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THE EFFECT OF EXPLANT SIZE AND ADDITION of ANTIVIRAL RIBAVIRIN ON PROIFERATION OF MERISTEMATIC POTATOES (Solanum tuberosum L)
Asih K. Karjadi and Nurmalita Waluyo

Indonesia Vegetable Research Institute


Abstract

The potatoes (Solanum tuberosum L) are belongs to genus solanacearum which is propagated vegetatively through tubers or cuttings. Viral systemic diseases is an important that can be carried on the seed, with unconventional techniques of tissue culture combined with chemotherapy can help to eliminated systemic diseases, especially viruses. The research activity was carried out in the tissue culture laboratory of Indonesian Vegetable Research Institute (IVEGRI) on April untill November 2018. This activity aimed to observed the application of antiviral Ribavirin on MS medium to the growth and development of meristematic tissue (E1), shoot tip (E2), (2), antiviral Ribavirin consentration (0- 5- 10 mg/l) with MS supplement GA3 0.15 mg/l , kinetin 0.1 mg/l , CaP 2 mg/l , Myo inositol 100 mg/l , coconut water 100 ml/l ,sucrose 30 g/l , agar 6.5 g/l , pH 5.7 (3) The varieties were Granola, Median and Atlantic .The results of experiment (1) percentage growth and development meristematic on 4 WAP 50 - 80%, contamination 20 - 50%. (2) percentage of normal growth 40 - 50 , average number of shoot 0.25 - 0.95, the number of nodes 0.35 - 2.50, roots 0.20 - 0.93. (3) visual observation of antiviral Ribavirin on MS media visually influences growth and explant shoot tip growth better than meristem of the three variety .

Keywords: Potato ( Solanum tuberosum L)- Antiviral Ribavirin - Meristematic tissue

Topic: Plant Breeding and Biotechnology

Plain Format | Corresponding Author (Asih Kartasih Karjadi)

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