IDENTIFICATION OF Aeromonas hydrophila BACTERIA AND ITS EFFECT ON GILL HISTOLOGY IN LELE DUMBO (Clarias gariepinus)
A. Ananda Sekar Ayu Pertiwi Syakir, A. Magfira Apada, and Dwi Kesuma Sari

Hasanuddin University

Abstract

African catfish (Clarias gariepinus) is one of the most economically important fish species for successful aquaculture. Today the needs of African catfish increase, along with increasing population growth and demand for this catfish. The purpose of this study was to determine the histological features of gills in African catfish that were positively infected by Aeromonas hydrophila. The samples used were 5 African catfish. Sampling was done selectively on fish that showed symptoms of Aeromonas infection that were symptoms of sores such as ulcers on the skin, reddish sores on the mouth, protruding eyes and swollen abdomen. Identification of A. hydrophila bacterium conducted at the Microbiology Laboratory of Makassar City Health Office using a compact vitek-2 machine found that 2 samples of African catfish that were infected with A. hydrophila. Histopathological examination was carried out by gill organ preparations in fixation using 10% neutral buffered formalin (NBF), dehydration using series of alcohol dilution, embedding using paraffin, cutting with a thickness of 4 &#956-m and stained using haematoxylin eosin then observed. Damage or histopathology that occurs in the gills due to bacterial infection A. hydrophila namely fat degeneration, necrosis, hemorrhage and inflammatory cell infiltration.

Keywords: Aeromonas hydrophila, african catfish, gills, histology

Topic: Marine Biology and Marine Disease