Page 8 (data 211 to 240 of 298) | Displayed ini 30 data/page
<< PREV 1 2 3 4 5 6 7 8 9 10 NEXT >>

211	Integrated Pest and Disease Management	ABS-189
The Presence of Refugia and Population of Insect Pest in Rice Field Sri Nur Aminah(a*), Andi Nasruddin(a), Nurul Wirid Annisaa(b), Tamrin Abdullah(a), Fatahuddin(a) a)Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jalan Perintis Kemerdekaan KM. 10 Tamalanrea Makassar 90245 b)Magister Student of Phytopathology, Post Graduate Program Bogor Agricultural University, Jalan Raya Dramaga, Babakan Kecamatan Dramaga Bogor 16680, West Java, Indonesia *e-mail: srifirnas[at]gmail.com Abstract Refugia is a type of plant playing roles as a food source and shelter for natural enemies in extreme environmental conditions. Legumes, food plants and flowering plants used as refugia. The purpose research is to study the dominant pest population in bund of rice field planted with refugia. The research was conducted in Tanasitolo village, South Sulawesi from June to November 2017. The study used a Randomized Block Design of five treatments and repeated in four times. The research was started by planting several different plants as refugia in bund of rice fields, namely: P1 = bund planted with maize and soybean- P2 = bund planted with flowering plants- P3 = bund planted with taro and banana- P4 = bund planted with long beans and P5 = bund without plant (control). The results showed that rice fields used various plants as refugia, the insect population was in lowest number. The results showed that Valanga sp. were not found in P1 when the rice was 56 and 63 days after planting (DAP). The number of Nephotettix virescens (0.5 individuals) when the rice was 42 DAP. Leptocorisa acuta has the lowest population at P1 and P3 in the age 84 DAP (10.5 individuals). The conclusion of the experiment, the dominant insect pest population has decreased due to the presence of refugia planted in rice fields. Keywords: rice, refugia,Valanga sp., Nephotettix virescens, Leptocorisa acuta Share Link | Plain Format | Corresponding Author (Sri Nur Aminah)

212	Integrated Pest and Disease Management	ABS-197
Resistance evaluation of sugarcane mutants to Sporisorium scitamineum the causal agent of sugarcane smut disease N Hidayah, K S Wijayanti, M Murianingrum, T Yulianti, B Heliyanto Indonesian Sweetener and Fiber Crops Research Institute Jl Raya Karangploso PO BOX 199 Malang Jawa Timur Indonesia 65152 Abstract Sugarcane is one of important crops as it can produce a broad range of valuable products including food, health, agriculture, and bioplastic. However, it is vulnerable from Sporisorium scitamineum infection. The fungus infects the host through the buds. The disease is characterized by the emergence of whip-like structure at the top of the host plant. The use of resistant varieties is believed to be a proper control method for the disease. Induced mutation is one of breeding program methods that can be considered to acquire resistant varieties of sugarcane to smut disease. In 2018, we did mutation for some sugarcane varieties then in 2019 the mutants were screened for its resistance to smut disease. The inoculation method was conducted by dipping the cane bud into S. scitamineum teliospore suspension for 10 minutes at 30oC. The buds were then planted and maintained in the polybags. The results showed that out of 41 evaluated mutants, 20 of them were resistant, six mutants were moderately susceptible, and 15 mutants were very susceptible to smut disease infection. It seems that we could obtain resistant sugarcane varieties through induced mutation, however the resistance of the cane are still being evaluated during ratoon stage. Keywords: induced mutation, sugarcane smut disease, Sporisorium scitamineum Share Link | Plain Format | Corresponding Author (Nurul Hidayah)

213	Integrated Pest and Disease Management	ABS-202
Inhibition Test Of Betel Extract (Piper betle L.) and Tembelekan (Lantana camara) in Suppressing Anthracnose Growth (Colletotrichum acutatum) Cayenne Chili (Capsicum frutescens) in-vitro Ummul Khalifah, Nur Amin, Muhammad Junaid Department of Plant Pests and Diseases, Hasanuddin University, South Sulawesi, Indonesia Abstract The continuous and excessive use of fungicides will disrupt the balance of the environment and is directly harmful to the health of consumers. The use of plants as botanical pesticides is an ecologically safe control method that has begun to be developed. The purpose of this study was to determine the effectiveness of betel and tembelekan extracts and the effect of the concentrations used in suppressing the growth of Colletotrichum acutatum fungi. Extraction was carried out using the meseration method, then the extract was concentrated using a rotary vaccum evaporator repeatedly until a concentrated solution was obtained. In the test of using treatments with several concentrations, namely K0:0% K1:0,5%, K2:2%, K3:3,5%, K4:5%. Tests were carried out by growing C. acutatum mycelium on PDA media that had been mixed with plant extract solutions according to the treatment concentration. Colletotrichum acutatum mycelium was taken by cutting the PDA which was overgrown with a pure culture of C. acutatum with a media cutter using a cork borer. The results showed that, the higher the concentration of the extract given would reduce the colony diameter of Colletotrichum acutatum and increase the percentage of inhibition. Betel extract at a concentration of 5% had the highest average percentage of inhibition compared to the tembelekan extract, namely 72.45%, while the average percentage of inhibition in the tembelekan extract was lower, namely 37.97%. Keywords: Keywords: Betel and tembelekan extract, Colletotrichum acutatum. Share Link | Plain Format | Corresponding Author (Ummul Khalifah)

214	Integrated Pest and Disease Management	ABS-226
EFFECTIVITY OF VARIOUS EXTRACTS FOR CONTROLLING BLAST DISEASE (Pyricularia oryzae) ON RICE Ade Sugiarti Kumalasari1, Djuniarty MD2, Endah Wijayanti3 University Islamic Of Macassar Abstract The use of natural pesticides for controlling Plant Pest Organisms (OPT). has been done by our ancestors before the era of the green revolution which forced farmers to increase production through the supply of chemical fertilizers and pesticides. Now a days, special attention has been intensified in the development of natural pesticides to reduce the risk of environmental damage from the use of chemical pesticides. It is known that Indonesian is rich in potential biodiversity as a natural pesticide, including gadung tubers, biduri flowers, betel leaves, garlic, clove leaves, soursop leaves and so on. The rice plant is one of the priority food crops of the Ministry of Agriculture at this time, but in its development, it is experiencing problems with the attacks of several major diseases including blast disease (Pyricularia oryzae). Farmers control the disease with the use of chemical pesticides which we are aware of the negative impact on the environment. This study aims to test the effectiveness of the extract which is effective in controlling rice blast disease and can be a recommendation for control at the farmer level. The research included the preparation of P.oryzae isolates, the manufacture of extracts from 4 types of plants, namely gadung tubers, biduri flowers, piper betle leaves and garlic. Field research was carried out in Maros Regency using a randomized block design consisting of six treatments each repeated four times. Ciherang rice varieties are sown first, two weeks after planting (MST) is inoculated with P. oryzae which has been propagated on rice and rice husk media. The application of vegetable extract spraying was carried out at the age of 3 MST, 5 MST and 7 MST. The results showed that the betel leaf extract gave effective results in controlling P. oryzae Keywords: sults in controlling P. oryzae Keywords: Natural pesticides, effectivity, Pyricularia oryzae Share Link | Plain Format | Corresponding Author (ade sugiarti kumalasari)

215	Integrated Pest and Disease Management	ABS-240
THE EXISTENCE OF PESTS AND ITS NATURAL ENEMIES ON VARIOUS PEMATANG PLANTS IN THE RICE FIELD ECOSYSTEM Desriani (a), NurariatyAgus (b*), and Vien Sartika Dewi (b) 1) Magister Program, Department of Plant Pest and disease, Faculty of Agriculture Hasanuddin University Makassar, South Sulawesi 90245, Indonesia. 2) Department of Plant pests and diseases, Faculty of Agriculture Hasanuddin University Makassar, South Sulawesi 90245, Indonesia *) Corresponding author: nurariatyagus[at]gmail.com Abstract The stability of the rice field ecosystem is not only determined by the diversity of community structures but also by the characteristics and interactions of the components. This study aims to determine the diversity of insects pests and its natural enemies in the rice field ecosystem in Marannu village, Mattirobulu district, Pinrang regency, South Sulawesi Province, from July to October 2020. The types of pematang plants were treated, namely cucumbers (Cucumis sativus), corn (Zea mays), long beans (Vigna unguicula) and zinnia flowers (Zinnia elegans). Sampling using a insect net and a pitt-fall trap is then put into a plastic tick and identified. The results showed that there were three parasitoid species from the same order and ten predatory species from six different orders. The dominant parasitoid species was Trichogramma sp., While the largest predator was Lasius niger. In addition, 18 species of pests from six orders were found. Keywords: biodiversity- refugia- parasitoids- predators- pest Share Link | Plain Format | Corresponding Author (Desriani -)

216	Integrated Pest and Disease Management	ABS-243
MORTALITY OF LEPTOCORISA ACUTA IN VARIOUS DOSAGE OF METARHIZIUM ANISOPLIAE Dian Ekawati Sari 1, Moh. Noor Amin Sholeh 1, Hamdayanty 2, Bakhtiar 3 1 Agrotechnology Study Program, Muhammadiyah Sinjai University, Sinjai, Sulawesi Selatan 2 Department of Pest and Plant Diseases, Hasanuddin University, Sulawesi Selatan 3 Food crop and Horticulture Protection Institute, Maros, Sulawesi Selatan. Abstract The main pest of rice plants that attacks in the generative phase and can cause crop failure is Leptocorisa acuta. L. acuta attacks rice plants by sucking rice seeds during the milk ripening phase. This pest control still uses chemical pesticides. The use of chemical compounds at the farm level is very high which has the potential to damage the environment and residues in agricultural products so that control efforts are needed by utilizing the entomopathogenic fungus Metarhizium anisopliae. This study aims to determine the effect of M. anisopliae fungal mortality against L. acuta. The results showed that all treatments with M. anisopliae were significantly different from the control. The highest percentage of mortality was found in the treatment of the fungus M. anisopliae at a dose of 60 grams / l. water was 100% with an average time of death on day 3 after application and an average growth rate of 2.25 after L. acuta died. In 50 gram / l treatment. water and 40 grams / l. of water, respectively, the percentage of mortality was 100% with an average time of death 3.25 and 3.5 days with an average growth fungus rate of 2.5 after L. acuta died. Keywords: Mortality, L. acuta, M. anisopliae Share Link | Plain Format | Corresponding Author (Dian Ekawati Sari)

217	Integrated Pest and Disease Management	ABS-253
Rapid detection of Burkholderia glumae causal agent of grain rot disease in rice seed from Gowa Regency, South Sulawesi using ELISA Andi Alfian Darmawan (a), Tutik Kuswinanti (a*), Asman (a) a) Plant Pest and Disease Department, Agriculture Faculty, Hasanuddin University, Makassar 90245, Indonesia *koeswinanti[at]yahoo.com Abstract Bacterial grain rot in rice plants caused by Burkholderia glumae becomes a serious threat because it is a seed borne pathogen that causes severe damage to rice plantations in several regency at South Sulawesi. This study aimed to rapidly detect of B. glumae causal agent of grain rot disease in rice seed from Gowa Regency, South Sulawesi. Samples were obtained in 10 districts in the main rice producing areas of Gowa regency, South Sulawesi. Sampling using Non Probability Sampling method. 10 Samples that showing grain rot symptoms were detected using Indirect ELISA test with monoclonal antibody. The detection results shown that 9 out of 10 districts are confirmed positive for B. glumae bacteria. Keywords: Bacterial Grain Rot, Burkholderia glumae, ELISA Share Link | Plain Format | Corresponding Author (Andi Alfian Darmawan)

218	Plant Breeding and Biotechnology	ABS-17
Tolerance Response of Ten Chili Genotypes in Limited Watering Condition Redy Gaswanto (a*), Neni Gunaeni (a) a) Indonesian Vegetables Research Institute, Jln. Tangkuban Parahu No. 517, Lembang, Bandung Barat, Indonesia 40391 *redwanto_1[at]yahoo.co.id Abstract Ten chili genotypes of germplasm collection had been evaluated in limited watering condition. The purpose of this research was to obtain genetic material that can be used in breeding program. The study was conducted from February to December 2017 at Indonesian Vegetable Research Institute, Lembang (1,250 m sal). The research used a factorial design with three replications. The first factor was ten chili genotypes and the second factor was the frequency of watering (F0 = daily watering- F1 = watering with interval two days- F2 = watering with interval four days- F3 = watering with interval six days). The seedlings were planted in plastic pots with media soil and sand (1: 2), then be applied the treatments. The population number of each genotype per treatment was 15 plants/replication. The results showed that genotype ANS had better tolerance in limited watering condition compared to other genotypes by some mechanisms, i.e the smallest change in total amount of chlorophyll content but increase in proline content, and reduction in leaf area and stomata number. These result could determine a good strategy for each genotype to be improved as a new variety that tolerance in limited watering condition. Keywords: Capsicum annuum- Limited watering- Pre evaluate- Tolerance Share Link | Plain Format | Corresponding Author (Redy Gaswanto)

219	Plant Breeding and Biotechnology	ABS-275
Fermentation of Arabic Coffee Seeds Coffea arabica using Probiotic Bacteria from domestic chickens Gallus domesticus Alma Amalia Sukriyadi, Dirayah Rauf Husain*, Andi Ilham Latunra, Nurul Iqraini, Riuh Wardhani Department of Biology, Faculty of Mathematics and Natural Science, Hasanuddin University *dirayahrh[at]unhas.ac.id Abstract The Research about fermentation of coffee Coffea arabica using a consortium of probiotic bacteria which is a collection from the microbiology laboratory of the Faculty of Mathematics and Natural Sciences, Hasanuddin University has been carried out on. This study aims to determined the flavor of arabica coffee C. arabica and its chemical composition after the fermentation process. The length of fermentation time was divided into 2 times, namely 24 hours and 36 hours, fermentation using a consortium of probiotic bacteria that had been rejuvenated on coffee peel media for 2 x 24 hours. Organoleptic testing was carried out to see the panelists^ preference for the taste, color and aroma of coffee after fermentation. The results showed that coffee with a 24-hour fermentation time was the most liked by the panelists with the category slightly less acidic taste, slightly black color, and normal aroma. The GC-MS test results showed organic compounds formed after the fermentation process. In the 24-hour control sample there were 14 types of organic compounds, the sample with the addition of a consortium of probiotic bacteria contained 11 types of organic compounds, the 36-hour control sample contained 11 types of organic compounds, and samples with the addition of a consortium of probiotic bacteria contained 13 types of organic compounds. Keywords: Fermentation- Arabic Coffee- Probiotic bacteria- Coffea arabica Share Link | Plain Format | Corresponding Author (Alma Amalia)

220	Plant Breeding and Biotechnology	ABS-278
DETERMINATION OF PHOSPHORUS STATUS ON DUKU (Lansium domesticum) SEEDLING Desi Hernita 1)*, Roedhy Poerwanto 2), Anas D Susila 2) dan Syaiful Anwar 3) 1) Jambi Assessment Institute for Agricultural Technology, Indonesia 2) Department of Agronomi and Horticulture, Bogor Agriculture University, Indonesia 3) Department of Soil Science and Land Resource, Bogor Agriculture University, Indonesia Abstract Symptoms of P deficiency or excessive can be seen mainly in the leaves. The sign can be detected visually and can be done by to identifying P concentration in the each condition. The study of P status was conducted in Jambi Provinces, which was apply on duku seedling that planted in sand culture. The study was conducted in randomized complete block design, with five treatments that consisted of three plants each treatment and in three replications. The treatments consisted of five P levels: 0, 50, 100, 200, and 400 ppm. The results showed that P deficiency symptoms were characterized by stunted growth of seedlings, green-brownish and lusterless discoloration, number of leaves < 4.56 and leaf P concentration < 0.09% (very low nutrient status) and 0.09 &#8804- P < 0.14% (low nutrient status). Sufficienly of P concentration was characterized by normal growth, shiny green leaves, number of leaves 4.56-7.00 and leaf P concentration 0.14 &#8804- P < 0.25% (medium nutrient status). Symptoms of excessive P was showed by stunted growth of seedlings, green leaves with yellow and necrotic spots on the leaf blade, number of leaves < 4.56, P concentrations in the leaf &#8805- 0.25% (high and very high nutrient status). The maximum growth of duku seedling for very low nutrient status was 195 ppm P. Keywords: Phosphorus, seedling,nutrient Share Link | Plain Format | Corresponding Author (Desi Hernita)

221	Plant Breeding and Biotechnology	ABS-281
Potential of Local Orchid As a Source of Material for Genetic Improvement in Central Kalimantan Ronny Yuniar Galingging Assessment Institute for Agricultural Technology (AIAT) of Central Kalimantan - Indonesia Address: Jl. G. Obos KM 5 Palangka Raya , Kalimantan Tengah Abstract The Central Kalimantan Province covers an area of 15.380.000 ha or about 7.93 % of Indonesian area and consists of coastal region open waters and open land area, owns a potential biodiversity such as orchidaceae. The Central Kalimantan consisted of various type of lands e.g. peats soil, acid sulphate and up land. Potential of the orchid^s in Central Kalimantan forest scattered in various forest areas and it is in high diversity various life, form and uses. Method of study: the exploration and colection were conducted at six districts, i.e. Kotawaringin Timur, Kotawaringin Barat, Barito Timur, Barito Selatan, Barito Utara and Murung Raya, from March 2016 until December 2018. The aim of this study was as follows: (1). The exploration process, (2). The ex-situ and in-situ conservation, (3). The characterization process, (4). The documentation process. The results of these activities was there were an ex situ collection of 8 accessions of medicinal plants that can be used as a source of material for genetic improvement. The orchid plant^s conservation could be through in-situ and or ex-situ. The in-situ conservation held by manage the forest area as the natural habitat of the material for genetic improvement, while the ex-situ conservation held outside of the native habitats. Wise utilization and research activities are important in conservation of the material for genetic improvement Central Kalimantan. Keywords: orchid, conservation Share Link | Plain Format | Corresponding Author (Ronny Yuniar Galingging)

222	Plant Breeding and Biotechnology	ABS-30
Agronomic characteristics of elite soybean lines and the response to pod shattering Ayda Krisnawati, Yuliantoro Baliadi, Eriyanto Yusnawan, Andy Wijanarko, M. Muchlish Adie Indonesian Legume and Tuber Crops Research Institute (ILETRI) Abstract The soybean productivity can be increased simultaneously by improving the genetic potential and saving the yield losses due to pod shattering. The research aimed to evaluate the agronomic characters of 14 soybean elite lines and two check varieties and to identify their resistance to pod shattering. The experiment was conducted in two stages, i.e. field, and laboratory research. The field experiment was conducted in Mojokerto (East Java, Indonesia) from February to May 2019, and arranged in a randomized block design using 16 soybean genotypes with four replications. When the plants were in the R8 phase, five plants were randomly taken from each genotype to be tested their resistance to pod shattering using the oven-dry method in the laboratory of ILETRI, Malang. The agronomic characters of days to flowering, days to maturity, 100 seed weight, and seed yield were significantly different between genotypes, meanwhile, the other agronomic characters (plant height, number of nodes, number of branches, number of empty pods, number of filled pods, seed weight per plant) were not significantly different. The range of seed yield of 14 elite lines was 2.76 - 3.14 t/ha, and the check varieties of Anjasmoro and Gema were 2.56 t/ha and 2.76 t/ha, respectively. All elite lines have large seed size but medium maturity. The shattering evaluation obtained two resistant lines and twelve highly susceptible lines to pod shattering. Two high yielded elite lines (3.14 t/ha and 3.11 t/ha, respectively) showed a highly susceptible reaction to pod shattering. The two resistant elite lines produced a yield of 3.0 t/ha and 2.97 t/ha, respectively. Those four elite lines could be recommended for varietal development, but with the implication that the high yielded lines but susceptible to shattering need to be harvested immediately after maturity. On the contrary, it was possible to delay harvest for the resistant elite lines without causing significant yield losses. Keywords: harvest delay, high yield, yield losses, improved variety Share Link | Plain Format | Corresponding Author (Ayda Krisnawati)

223	Plant Breeding and Biotechnology	ABS-40
Propagation of Mycorrhizal Fungi at Various Combinations of Organic Culture Media Muhammad Akhsan Akib(a*), Andi Nuddin(a), Retno Prayudyaningsih(b), Tutik Kuswinanti(c), Syatriyanty Andi Syaiful(c), Sarjiya Antonius(d). a) Universitas Muhammadiyah Parepare. South Sulawesi, 91131. Indonesia. *akhsanbagus[at]yahoo.co.id b)Environment and Forestry Research and Development Institute of Makassar. South Sulawesi, 90243, Indonesia. c)Hasanuddin University. South Sulawesi, 90245, Indonesia. d)Indonesian Institute of Science. Jakarta, 12710, Indonesia. Abstract Utilization of arbuscular mycorrhizal spores carrier media which has heavyweight, not optimal utilization of organic material as arbuscular mycorrhiza inoculant carrier, and founded of arbuscular mycorrhizal spores which have wide adaptability and tolerance on land contaminated with heavy metals are the basis for implementation of this research. The aim of this research was to determine combination of organic culture media which is good for increasing the abundance and diameter of indigenous Gigaspora sp. This research was carried out at Universitas Muhammadiyah Parepare, and Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Makassar. The research was compiled using a completely randomized design. The combination treatment of organic culture media used was a combination of rice husk charcoal, sand, zeolite- rice husk charcoal, sand, sawdust- rice husk charcoal, sand, cocopeat- rice husk charcoal, sand, paddy soil- rice husk charcoal, sand, cold larva. The results showed that combination of rice husk charcoal, sand, cocopeat- and combination of rice husk charcoal, sand, paddy soil gives the best results on growth and development of Gigaspora spores. Combination of rice husk charcoal, sand, and cocopeat, can be recommended as media an effective, efficient, and inexpensive spore carrier medium, but should be used after decomposing into compost. Keywords: carrier media-rice husk charcoal-cocopeat-endomycorrhizal-sawdust. Share Link | Plain Format | Corresponding Author (Muh. Akhsan Akib)

224	Plant Breeding and Biotechnology	ABS-296
Development of Image-Based Phenotyping for Selection Characters of Rice Adaptability on The Seedling Salinity Screening Muhammad Fuad Anshori (a*), Muh Farid (a), Nasaruddin (a), Yunus Musa (a), Hari Iswoyo (a), Andi Isti Sakinah (b), Muh Arifuddin (c), Adinda Asri Laraswati (b) a)Agronomy Department, Agriculture Faculty, Hasanuddin University *fuad.pbt15[at]gmail.com b)Agriculture System Graduate Scholar, Graduate School, Hasanuddin University c)Agrotechnology Graduate Scholar, Graduate School, Hasanuddin University Abstract Development of adaptability rice under salinity stress needs effective and selective methods in the screening process. The seedling screening method is a general method used in salinity screening. However, this screening method often uses conventional observation in its screening process. This observation is rated that has a high error level. Therefore, the development of a digital approach through image-based phenotyping expected could minimize the error in the adaptability screening. This study was designed with a nested randomized complete group design, where replications were nested in a stressful environment. The stressed environment in this study was normal (0 mM NaCl) and salinity stress (120 mM NaCl). The genotype used consisted of 8 genotypes which were repeated three times. The number of characters observed was nine image-based phenotyping. The results of this study showed that green percentage, the 3rd leaf length, and total area were the selection characters of image-based phenotyping under seedling salinity screening. Besides that, the used adaptability index in salinity screening became a good approach in considered and distinguished tolerance responses among varieties, especially to Pokkali (tolerant control variety) and IR 29 (sensitive control variety). Based on this study, application of image-based phenotyping recommended in the screening process of line adaptability under salinity stress. Keywords: Adaptability, Image processing, Hydroponic culture, Pokkali, Salinity stress Share Link | Plain Format | Corresponding Author (Muhammad Fuad Anshori)

225	Plant Breeding and Biotechnology	ABS-299
The growing of taro Colocasia esculenta Var. Antiquorum plantlet in several media during acclimatization stage M Tuwo1, E Tambaru1 1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Hasanuddin, Makassar, South Sulawesi, 90245 Abstract Japanese taro or satoimo Colocasia esculenta (L.) Schott var. Antiquorum, is widely consumed in Japan thus increasing exports. Plant seedlings can be obtained in large quantities and in a relatively short time through the propagation method of plant tissue culture. Final stage of plant tissue culture is acclimatization. Growing media and planting techniques are important factors in the acclimatization process. The ideal planting medium can be obtained from a combination of organic and inorganic materials. This study aims to obtain optimal acclimatization media for the growth of taro plants. The planting material used 24 weeks old taro plantlets. The plantlets were washed with tap water and soaked in distilled water + fungicide and washed with sterile water. The plantlets were planting in plastic cups with the different substrate mixture namely (P1): Soil: Manure: Rice Husks (1:1:1)- (P2) Soil: Manure: Cocopeat (1:1:2)- (P3) Rice Husk: Sand (1:1)- (P4) Nutrient Film Technique (NFT) Hydroponics System. They were watered daily and fertilized. Survival percentage, plantlet height and number of leaves were recorded for 12 weeks. Data were analyzed using ANOVA and if there is a significant effect of treatment, a further test of the least significant difference (LSD) is carried out. The optimal acclimatization medium for acclimatization was obtained in (P1) Soil: Manure: Rice Husks (1:1:1) treatment with a percentage of survival of 62% with an average plant height and number of leaves 13.3 cm and 4.6 respectively. Keywords: acclimatization, cocopeat, NFT system, rice husk, satoimo Share Link | Plain Format | Corresponding Author (Mustika Tuwo)

226	Plant Breeding and Biotechnology	ABS-44
Sterility of 20 F-1 Genotypes Derived from Hybridization of Several Chili^s Lines with M1 Male Sterile Redy Gaswanto1*) 1) Indonesian Vegetables Research Institute, Jln. Tangkuban Parahu No. 517, Lembang, Bandung Barat, Indonesia 40391 *)Correspondence E-mail: redwanto_1[at]yahoo.co.id Abstract Some open pollinated (OP) chili varieties seed already had been irradiated successfully by gamma rays (400-600 Gy) to obtain four plant individuals M1 male sterile. These sterile individuals could be used as a breeding material for F1 chili^s cyto-genic male sterile (CGMS) hybrid. The purposes was to evaluate sterility trait of 20 F1 genotypes derived from hybridization of several chili^s lines with four M1 male sterile individuals until could be categorized as candidate lines of male sterile, maintainer, and restorer. The research conducted at Indonesian Vegetables Research Institute, Lembang (1,250 m sal) from April to December 2018. The research used Randomized Complete Block Design (RCBD) with three replications. The population of each hybridization genotype was 10 plants/replication. Sterility trait evaluation was observed by fruitful ability of various flower^s grouping positions (under, middle, upper) on each hybridization genotype plant. The research result showed that there were two chili genotypes categorized as sterile lines and five genotypes as fertile lines. Male parent of sterile genotypes was a maintainer line, whereas male parent of fertile genotypes could be a restorer line candidate. Keywords: cytoplasmic male sterile, maintainer, restorer, F-1 hybrid, gamma rays irradiation Share Link | Plain Format | Corresponding Author (Redy Gaswanto)

227	Plant Breeding and Biotechnology	ABS-48
Rice grain quality evaluation of some promising lines for swampy rice and upland rice Dody D. Handoko, Aris Hairmansis, Rini Hermanasari, Indrastuti A. Rumanti, Untung Susanto, Trias Sitaresmi, Yudhistira Nugraha Indonesian Center for Rice Research, Sukamandi, Subang, Jawa Barat, Indonesia Abstract The research aimed to determine the rice grain quality of some promising lines from the advanced breeding generation of swampy rice and upland rice. The research material was 43 rough rice samples from the multilocation test of swampy rice (16 rice lines, 3 variety checks) and upland rice (20 rice lines, 4 variety checks) harvested in 2018. All rough rice samples were analyzed the rough rice physical quality, milled rice physical quality, milling quality, physicochemical quality, and organoleptic quality (scoring test and hedonic test). The results showed that in general, the promising lines of swampy rice had higher values of unpolished rice yield, polished rice yield, head rice percentage, and protein content than those of upland rice. In addition, the promising lines of swampy rice had lower empty rough rice and immature+ chalky grain than those of upland rice lines. Among the promising lines of swampy rice, B13926E-KA-43, B13931E-KA-33, and BP30400F-KA-5 had the highest head rice percentage, unpolished rice yield, and polished rice yield, whereas, among the promising lines of upland rice, B15392D-KR-12, B15511D-KR-20, and B15514D-KR-5 had the highest head rice percentage, unpolished rice yield, and polished rice yield. Keywords: rice grain quality, promising rice lines, swampy rice, upland rice Share Link | Plain Format | Corresponding Author (Dody Dwi Handoko)

228	Plant Breeding and Biotechnology	ABS-64
Selection of several hybrid maize (Zea mays L.) genotypes under low nitrogen condition Azmi Nur Karimah Amas (a), Muhammad Yusril Hardiansyah (a), Yunus Musa (a) and Andi Rusdayani Amin (a) (a) Department of Agronomy, Agrotechnology Study Program, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia Abstract Nitrogen is an important nutrient that is needed by maize plants in all growth stages. Low soil N nutrient content is a limiting factor in efforts to increase the maize production. Hybrid maize varieties are generally very responsive to fertilization and have high yields in optimal environments in contrast to abiotic stress conditions such as low nitrogen. Therefore, it is necessary to develop hybrid maize with low N tolerance by genotyping at low N fertilization conditions. The research aims to obtain a genotypes of low nitrogen tolerant hybrid maize with high production and to determine which characters have high heritability values. The research was carried out in the Experimental Garden of the Cereals Plant Research Institute, Bajeng Subdistrict, Gowa Regency, South Sulawesi Province. The research was arranged in a Split Plot Design with the main plot is a dose of nitrogen (0 kg N/ha, 100 kg N/ha, 200 kg N/ha) while subplots were genotypes hybrid maize consist of 12 genotypes and 3 comparative varieties (Nasa 29, Bisi 18, and Jakarin 1). The results showed that the hybrid nitrogen tolerant genotypes of low nitrogen with high production were AVLN 83-2 x AVLN 124-9 genotypes in the treatment without nitrogen fertilization and AVLN 83-2 x AVLN 32-8, AVLN 83-2 x AVLN 124-4 , AVLN 83-2 x AVLN 124-9, AVLN 83-2 x AVLN 100-1, AVLN 122-2 x AVLN 124-9, AVLN 122-2 x AVLN 100-1, and AVLN 118-7 x AVLN 124- 9 at a nitrogen dose of 100 kg/ha. The conclusion of this research is that there are several genotypes that are tolerant of low nitrogen and there are several characters with high heritability values such as ear weight, ear diameter, ear length, 1000 seed weight, and productivity. Keywords: Selection, hybrid maize, nitrogen. Share Link | Plain Format | Corresponding Author (Azmi Nur Karimah Amas)

229	Plant Breeding and Biotechnology	ABS-103
Identification of Fungi associated with the soil area of natural forest and post-mining area of PT Vale Indonesia S W Jufri, A Arif, I Iswanto, S H Larekeng Faculty of forestry, Universitas Hasanuddin Abstract This study aimed to identify fungi that are able to inform the microbes associated with post-mining forest soil of PT. Vale Indonesia. Here, we used the descriptive method by noting and drawing the observed phenomena. Sample collection in the field was carried out by collecting 500 g of soil per spot (three repetitions). The samples were from the rooting area or rhizosphere at 10-30 cm of depth in natural forest and reclamation areas. Isolating for growing the microbes was using the dilution technique. The purification of the obtained isolates was performed by inoculating in the PDA media with the point method, then incubated for +- five days at 27 oC and their growth was observed. Characterization of microscopic fungi isolates was identified based on the characteristics of their morphological structures. Initial research results of fungi soil samples from reclamation and natural forest areas obtained 11 rhizosphere fungi isolates. Based on the fungi colonies^ color at the top and bottom, they had various colors and different textures. The color was dominated by greenish and whitish, but some isolates had white, brown, cream, gray, yellowish, whitish, greenish, and spots. The texture was dominated by velvet, but some isolates had fine cotton and coarse cotton textures that were assumed as Aspergillus niger, Aspergillus sp, Fusarium oxysporum, Penicillium sp., Trichoderma harzianum, and Trichoderma viride. This study will be followed by validating the type and function of each isolate. Keywords: Fungi, post-mining, identification Share Link | Plain Format | Corresponding Author (Siti Halimah Larekeng)

230	Plant Breeding and Biotechnology	ABS-109
Identification of Plant Growth Promoting Rhizobacteria in Rhizosphere of Bamboo Thorns with Gram Methylene Blue and Lugol Staining Muhammad Yusril Hardiansyah (a*), Yunus Musa (a), Abdul Mollah Jaya (a) a) Department of Agronomy, Agrotechnology Study Program, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia Abstract Plant Growth Promoting Rhizobacteria is a group of mirorooganisms that can increase plant growth and yield through reactions that occur in the soil, however, the large number of microorganisms contained in the PGPR rhizosphere of bamboo thorns makes it difficult to know which bacteria are the most dominant and most active in influencing plants. Generally, gram staining of bacteria aims to facilitate the observation of bacterial morphology with the aid of a microscope. Bacteria are generally colorless and almost invisible due to the lack of contrast with the water in which they may be present. Staining using methylene blue and lugol is generally needed to see the bacteria clearly. This study aims to determine the effect of using the gram stain test method with methylene blue and lugol in identifying PGPR bacteria from rhizosphere of bamboo thorn. This research was conducted at the Laboratory of Biological Fertilizers and Potential Microbes, Faculty of Agriculture, Hasanuddin University in October-December 2019. The research methods included the manufacture of microorganism planting media, pouring media, planting bacteria using the scatter and scratch method, and the gram reaction test using methylene blue and lugol. The results obtained showed that the use of the gram test with methylene blue and lugol resulted in a blue color which indicated the dominance of the genus Pseudomonas, while the pink/purple color indicated the dominance of the genus Bacillus. Changes in the color of the bacteria to pink/dark purple, the bacteria were gram negative and belong to the Pseudomonas group and there was a change in blue, so the bacteria were gram-positive and were group of Bacillus. Therefore, the use of the gram test with methylene blue and lugol has an optimal effect in detecting microorganisms especially in identifying PGPR. Keywords: Gram Test, Lugol, Methylen Blue, Microorganisms, Thorn Bamboo Share Link | Plain Format | Corresponding Author (Muhammad Yusril Hardiansyah)

231	Plant Breeding and Biotechnology	ABS-112
Testing Viability of Burik Sinjai Local Rice Seeds with the Using Top of Paper Method Dian yustisia1, Kahar Mustari2, Tutik Kuswinanti3, Amir Jassi4 Hasanuddin University Abstract The development of seed technology is the first step in plant cultivation activities. One of the important seed activities is testing the germination and growth strength of a seed. Dotted rice is a potential local variety because it has a distinctive aroma and delicious taste. This variety is also resistant to pests and diseases. Along with the times, introduced rice is increasingly diverse and superior, local farmers are starting to leave this local variety, besides because it has a long harvest period, also because some farmers still use conventional methods so that this local variety is increasingly rare in the area origin. The results of this study indicate that the germination of local Burik rice seeds using the endophytic fungal metabolite filtrate technology on paper test method gave the best results on the viability of local Burik rice seeds. Keywords: Local seeds, germination, endophytic fungi Share Link | Plain Format | Corresponding Author (dian yustisia)

232	Plant Breeding and Biotechnology	ABS-133
The dynamics of physiological properties of ebony (Diospyros celebica bakh) based on crown position and altitude S A Paembonan1), S H Larengkeng2), S Millang1) 1) Silviculture Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. 2) Forestry Biotechnology Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. Abstract Knowledge of the physiological properties of tree species is one of the basic requirements in determining the prescription silvicultural treatment of stands in nature. This study aims to analyze the physiological characteristics of the species of ebony (Diospyros celebica Bakh), as one of the potential endemic species of Sulawesi, based on leaf position in the crown and differences in altitude. Leaf sampling locations were in the Hasanuddin University Educational Forest (507 m asl) and Makassar city (16 m asl). The research variables consisted of: number of stomata, size of stomata, leaf stomata index, and leaf chlorophyll content. The results showed that the highest number of stomata was found in the leaves exposed to the sunlights with 52,900 stomata/mm2 compared to 29,800 stomata / mm2 in shaded leaves. Likewise, the highest chlorophyll content was found in leaves exposed to the sunlights of 0.043 mg/L and shaded leaves of 0.034 mg /L. The difference occurs based on differences in altitude as well. The number of stomata in the lower area was 42,200 stomata/mm2, while in the high area was 57,600 stomata / mm2. The chlorophyll content in the low area was 0.045 mg/L and in the high area it was 0.041.mg/L. The higher a place, the more the number of stomata increases, this is inversely proportional to the size of the stomata and the stomata index, while the number of chlorophylls is not significantly different. Keywords: Ebony (Diospyros celebica Bakh), leaf stomata, chlorophyll content, altitude, silvicultural prescription Share Link | Plain Format | Corresponding Author (Samuel Arung Paembonan)

233	Plant Breeding and Biotechnology	ABS-140
Amplification of rbcl and matK genome regions in bamboo plant species: preliminaries studies Jeni Oktavina Kamben Patintingan, Siti Halimah Larekeng, Muh. Restu Faculty of Forestry, Universitas Hasanuddin Abstract bamboo is widely used as a raw material for creative industries with economic value. About 157 species or 10% types of bamboo growing in Indonesia and 50% are endemic to Indonesia. Morphological and molecular identifications are required as the basic information for the hybridization and selection strategies. Morphological identification is performed based on morphological characters. Molecular identification can be assessed using DNA barcoding. According to the CBOL plant group, matK and rbcL are the best barcode combination for plants. In this research, we selected five matK and two rbcL primers to obtain primers that are able to amplify bamboo DNA from 2nd regional of forest seed/seedling office. Only three out of seven primers, matK 4, matK 5, and rbcL 1, could amplify the evaluated DNAs. matK 2, matK 7, matK 8, and rbcL 4 did not produce any PCR products. rbcL 1 and matK5 generated PCR amplification products, which were 700 bp in size. The size of PCR products using matK4 was 900bp. This primer amplified 15 of 16 the evaluated samples. Keywords: bamboo, DNA barcoding, matK, rbcL Share Link | Plain Format | Corresponding Author (Siti Halimah Larekeng)

234	Plant Breeding and Biotechnology	ABS-159
THE EFFECT OF EXPLANT SIZE AND ADDITION of ANTIVIRAL RIBAVIRIN ON PROIFERATION OF MERISTEMATIC POTATOES (Solanum tuberosum L) Asih K. Karjadi and Nurmalita Waluyo Indonesia Vegetable Research Institute Abstract The potatoes (Solanum tuberosum L) are belongs to genus solanacearum which is propagated vegetatively through tubers or cuttings. Viral systemic diseases is an important that can be carried on the seed, with unconventional techniques of tissue culture combined with chemotherapy can help to eliminated systemic diseases, especially viruses. The research activity was carried out in the tissue culture laboratory of Indonesian Vegetable Research Institute (IVEGRI) on April untill November 2018. This activity aimed to observed the application of antiviral Ribavirin on MS medium to the growth and development of meristematic tissue (E1), shoot tip (E2), (2), antiviral Ribavirin consentration (0- 5- 10 mg/l) with MS supplement GA3 0.15 mg/l , kinetin 0.1 mg/l , CaP 2 mg/l , Myo inositol 100 mg/l , coconut water 100 ml/l ,sucrose 30 g/l , agar 6.5 g/l , pH 5.7 (3) The varieties were Granola, Median and Atlantic .The results of experiment (1) percentage growth and development meristematic on 4 WAP 50 - 80%, contamination 20 - 50%. (2) percentage of normal growth 40 - 50 , average number of shoot 0.25 - 0.95, the number of nodes 0.35 - 2.50, roots 0.20 - 0.93. (3) visual observation of antiviral Ribavirin on MS media visually influences growth and explant shoot tip growth better than meristem of the three variety . Keywords: Potato ( Solanum tuberosum L)- Antiviral Ribavirin - Meristematic tissue Share Link | Plain Format | Corresponding Author (Asih Kartasih Karjadi)

235	Plant Breeding and Biotechnology	ABS-218
Changes in agronomic tolerance of several genotypes of maize to waterlogging Ahmad Muliadi1, Yustisia2, Amin Nur3, and Muhammad Azrai1 1 Indonesian Cereals Research Institute 2 South Sumatra Assessment Institute for Agricultural Technology 3Gorontalo Assessment Institute for Agricultural Technology Abstract Waterlogging is a major factor in reducing crop yields. To increase plant productivity in temporarily waterlogged soils, the development of waterlogging tolerant lines is needed. This study aims to determine the genotype of maize that is tolerant of waterlogging and morphological characters that can be used for selection criteria. The experiment was carried out in greenhouse of Maros experimental farm. A total of 40 maize genotypes and 2 check varieties, namely MGold and Bima 19, were tested using a randomized block design, 3 replications. Screening was carried out in 2 conditions, namely waterlogged and normal conditions. Based on the waterlogging sensitivity index (WSI), the WSI value was different for each variable. The results of the main component analysis showed that the variables that had a major influence on the diversity of tolerance of maize genotypes to waterlogging stress were plant height and root length so that they could be used as selection characters for waterlogging stress. Genotypes classified as tolerant are G3, G3, G4, G5, G8, and G13. Keywords: Keywords: tolerance, genotype, maize, waterlogging Share Link | Plain Format | Corresponding Author (Ahmad Muliadi)

236	Plant Breeding and Biotechnology	ABS-242
Effect of the fermentation process using a consortium of probiotic bacteria on the flavor of arabica coffee Coffea arabica Nurul Iqraini, Dirayah Rauf Husain*, Andi Ilham Latunra, Alma Amalia, Riuh Wardhani Department of Biology, Faculty of Mathematics and Natural Science, Hasanuddin University *dirayahrh[at]unhas.ac.id Abstract The research about the fermentation of coffee Coffea arabica using a consortium of probiotic bacteria has been carried out. This study aims to determined the flavor of arabica coffee C. arabica and its chemical composition after the fermentation process. The fermentation time was divided into 3 times, consists of 24 hours, 36 hours, and 48 hours using a container measuring 10 L, each containing 1.5 kg of coffee and 75 mL of rejuvenated probiotic bacteria cultures. Organoleptic testing was carried out to see the panelists preference for the taste and aroma of coffee after fermentation. The results showed that coffee with a fermentation time of 48 hours had the best taste and aroma. The GC-MS test results showed several compounds were detected after the fermentation process including furan, phenol, propanoate acid, quinic acid, purine, palmitic acid, pyrol, ascorbic acid, linoleic acid, stearic acid, oleic acid, amines, piran, purines, aldehydes, vitamin E, benzadrex, hexene, tocophenols and arachidic acid. Keywords: Fermentation- arabica coffee- consortium bacteria- probiotic bacteria Share Link | Plain Format | Corresponding Author (Nurul Iqraini D)

237	Smart Farming and Agricultural Engineering	ABS-2
Usability Testing of an Android Application of Tractor Distribution Information System (GIS Tractor) Andi B Kresna (a*), M Achmad (b), Iqbal(b) and A P Hanifa (cd) a*) Batangkaluku Agricultural Training Center, Agricultural Human Resource Extension and Development Agency, Gowa South Sulawesi, Indonesia b) Agricultural Engineering Program, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia c) South Sulawesi Assessment Institute for Agricultural Technology, Makassar, Indonesia d) School of Agriculture and Environment, Massey University, Palmerston North, New Zealand Abstract Lack of information in machinery distribution, including tractors, is one of challenge in promoting mechanization and supporting information disclosure. Previous research has developed an android application, namely GIS Tractor Maros as an improved information system of tractor distribution in Maros. As a new android application, the respond and acceptability of users are essential for improvement. This study was conducted to measure the acceptability of GIS Tractor based on USE questionnaire evaluation (Usefulness, Satisfaction, and Ease of Use). Online questionnaires are used as a research instrument which constructed as five-point Likert rating scales to rate a set of statements. Result showed that GIS Tractor had met the five aspects of usability testing. The use of GIS tractor android application can assist the user to locate the distribution of tractor within Maros Regency faster than the manual system. Keywords: android, distribution, GIS, tractor, usability Share Link | Plain Format | Corresponding Author (Arini Putri Hanifa)

238	Smart Farming and Agricultural Engineering	ABS-279
Technology intervention to unleash the flavor potential of arabica coffee from Sulawesi highland Salengke, S. (a*), Hasizah, A. (b), Reta, R. (c), and Mochtar, A.A. (d) a) Agricultural Engineering Study Program, Hasanuddin University, Indonesia * ssalengke[at]yahoo.co.id b) Food Science and Technology Study Program, Hasanuddin University, Indonesia c) Agroindustry Study Program, State Agricultural Polytechnic Pangkep, Indonesia d) Mechanical Engineering Department, Hasanuddin University, Indonesia Abstract Coffee is arguably the most popular drink in the world after water and it is estimated that global coffee consumption exceeds 1.5 billion cups every day. Today, coffee is not only consumed for pleasure but it is also used as a catalyst for social gatherings and networking. This is due to the fact that coffee can provide pleasurable taste and aroma. In order to optimize these organoleptic properties, technology interventions during postharvest processing are needed to unleash the potential flavor in coffee beans. This study was done to address this issue, especially for improving flavor and aroma potential of arabica coffee beans from Enrekang and Tanah Toraja in the highland of Sulawesi. Technology interventions in the form of fermentation (wet process) and drying were carried out and flavor notes and aroma were assessed through cup test. The results indicated that cup test scores can be improved and different flavor notes can be generated through different processing conditions. Keywords: Sulawesi highlands- arabica coffee- fermentation- organoleptic properties Share Link | Plain Format | Corresponding Author (Salengke Salengke)

239	Smart Farming and Agricultural Engineering	ABS-284
Aplication of aqua crop model in simulation of irrigation management on paddy field in Tanabangka village, Gowa district B Rasyid, A Febrianti RSA, and S Laban Dept. of Soil Science, Fac. of Agriculture, Hasanuddin University- Makassar. Abstract Water management in rice production is one of the important factor to ensure of rice cultivation successfully. The purpose of this study is application of Aqua Crop model to simulate on how much irrigation water and prediction of water supply differentiation in the area of study during rice cultivation period. This research was conducted in Tanabangka village, Gowa district, South Sulawesi. The stages of this research include: (1) collecting climate data, (2) calculation of water debit by the load method, (3) soil sample analysis in the laboratory (4) analysis of data using open source Aqua Crop Software (V. 6.1). The results of the analysis using the Aqua Crop model show a high amount of water supply of 1157.01 mm/season with an estimated ETo average of 66.15 mm/season. Furthermore, in plot 3 the highest value of water availability is 33.35 g/g, with the amount of water given at 766.18 mm/season, and production 6.9 tons/ha. The Aqua Crop simulation for the management of irrigation water can be used well by taking into account the influence of environmental factors on rice production. Keywords: Aqua Crop model, paddy field, water management, rice production Share Link | Plain Format | Corresponding Author (Burhanuddin Rasyid)

240	Smart Farming and Agricultural Engineering	ABS-293
Design and Building Gratter and Stirrer Mechine Sago Pith Aliah Rahman1, Ahmad A. Latuponu2, Agung K. Henaulu3 Universitas Darussalam Ambon Abstract Abstract. This study aims to design a sago pith grating and stirring machine. Sago (Metroxylon sp) is a carbohydrate-producing plant and has become one of the staple foods of people in Maluku. The main population of sago plants in Maluku is scattered in the West Seram, East Seram, Central Maluku and Buru Districts. So far, sago flour is only processed as a staple food substitute for rice which is cultivated on a household scale, while towards industries that use technology it has not been done optimally. Therefore, sago development needs to be supported by several factors and one of them is technology, which with a touch of technology in sago processing will increase the income of sago farmers and local revenue. In the Maluku region, it takes at least 3 workers to process sago, where they have their respective duties. Smoking (dredging) sago stalks still uses nani that is moved by humans. In terms of efficiency, it is very time consuming if you have to cut 1 sago tree, it takes about 1-3 weeks. The equipment specification is 47.5 cm long, 31 cm wide and 100 cm high. Using a gasoline motor as propulsion. Keywords: Mechine Design, Sago, Sago Pith Share Link | Plain Format | Corresponding Author (Agung K. Henaulu)

Page 8 (data 211 to 240 of 298) | Displayed ini 30 data/page
<< PREV 1 2 3 4 5 6 7 8 9 10 NEXT >>

Powered By Konfrenzi Ultimate 1.832L-Build7 © 2007-2025 All Rights Reserved