawati Hadi (c), Susi Soviana (c), Surachmi Setyaningsih (d), Fadjar Satrija (c) a) Doctoral Program of Parasitology and Medical Entomology, Faculty of Veterinary Medicine, IPB University, Bogor Indonesia. b) Mandala Waluya College of Health Sciences, Kendari, Indonesia c) Division of Parasitology and Medical Entomology, Faculty of Veterinary Medicine IPB University, Bogor Indonesia. d) Division of Medical Microbiology, Faculty of Veterinary Medicine IPB University, Bogor Indonesia. Abstract A research on abundance and diversity of mosquitoes were done in the two filariasis endemic areas in Bogor Regency. The objectives of this study were to determine the abundance and diversity of mosquitos and its importance as vectors of filariasis in two endemic areas in Bogor Regency, including Tamansari Village (rural) and Cimanggis Village (urban). This research was carried out from September 2019 to February 2020. Mosquitoes were collected from 6.00 pm to 6.00 am, twice a month in each village, by using bare leg collection technique. The colected mosquitoes were identified analyzed. The result indicated eight species of mosquitoes in rural area (Tamansari village) i.e Culex quinquifasciatus, Aedes aegypti, Cx vishnui, Cx. tritaeniorhynchus, Armigeres kesseli, Ar. subalbatus, Ae. albopictus, Mansonia annulata. In other hands, there were six species found in urban area (Cimanggis village) i.e. Cx. quinquefasciatus, Ae. aegypti, Ar. kesseli, Ar. subalbatus, Cx. vishnui, Cx. tritaeniorhynchus. Cx. quinquefasciatus found to be most abundance in Tamansari village (90.93%) and also in Cimanggis Village (95.92%), while Ma. annulata was the lowest abundance in Tamansari (0.02%) and Cx. tritaeniorhynchus was the lowest in Cimanggis Village (0.15%). Based on the analysis showed that Cx. quinquefasciatus was the potential vector of filariasis both in rural and urban areas of endemic filariasis of Bogor District, Indonesia. Keywords: Mosquitoes, filariasis endemic, diversity, abundance Share Link | Plain Format | Corresponding Author (Muhammad Nirwan)

86	Zoonotic Disease and Tropical Disease	ABS-52
Identification of Ectoparasites in the Timor Deer (Cervus timorensis) at Kebun Binatang Konservasi Citra Satwa Celebes in South Sulawesi Astri Caturutami Sjahid Universitas Hasanuddin Abstract Parasitic disease that attacks Timor deer can occur if there is an environmental incompatibility. One of the parasitic diseases that have a ^zoonotic^ vector is ectoparasites. The disease can cause discomfort to death in deer. This study aims to identify the diversity of ectoparasite species in Timor deer at the Celebes Animal Image Conservation Zoo, South Sulawesi. This research was conducted in July 2020 with a number of samples taken from 10 Timor deer using diagnostic confirmation carried out in 2 stages, namely examination of clinical and laboratory symptoms. This research shows that the ectoparasites that have been found are the order Diptera, namely Chryzomia bezziana, Musca domestica, Cullicoides, and Hermetia illucens. Keywords: Ectoparasite, Obligate, Facultative, Timor Deer Share Link | Plain Format | Corresponding Author (Astri Caturutami Sjahid)

87	Zoonotic Disease and Tropical Disease	ABS-56
Molecular Detection and Identification of Plasmodium sp Isolated from Cynomolgus Monkeys in Bogor-Indonesia Upik Kesumawati Hadi1, Uus Saepuloh2, Lis Rosmanah2, Susi Soviana1, Huda Shalahudin Darusman1,2 1Faculty of Veterinary Medicine, IPB University, Jl. Agatis, Dramaga, Bogor, 16680, Indonesia 2Primate Research Center, IPB University, Jl. Lodaya II/5, Bogor, 16151, Indonesia Abstract Background: Asian macaques are natural host a number of Plasmodium parasites species. Some of these monkey malaria parasites are able to infect humans and having the potential to causes zoonotic infections. This study was conducted to update the prevalence of monkey malaria parasites in Bogor-Indonesia based on molecular detection and identification, especially in cynomolgus monkeys which has a wide range of geographic distribution and shares extensive habitats with humans. The information is needed to evaluate the status of simian malaria among macaques in Bogor area and to study the potential hazard to human health. Therefore, this updated data will provide adequate information for the implementation of malaria control strategies in the future, as well as to develop the potential malaria vaccine using the monkey as animal model. Methods: Blood samples of 274 cynomolgus monkeys (Macaca fascicularis) were collected and identified by microscopy. The positive blood samples were DNA extracted and analyzed using polymerase chain reaction (PCR) technique to amplify the small subunit ribosomal RNA (SSU rRNA) target gene using consensus primer for genus level of Plasmodium. The PCR positive samples then nucleotide sequenced in commercial sequencing services and the results were analyzed using BioEdit program and aligned using the BLAST program from NCBI. Phylogenetic trees were constructed to see the kinship of the Plasmodium using MEGA 6.0 with Neighbour-Joining (NJ) method. Bootstrapping was performed to assess the robustness of tree topologies by using 500 replicates. Result: Thirty eight out of 274 samples that microscopy detected positive to Plasmodium sp were also positive by PCR technique resulted 1640 bp amplicon. Further analysis using nucleotide sequencing technique confirmed these positive samples were Plasmodium inui with more than 99% nucleotide identity compared to database in genbank. Phylogenetic tree analysis of SSU-rRNA partial gene showed that all o Keywords: Plasmodium inui, small subunit ribosomal RNA, phylogenetic tree Share Link | Plain Format | Corresponding Author (Upik Kesumawati)

88	Zoonotic Disease and Tropical Disease	ABS-62
The adaptability of Aedes aegypti (Linnaeus) larvae coastal and inland ecosystems in South Sulawesi Arini Ratnasari1,b), Nur Rahma2, Arif Rahman Jabal3, Isra Wahid2,a) 1Faculty of Medicine, Postgraduate School, Hasanuddin University Jl. Perintis Kemerdekaan Km.10 Tamalanrea, Makassar 90245, Indonesia 2 Faculty of Medicine, Hasanuddin University Jl. Perintis Kemerdekaan Km.10 Tamalanrea, Makassar 90245, Indonesia 3Dept. Parasitologi, Faculty of Medicine, Palangkaraya University Jl. Yos Sudarso, Jekan Raya, Kalimantan Tengah 74874, Indonesia Abstract Aedes aegypti mosquito is the cause of vector-borne disease. This study aims to look at habitat characteristics, survival, tolerant salinity, and oviposition two ecosystems. This research is an exploratory and experimental study. The study site consisted of coastal and inland area in Makassar City and Maros Regency. Larvae collection carried out at distances of 100 m, 500 m, 1 km and 2 km from the coastline. The collected larvae identified, counted and accompanied pH and salinity measurements. The larvae were breeding to obtain progeny tested at salinity 3,5,10,15 and 20 ppt from each instar stage and the use of ovitraps to see the ability to lay eggs in salinity. Analysis of distribution data, habitat characteristics, survival, salinity torelance, and LC50 test is (SPSS.24) and mapping (ArcGIS 10.5). A total of 2384 larvae were found in the coastal area. Untia, present to 28.2% (n = 1076). Kuri, 34.2% (n = 1308). In the inland region of 1852 individual larvae were found. Biringkanaya, 32.5%, and Marusu, 33.3%. The Larvae Ae. aegypti of the coastal ecosystem are 70% can survive the salinity test treatment, while inland area only 58%. LC50 shows the larval stage in coastal ecosystems has a maximum of 15,64 ppt and a minimum of 10,78 ppt. Meanwhile, the inland area a maximum of 11,09 ppt and a minimum of 9,46 ppt. The results of this study can show the adaptability of Ae.aegypti in two different ecosystems. Therefore, investigation and integrated control in vector control in coastal areas need attention. Keywords: Ae. aegypti, survival, salinity, coastal, inland Share Link | Plain Format | Corresponding Author (Arini Ratnasari)

89	Zoonotic Disease and Tropical Disease	ABS-64
Evaluation of B1 genes nested PCR for detection Toxoplasma gondii: Comparison of six pairs of DNA primers Fitrine Ekawasti (a*), Didik T. Subekti (a), Zul Azmi (a), Muh. Ibrahim Desem (a) a) Balai Besar Penelitian Veteriner * fitrineekawasti[at]gmail.com Abstract Toxoplasmosis, caused by the obligate intracellular protozoan Toxoplasma gondii, is an important zoonosis with medical and veterinary importance worldwide. Diagnosis of toxoplasmosis has been improved by the emergence of molecular technologies to amplify parasite nucleic acids. Among these, polymerase chain reaction (PCR)-based molecular techniques have been useful for the genetic characterization of Toxoplasma gondii. PCR method has already proved to be a useful tool for the diagnosis of toxoplasmosis. This research aimed to evaluate the Primer B1 gens for PCR to diagnose T. gondii. DNA of T. gondii from liquid nitrogen was isolated using the DNAzolTM reagent. Six pairs primer of B1 genes were targeted for the development of partial length and nested PCR. Single and Nested PCR were carried out in a Thermal Cycler (Bio-Rad). PCR was evaluated for the detection of T. gondii. Each primer system used should be evaluated carefully. PCR can successfully be used to detect T. gondii and nested PCR provided higher sensitivity targeting the B1 gene for detection of Toxoplasma gondii. Keywords: Toxoplasmosis, diagnose, PCR, amplification, phylogram Share Link | Plain Format | Corresponding Author (fitrine Ekawasti)

90	Zoonotic Disease and Tropical Disease	ABS-83
IDENTIFICATION OF GASTROINTESTINAL NEMATODE PARASITES IN JAVAN LANGUR (Trachypithecus auratus) FROM THE AREA OF LOMBOK ISLAND Fahrurozi 1), Samsul Hadi 2), Maratun Janah 3), Kunti Tirtasari 4), Candra Dwi Atma 5) and Supriadi*6) 1,2,3,4,5,6 Faculty of Veterinary Medicine, Mandalika University of Education Abstract Javan Langur (Trachypitheces auratus) is one of Indonesia^s endemic primates which a distribution only in Java Island, Bali, and Lombok. Lombok Island has two areas that have a high population of Lutung that is Kerandangan Natural Tourism Park (first area) and Joben Resort SPW II Gunung Rinjani National Park (second area). These areas are quite strategic because they were close to human activities like camping, recreation, and tourism activity. The presence of parasites in Javan Langurs can transmission through water containing nematode worm eggs due to contamination from Javan Langur fecals. The purpose of this study was to identify the species of gastrointestinal nematode worm eggs. This type of research is descriptive with an observational survey study type that aims to collect data on a population in a particular region at a certain time. a total of 30 samples were collected from two areas (20 fecal samples from the first area and 10 fecal samples from the second area. Fecal samples were collected from April 2019 to February 2020. Fecal samples were examined by using the native and sugar flotation methods. Samples were examined under a microscope and parasite identi&#64257-cation was based on the morphology of eggs, and larvae. A total of 4 species eggs nematode parasites was recovered including Oesophagostomum sp, Strongyloides sp, Trichuris sp, and Trychosrtongylus sp. Nematode gastrointestinal parasite from the first area was higher than in the second area. Four species nematode gastrointestinal parasites were identified from the first area and three species from the second area with Trychostrongylus sp was missing in fecal samples from the second area. The result of this study showed that the infection of the parasites was relatively similar and some of the species were potentially transmission to humans (i.e: Strongyloides sp). These parasite data from the Javan Langur were the first report from Lombok Island. Keywords: Javan Langur, nematode parasite and Lombok Island. Share Link | Plain Format | Corresponding Author (Supriadi Supriadi)

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