Identification of Aeromonas hydrophila Bacteria and its Effect to Histopathology of African Catfish (Clarias gariepinus) Liver
A. Regita Dwi Cahyani, A. Magfira Satya Apada, Dwi Kesuma Sari

Hasanuddin university

Abstract

African Catfish (Clarias gariepinus) is one of fresh water comodity that like by many people because it is easy to breed and has economic value. The purpose of this study was to find out histopathology change of african catfish liver that infected by Aeromonas hydrophila. This research was conducted in May to June 2020. The number of fish samples used in this study was 5 fish. Samples taken were catfish which showed clinical symptoms in the form of lesions on the surface of the skin, swollen abdomen and pale skin color. Identification of A. hydrophila was done by biochemical test using Vitek 2 Compact machine. The result of this test is two out of five sampel are positive infected by A. hydrophila. Histopathological examination was carried out by fixing the liver using 10% neutral buffered formalin (NFB), multilevel alcohol is used for dehydration, embedding using parafifin, cutting and haematoxylin-eosin staining. Analysis of the data used is descriptive qualitative. This research shows the damage or histopathology that occurs in the liver. Damage to the liver in the form of fat degeneration, inflammatory cell infiltration, hemorrhage and necrosis. This damage occurs as a result of the body of catfish infected with A. hydrophila bacteria.

Keywords: Aeromonas hydrophila, African catfish, liver, histopathology

Topic: Marine Biology and Marine Disease