YAP (Yes-Associated Protein) Dynamics in the Mouse Embryo Transition Phase using Fluorescence Intensity Data
Diah Nugrahani Pristihadi (a), Noer Muhamad Dliyaul Haq (a), Dwi Budiono (a), Vista Budiariati (b), Rini Widyastuti (a,c), Annisa Fithri Lubis (a), Karisma Mardatillah (a), Mokhamad Fahrudin (a), Andriyanto (a), and Arief Boediono (a*)

(a)
Department of Anatomy, Physiology, and Pharmacology, Faculty of Veterinary Medicine, IPB University, Bogor 16680, West Java, Indonesia- (b) Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281, Yogyakarta, Indonesia- (c) Laboratory of Animal Reproduction and Artificial Insemination, Department of Animal Production, Animal Husbandry Faculty, Universitas Padjajaran, Bandung 45363, West Java, Indonesia- *Corresponding author: ab[at]apps.ipb.ac.id

Abstract

In this modern era, the conversion of ^images^ into numerical data was very possible to do. In this study, we have transformed immunofluorescence images into intensity data by utilizing the Zen software. Three sample groups of in vitro cultured mouse embryo throughout the embryonic transition phase (8-cell, morula, and blastocyst) were used to identify the YAP (Yes-associated protein) expression. YAP allegedly was the most important protein in this differentiation process. The prepared samples were observed using the z-stack imaging method by using an LSM confocal. By this method, we are allowed to measure the dynamics of YAP on each cell. The study results showed that the total YAP expressed equally on each cell, but envoy a different manifestation. YAP discovered filling the nuclear, cytoplasmic, or both sides. Intensity analysis of each type of cell showed a non-significant difference among embryo group. In conclusion, cells maintained the YAP equilibrium during the embryonic transitional process.

Keywords: YAP- Mouse embryo- Fluorescence

Topic: Biology and Biomedical Science