Detection of osteoporosis in female ovariohysterectomy and male orchiectomy rats based on blood calcium levels and bone histopathology
Dian Fatmawati (a), Ilham Akbar (b), Irfan Idris (b), Dwi Kesuma Sari (a)

a) Program Studi Kedokteran Hewan, Fakultas Kedokteran Universitas Hasanuddin
b) Fakultas Kedokteran Universitas Hasanuddin

Abstract

The aims of this study is to identify an osteoporosis in ovariohisterectomy female rats and orchiectomy male rats, based on calcium levels and by looking at the bone histopathology. The male whistar rats and female whistar rats were used as a sample in this study. The 24 rats samples were divided into 4 treatment groups, P0, P1, P2 and P3 group. P0 group did not have ovariohysterectomy treatment and P1 group did not have orchiectomy treatment, while P2 group for ovariohysterectomy rats and P3 group for orchiectomy rats. All groups of rat were given standard feed and observed for 6 weeks after orchiectomy and ovariohisterectomy. Rats were placed in the same room with the same temperature, environment and feed. After 6 weeks, blood samples were taken from all rats and bones were taken for histopathological examination.

Keywords: ovariohisterectomy, orchiectomy, osteoporosis

Topic: Animal Laboratories