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181	Integrated Pest and Disease Management	ABS-49
Detection and Identification of Aphelenchoides fragariae nematodes on Shallot Bulbs in Bogor, West Java, Brebes Central Java and Nganjuk, East Java Devina Cinantya Anindita (a*), Supramana (b), Giyanto (b) a) Graduate School of Department of Plant Protection, Faculty of Agriculture, IPB University, Bogor. Indonesia *devina.anindita16[at]gmail.com b) Department of Plant Protection, IPB University, Bogor. Indonesia Abstract Shallot is one of the important horticultural commodities in Indonesia. Aphelenchoides fragariae is the major parasitic nematode of shallots and listed as quarantine pest with limited distribution. The nematode was detected on shallot bulbs at several traditional markets in Bogor but has not been reported present on shallot plantation. This research aims to detect and identify A. fragariae on shallot bulbs. Shallot bulbs were sampled from Bogor, West Java, Brebes, Central Java, and Nganjuk, East Java. Nematodes were extracted from shallots bulb by cold water immersion method at 20 oC. Morphological identification was carried out by following the standard taxonomic for the Aphelenchoides genus (Aphelenchoididae). Morphometric indications were based on the de Man^s formula. The result showed that A. fragariae were found on shallot bulbs from Bogor, Brebes and Nganjuk. The female of A. fragariae has a slender body and off-set lip characteristic distinguishes it from other nematodes. The stylet was invisible, but the metacorpus (median bulb) was visible and full rounded, tail end with single mucro. The morphometric A. fragariae are n=40, L = 312.2 - 550.5, a = 20.0 - 33.9, b = 7.0 - 12.0, c = 3.9 - 15.2, V = 34.4 - 84.4, T = 28.8 - 121.3, stylet 6.8 -12.0. Keywords: foliar nematode, morphology, morfometric, parasitic nematode Share Link | Plain Format | Corresponding Author (Devina Cinantya Anindita)

182	Integrated Pest and Disease Management	ABS-52
The Farmer Behavior Using Perticide in Maize (Zea mays L.) Sri Nur Aminah (a*), Tamrin Abdullah (a), Fatahuddin (a), Yuliani (b), Dhini Wirasti (a) a) Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jalan Perintis Kemerdekaan KM. 10 Tamalanrea Makassar 90245 INDONESIA b) Agrotechnology, Faculty of Applied Sciences, Suryakencana University Jl. Pasir Gede Raya No. 13, Cianjur Regency 43216, West Java INDONESIA *email: srifirnas[at]gmail.com Abstract Pesticides is the toxic compounds that contain synthetic chemical and most popularly used by farmers in crop management. The harmful of synthetic material contained in pesticides are capable of rapidly killing target organisms. However, continuous use of pesticides killed more natural enemies as the important biological agent to control insect pests. The pesticide residues product from chemical compound as the main source of environmental pollution. The purpose of the research is to determine the behavior of farmers used pesticides in the maize plantation. The research was conducted in Tenri Pakkua village, Bone Regency, South Sulawesi Indonesia, from January to March 2020. The research activities used a survey method in the form of interviews used a questionnaire to 35 farmers as a respondent. The results was showed that 97.15% of respondent controlled weed used herbicides, then 2.85% of respondents chose to control manually for the same weed. In addition, 62.86% of the respondent used herbicides for more than four years, about 28.57% used herbicide with average more than two years, respectively. The most insecticides used by respondent to control insect pests in maize from the active ingredients such as: Permethrin (42.87%), Fipronil (14.29%), Lambda-cyhalothrin (11.44%) and Metomil (8, 58%), respectively. The active ingredients roles as respiratory poison to insect. The result was showed that farmers prefer used pesticides in the form of synthetic herbicides and insecticides because the results are obvious, they quickly kill the target organism, the application does not require special skills and is easy to obtain (commercially available). Keywords: herbicide, insecticide, permetrine, maize, respondent Share Link | Plain Format | Corresponding Author (Sri Nur Aminah)

183	Integrated Pest and Disease Management	ABS-54
The Use of Endophyte Fungal Isolates in controlling Fusarium oxysporum, The Causal Agent of Wilt Disease on Chili (Capsicum annuum) Erma Dewi*, Ade Rosmana, Tutik Kuswinanti Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jl. Perintis Kemerdekaan KM. 10 Tamalanrea Makassar 90245 Indonesia Abstract Fusarium oxysporum as the causal agent of wilt disease infects systemically on the chili plant (Capsicum annuum) and cause a significant loss on its production. To control this fungus, we isolated and identified beneficial fungi from stem, leaf, and fruit tissues. Then, the work tested fungi capability to inhibit the pathogen in vitro and to enter of plant tissues. The results of the work indicated that seven fungus isolates consisted of Trichoderma 1, Trichoderma 2, Aspergillus, Fusarium 1, Fusarium 2, Lasiodiplodia 1, and Lasiodiplodia 2 were identified. Trichoderma and Lasiodiplodia were found from leaf and fruit tissues, whereas Aspergillus and Fusarium were isolated from leaf and stem tissues. The inhibition of F. oxysporium by these seven fungal isolates in vitro nine days post dual culture was 53.9%, 62.5%, 38.9%, 38,3%, 26.9%, 29,4%, and 34.7%, respectively. Endophyte study with Trichoderma morphospecies 2 showed that the isolate could colonize 84% of the root, 60% of the stem, and 75% of leaf tissues three weeks after inoculation through roots. Therefore, the research results demonstrate the presence of endophytic fungus derived from the chili plant that is potential to control wilt disease in vivo. Keywords: chili, endophytic fungus, Fusarium oxysporum, Trichoderma, wilt disease. Share Link | Plain Format | Corresponding Author (erma dewi)

184	Integrated Pest and Disease Management	ABS-65
What a significant difference between symptoms caused by Ceratobasidium theobromae and Lasiodiplodia theobromae? Muhammad Junaid Universitas Hasanuddin Abstract Vascular streak dieback disease symptom is clearly defined as symptoms were chlorosis and later necrosis of leaves, mostly those second or third from the stem tips. Abscission of these leaves occurred within 2 or 3 days of the first appearance of symptoms. Swelling of lenticels was distinct on the bark surface. This symptom is undoubtedly caused by Ceratobasidium theobromae. However, most recent articles also explained that the symptom was also associated with another fungal pathogen, Lasiodiplodia theobromae, which has involved and contributed to give a sign of such a VSD disease lesion. This paper will explain a very fundamental difference of environmental-based symptom on cocoa leaves between caused by C. theobromae and L. theobromae. This paper also will review the ways to analyses environmental symptom, detect molecularly method and test pathogenicity previously conducted before we conclude that specific symptomatic lesion of VSD disease is only caused by a single pathogen as C. theobromae. Keywords: Symptom analysis of VSD disease on cocoa Share Link | Plain Format | Corresponding Author (Muhammad Junaid)

185	Integrated Pest and Disease Management	ABS-66
Microbes occupying cocoa branches and their consequences Muhammad Junaid1, Mutiah1, Ayu Parawangsah2 1) Universitas Hasanuddin 2) Universitas Muslim Indonesia Abstract Cocoa is a typical pantropical tree as it only grows at the environments-based equator line which is originated from Latin Americas. Since it had been cultivated in Indonesia in 18 Century, number of indigenous living microbes were reportedly identified. However, the interaction among microbial stem tissues is unclear. The study aims to understand number of microbes and its association with cocoa production system. Keywords: Microbial association with cocoa branches Share Link | Plain Format | Corresponding Author (Muhammad Junaid)

186	Integrated Pest and Disease Management	ABS-68
The Effectiveness of Rhizosphere Bacteria in Inducing the Resistance of Maize to Downy Mildew Peronosclerospora philipinensis NURASIAH DJAENUDDIN, MUHAMMAD AZRAI, TUTIK KUSWINANTI, BAHARUDDIN PATANDJENGI 1. Program of Magister, Department of Plant Pests and Diseases, Faculty of Agriculture, University of Hasanuddin 2. Indonesian Cereals Research Institute Abstract Downy mildew caused by Peronosclerospora philipinensis is a major disease of maize. The disease is currently managed by seed treatment with metalaxyl fungicides, but the development of pathogenic fungi resistance towards synthetic fungicides is a great problem affecting significantly the future of chemical control by fungicides. The use of microorganisms for induction of systemic resistance against pathogens on maize is well known. Therefore, this study aims to determine the efficacy of bacterial isolates from the rhizosphere in influencing the resistance of maize to downy mildew by measuring accumulated concentration of salicylic acid. Treatments were based on a fully randomized 2-factor design with 3 levels of- factor I (bacteria) 3 levels (b0 = no bacteria, b1 = Bacillus paramycoides Ga-3, b2 = B. cereus Si-4) and factor II (salicylic acid) 2 levels (as0 = 0 g/L and as1 = 2 g/L). The research was carried out at the screenhouse and plant pathology laboratory, Indonesian Cereal Research Institute (ICERI), from March to July 2020. Each treatment combination was tested on three varieties of maize, namely Anoman, Bima20, and Bima3, hence there were 18 treatment combinations. Furthermore, the results showed that the use of rhizosphere bacteria and salicylic acid has the potential to control downy mildew in maize. In the case of the Bima20 variety, the combined treatment of B. paramycoides Ga3 + salicylic acid had the lowest incidence of disease. Meanwhile, treatment of B. cereus Si4 + salicylic acid in Bima3 variety had the lowest incidence of disease, namely 13.9% and 26.1%, respectively. Considering the results, the enrichment of salicylic acid content was highest at 9.6 ppm on maize which were applied with the rhizosphere bacterium B. paramycoides Ga3 + salicylic acid. Keywords: chlorophyll content, leaf stomata, variety Share Link | Plain Format | Corresponding Author (Nurasiah Djaenuddin)

187	Integrated Pest and Disease Management	ABS-69
The Mortality of Green Planthoppers Nephotettix virescens (Homoptera: Cicadellidae) by Metarhizium anisopliae Metchn and Beauveria bassiana (Bals.) Vuill Tamrin Abdullah (a*), Sri Nur Aminah (a), Fatahuddin (a), Nurul Wirid Annisaa (b) a) Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jl. Perintis Kemerdekaan KM. 10 Tamalanrea Makassar 90245 INDONESIA b) Magister Student of Phytopathology, Post Graduate Program, Bogor Agricultural University, Jalan Raya Dramaga Babakan, Kecamatan Dramaga, Bogor 16680, West Java, INDONESIA Abstract The green planthopper Nephotettix virescens Distant (Homoptera: Cicadellidae) is one of the important pests of rice plants in South and Southeast Asia. N. virescens caused damages of rice plants by sucking rice fresh fluid and vector virus of tungro disease. The purpose of research is to determine the effectiveness of two types entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana in suppressing the population level of N. virescens on rice plants. The research activities was held at the Laboratory of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar from September 2018 to June 2019. The research used a Factorial Completely Randomized Design with eight treatment and five replications. Each treatment used 10 individual nymphs of N. virescens. The research started from the collection and mass rearing of N. virescens, prepared the rice plants, cultured of M. anisopliae and B. bassiana, application of the entomopathogenic fungi M. anisopliae and B. bassiana. The application of M. anisopliae and B. bassiana on the fifth day was showed the average mortality of N. virescens by spraying (89.6%), watering (89.6%) and dipping (89.6%) not significantly. However, the mortality was higher compared the control (0.50%). Watering treatment used the suspension of B. bassiana showed a very high average mortality compared dipping used M. anisopliae. The application of M. anisopliae in the spraying and watering treatments showed the average mortality not significantly different compared to the spraying treatment used entomopathogenic B. bassiana. Keywords: Metarhizium anisopliae, Beauveria bassiana, Nephotettix virescens, rice Share Link | Plain Format | Corresponding Author (Tamrin Abdullah)

188	Integrated Pest and Disease Management	ABS-72
Insect Pest in Rice Field with Legumes Plot Applied by Beauveria bassiana Vuill Sri Nur Aminah (a*), Tamrin Abdullah (a), Rosi Widarawati (b), Nurul Wirid Annisaa (c), Fitri (a) a) Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jalan Perintis Kemerdekaan KM. 10 Tamalanrea Makassar 90245 b) Agrotechnology Program, Faculty of Agriculture, Jenderal Soedirman University, Jalan Dr. Soeparno No. 61, Karangwangkal Purwokerto 53123, Central Java, Indonesia c) Magister Student of Phytopathology, Post Graduate Program Bogor Agricultural University, Jalan Raya Dramaga, Babakan Kecamatan Dramaga Bogor 16680, West Java, Indonesia *e-mail: srifirnas[at]gmail.com Abstract The research objective is: to study diversity of insect pests in rice field used plot grow of legumes and effect spraying of entomopathogenic B. bassiana. Observation rice pests and legumes plant was held in Mappadaelo village, Wajo Regency, South Sulawesi. Identification of insect pests held at the Pest Laboratory, Faculty of Agriculture, Hasanuddin University Makassar, from August to October 2019. Experiments used Mekongga as the rice variety grow in four different fields, distance 200 - 300 m. The plot with legumes (mung bean, cowpea and long bean), planting a week after rice. The methods of cultivating rice and legumes plants based on the habit of farmers. The study used a Randomized Block Design with eight treatments and four replications. The observation insect pests in both plant started from 14 days after planting with 10 samples. Insect collection used D-Vac and put in bottle containing alcohol. The suction of D-Vac has interval seven days. The result showed: the highest diversity of insect pests without B. bassiana spraying found in rice and mung beans (P1) of -0.71. The lowest result was showed P3 (rice and long bean) about -0.65. The diversity of insect pests in rice was sprayed B. bassiana about -2.28. The highest diversity index by spraying B. bassiana found at P7 (rice and long beans) of -0.75. The lowest insect diversity index was P6 (rice and cowpeas) of -0.45. The conclusion: spraying of the B. bassiana showed effect on the diversity index of insect pests in rice field. Keywords: B. bassiana, rice, mung bean, cowpea, long bean Share Link | Plain Format | Corresponding Author (Sri Nur Aminah)

189	Integrated Pest and Disease Management	ABS-74
The Effectivity Trap Based Plastic Waste in Monitoring of Insect Pests Sri Nur Aminah (a*), Mansur (b), Salim (c), Suyono (d) a) Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jalan Perintis Kemerdekaan KM. 10 Tamalanrea, Makassar 90245, INDONESIA b) Centre Research of Tungro Diseases, Jalan Buli No. 101 Lanrang, Sidrap 91651, South Sulawesi, INDONESIA c) Assessment Institute for Agriculture Technology (AIAT) South Sulawesi Jalan Perintis Kemerdekaan KM. 17.5 Makassar 90245 INDONESIA d) Agrotechnology, Faculty of Agriculture and Forestry, West Sulawesi University Jalan Prof. Dr. Baharuddin Lopa, SH, Talumung, Majene 91412, South Sulawesi, INDONESIA *email: srifirnas[at]gmail.com Abstract The plastic waste around us such as: plastic bottle and glass from drinking water very useful modified as tools for monitoring insect pests in agricultural ecosystem. The presence of insect pest in farmer plantation need more attention such as monitoring for exactly management effort. The purpose of research is to re-use plastic waste became yellow trap and pitfall trap for monitoring of insect pests attack the popular vegetable. The advantages of research as new information for community used plastic waste as tools of monitoring insects. The observation was held in kangkong and spinach plantation used pit-fall trap and yellow trap for monitoring presence arthropods in the planting season. The results was showed: the effectivity of pit-fall trap from plastic waste used for arthropods monitoring especially predator in kangkong field about 45% and insect pests 37.5%. As the comparing data, the effectivity of pit-fall trapped predator in spinach plantation 99.2%, lowest number in insect pests and decomposer about 0.4%, respectively. The result of yellow trap made from plastic bottle in kangkong field trapped insect pests about 43.7% and decomposer 41.1%. The trapped of insect pests used yellow trap in spinach plantation 63.4% and predator 26.6%. The coclusion of research: plastic waste more benefit as yellow and pit-fall trap for monitoring insects. The effectivity of tools depend on arthropods composition as important part of ecosystem. The reduce of pesticide application and management of flowering weeds improving number of beneficial insect as biological control agent in agroecosystem. Keywords: pit-fall trap, yellow trap, monitoring, plastic waste, insect Share Link | Plain Format | Corresponding Author (Sri Nur Aminah)

190	Integrated Pest and Disease Management	ABS-78
Dynamics of Tungro disease and its vector population on intercropping of rice varieties Riski Apriyani*, Ade Rosmana, Baharuddin Patandjengi Plant Pests and Diseases Department, Faculty of Agriculture, Hasanuddin University Jl. Perintis Kemerdekaan KM.10 Tamalanrea Makassar 90245 Indonesia *corresponce author: riskiapriyani15[at]gmail.com Abstract Tungro is one of the major diseases that can reduce theyield potential of rice. The disease is caused by rice tungro baciliform virus (RTBV) and rice tungro spherical virus (RTSV), both of which can only be transmitted by green leafhoppers, Nephotettix virescens in a semi-persistent manner. The research aimed to control both the disease and the vector by using intercropping of rice varieties. The optimum of disease incidence in 11 weeks observation on the variety of Ciherang, Inpari 32, Inpari 36, and IR 64 planted in intercropping was 7.8%, 7.3%, 3,8%, and 6.3%. Whereas, the incidence on these respective variety planted in monoculture was 9.8%, 8,8%, 4.3%, and 8.5%. Then, the optimum of N. virescens population per clump on rice variety with interplanting in the same time of observation was 4.8, 2.3, 1.5, and 4, and on variety with monoculture was 6.5, 4.5, 3.3, and 4,5, respectively. Moreover, the population of insect predators tended to be higher on variety with interplanting than on rice variety with monoculture. These data indicated that rice planted with variety interplanting was related to the reduction of tungro incidence and its vector and also the increase of insect predators. Therefore, the interplanting of variety could be used as one of control method against rice pests and diseases. Keywords: Incidence, interplanting, Nephotettix virescens, population, rice variety, tungro. Share Link | Plain Format | Corresponding Author (Riski Apriyani)

191	Integrated Pest and Disease Management	ABS-80
Resistance Validation of Several Chili Pepper Lines to Anthracnose Colletrothicum acutatum Isolate Sukabumi Neni Gunaeni1, Eli Korlina, Redy Gaswanto Indonesian Vegetables Resarch Institute Jln. TangkubanPerahu No. 517 Lembang-Bandung Barat (40391) *Correspondence E- mail: nenigunaeni63[at]gmail.com Abstract Anthracnose is the most dominant disease that infects to chili pepper and it can reduce yield. One technique to control the disease is using resistant variety. The objective research was to ensure resistance level of the screened chili pepper lines to C. acutatum as the most virulent pathogen. The research was conducted at Indonesian Vegetables Research Institute from June to December 2019. C. acutatum isolate Sukabumi was used as an inoculum. This isolate had a DNA band 490 bp according to PCR identification. The used screening method was artificial in the laboratory by inoculating 5 ul of conidial suspension with a density of 5.0 x 105 per one injection per fruit. PR protein analysis was performed on infected fruit and healthy. The selected lines results obtained that 2A (R-4), 4A (R-15), 4B (R-9), 6 (R-12), 7 (-13 ), 8 (R-14) were resistant to anthracnose C. acutatum isolate Sukabumi. The protein band pattern profile by PR-protein analysis showed that a gene expression with molecular weight around 37 kDa. Keywords: Capsicum annuum, Colletrotrichum acutatum, Chili pepper lines, Isolate Sukabumi Share Link | Plain Format | Corresponding Author (Neni Gunaeni)

192	Integrated Pest and Disease Management	ABS-88
Assessing disease severity and incidence of rice blast under natural infections in different rates of Nitrogen and planting system Cipto Nugroho(1,*), Didik Raharjo (1), Muhammad Alwi Mustaha (1), and Muhammad Asaad (1,2) 1) Assessment Institute of Agricultural Technology Southeast Sulawesi, Indonesian Agency of Agricultural Research and Development, Jl. M. Yamin No. 89, Puwatu, Kendari, Sulawesi Tenggara, Indonesia 2) Assessment Institute of Agricultural Technology South Sulawesi, Indonesian Agency of Agricultural Research and Development, Jl. Perintis Kemerdekaan Km. 17.5, Sudiang, Makassar, Sulawesi Selatan, Indonesia * corresponding author: ciptonugroho[at]gmail.com Abstract The study aimed to determine the effect of nitrogen rates and planting systems on incidence and severity of rice blast under naturally infected rice plants and its relationship to rice yield performance. Recommended nitrogen rate for both direct seeding was 93.2kg/ha. Treatments consisted of 75% recommended-N rate, 100% recommended-N rate, 150% recommended-N, and existing farmer^s practice. Treatments were applied in a three-way splitted-N application at 14, 30, and 45 days after planting, respectively. The results indicate that rates of N fertilization affected disease incidence of the rice blast where low-N application was generally lower than recommended-N rate, high-N rate, and farmer^s practice, both on jajar legowo 2:1 and broadcasted direct seeding. This study revealed that recommended-N application, although has higher disease incidence than low-N application, could tolerate yield loss due to rice blast by producing more productive tillers, number of grains per panicle, and reducing number of unfilled grains. This study generally showed that jajar legowo 2:1 direct seeding gave lower disease incidence and severity to the rice blast than broadcasted direct seeding. The study suggested recommended-N rate, based on soil test kit of IAARD, and jajar legowo 2:1 direct seeding could be incorporated to the rice blast management strategies. Keywords: rice blast, nitrogen rates, planting system, disease incidence Share Link | Plain Format | Corresponding Author (CIPTO NUGROHO)

193	Integrated Pest and Disease Management	ABS-94
Farmers^ Knowledge on Pesticide Management Practices: A Case Study of Shallot Growers in Palu, Indonesia Kasman Jaya1, Ratnawati Ratnawati1, Asman Asman2*, Arfan Arfan1 1Faculty of Agriculture, Alkhairaat University, Palu, Central Sulawesi, Indonesia 2 Department of Plant Pests and Diseases, Hasanuddin University, South Sulawesi, Indonesia Abstract Pesticides are one of the most common methods of pests and diseases control. Knowledge of pesticides management is significant for effective application. The purpose of study was to determine knowledge of farmers about pesticides management practices on shallot crops in Palu, Central Sulawesi, Indonesia. The study was conducted from October 2017 to February 2018. This quantitative and survey research was used respondents of 30 farmers in two locations: Duyu and Bayaoge in Tatanga district, Palu, Central Sulawesi, Indonesia. Commonly, the respondents working on shallot field. The data quantification using the ordinal scale, then univariate analyzed for frequency distribution and bivariate analysis to determine the correlation of two variables. The results was showed there no significant effect between farmers knowledge about pesticides and the actions of farmers in the use of pesticides. Increasing knowledge about pesticides cannot be expected to change wise actions in using pesticides in pest control. Keywords: Pesticide management, Farmers^ Knowledge, shallot, Palu Share Link | Plain Format | Corresponding Author (Asman Asman)

194	Integrated Pest and Disease Management	ABS-100
Host Range OF Exallomochlus hispidus (Morrison) (Hemiptera: Pseudococcidae) in West Java Murni Indarwatmi(a*), Indah Arastuti Nasution(a), Endang Sri Ratna(b), Dewi Sartiami(b), Arinana(c), Sulaeha Sulaeha(d), Yani Maharani(e) (a) Centre for Isotope and Radiation Application, National Nuclear Energy Agency, Jakarta 12440 Indonesia *murninda[at]gmail.com (b) Department of Plant Protection, Faculty of Agriculture, IPB University, Bogor 16880, West Java, Indonesia (c) Forest Product Departemen, Faculty of Forestry, IPB University, Bogor 16880, West Java, Indonesia (d) Faculty of Agriculture, Universitas Hasanuddin, Jl. Perintis Kemerdekaan KM 10, Tamalanrea, Makassar, South Sulawesi, Indonesia (e) Faculty of Agriculture, Universitas Padjajaran, Jl. Raya Bandung Sumedang, Km 21, Jatinangor, Sumedang District, West Java, Indonesia Abstract Exallomochlus hispidus (Morrison) (Hemiptera: Pseudococcidae) is a polyphagous mealybug with a wide host range. Many of them are fruits that have high economic value and potential export comodities. In addition, E. hispidus is a quarantine pest so that its presence can be an export barrier. The purposive sampling method were used to take plant samples. Host plants were sampled in 4 districts in Bogor, Sukabumi, Purwakarta, and Garut, West Java. Results showed that E. hispidus was found on 29 specieses of 17 families. This species is most commonly found on cocoa Theobroma cacao, mangosteen Garcinia mangostana, soursop Annona muricata, sugar apple Annona squamos, sapodila Manilkara zapota, and duku Lansium domesticum. The results of this study are expected to provide information on the host range of E. hispidus so that it can be prevented from spreading and proper control is taken. Keywords: fruit export commodities, host plant, mealybug, polyphagous, quarantine pest Share Link | Plain Format | Corresponding Author (Murni Indarwatmi)

195	Integrated Pest and Disease Management	ABS-106
The Potency of Bintaro Leaf Infusion for Controlling Meloidogyne incognita and the Growth of Okra Zahra Rahmadiyanti (a*), Abdul Munif (a) Faculty of Agriculture, Bogor Agricultural University Jl. Raya Dramaga, Bogor, Jawa Barat 16680 zahra_rd[at]apps.ipb.ac.id Abstract Meloidogyne incognita is one of the important plant parasitic nematodes which causes root-knot in okra (Abelmoschus esculentus). The use of bintaro (Cerbera manghas) leaf infusion is expected to be an alternative for controlling root-knot nematodes. Bintaro contains bioactive compounds that can be used as a bionematicide. Bintaro leaves were blended, soaked in rice bran and water solution then incubated by aerated and non-aerated condition for three weeks. The infusions were filtered and stored in room temperature. The concentrations of infusion were used in this study are 25%, 50%, and 75%. The result showed that under in vitro test bintaro leaf infusion caused percent mortality of Meloidogyne incognita from 25% up to 100% in 24 hours after treatment. Application of bintaro leaf infusion has no phytotoxic effect on okra. Treatment with BA (aerated bintaro leaf infusion on rice bran solution) 75% and BN (non aerated bintaro leaf infusion on rice bran solution) 75% were able to increase wet weight and dry weight of okra. This research indicates that infusion of bintaro leaf have potential to be developed as a bionematicide. Keywords: bioactive compound, bintaro, bionematicide, Meloidogyne incognita. Share Link | Plain Format | Corresponding Author (Zahra Rahmadiyanti)

196	Integrated Pest and Disease Management	ABS-108
Optimization of Automatic Repellent Tool to Repel Pests of Rice-Eating Birds in Takalar, South Sulawesi Muhammad Yusril Hardiansyah (a*), Nurhidayat (b), Abdul Mollah Jaya (a), Muhammad Arif Fikri Al Ridho (a) a) Department of Agronomy, Agrotechnology Study Program, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia b) Department of Soil Science, Agrotechnology Study Program, Faculty of Agriculture, Hasanuddin University, Makassar, Indonesia Abstract Bird pests are one of the main enemies for farmers which can reduce crop production. Increasing bird populations lead to reduced yields. This is a major problem that causes the stability of rice production in Takalar, South Sulawesi to shake every year. So that one method that can be used to repel bird pests is to use an automatic bird pest repellent tool. This study aims to determine the impact of using automatic repellent to reduce the attack of rice-eating birds in Takalar. This research has an effect on a better rice cultivation process, especially in reducing the level of bird pest attacks. This research was conducted in Tonasa Village, Takalar Regency, South Sulawesi. The research implementation method includes designing a pest repellent method using an automatic air freshener machine as well as the manufacture and testing of jengkol extract vegetable pesticide liquid as a spraying agent that emits a scent that bird pests don^t like. Observation parameters used the comparison method of initial observations and final observations that were observed in the morning, afternoon and evening. The evaluation results obtained on average show quite a lot of attacks in a day, especially in the morning. After the use of the automatic repellent method, the attacks of bird groups tended to decrease where in the morning the ratio of 22 - 32 decreased to 6 - 14 attacks, while the afternoon ratio of 7 - 14 decreased to 3 - 7 attacks and the evening ratio of 20 - 29 decreased to 8 - 12 group attack of birds. So that the use of this automatic repellent has a significant and effective impact in reducing bird pest attacks. Keywords: Automatic Repellent Tool, Bird Pests, Rice, Takalar Share Link | Plain Format | Corresponding Author (Muhammad Yusril Hardiansyah)

197	Integrated Pest and Disease Management	ABS-117
The Effectiveness of Filtrate Culture with Fusarium Fungi .spp Against Meloidogyne spp. Nematodes In-Vitro on Tomato Plants Muhammad Nuzul Ramadhan (a*) , Nur amin (b), and Andi Nasruddin (b) a) Magister Program, Department of Plant Pest and Disease, Faculty of Agriculture Hasanuddin university, Makassar , South Sulawesi 90245, Indonesia. b) Department Of Plant Pest and Diseases, Faculty of Agriculture Hasanuddin university, South Sulawesi 90245, Indonesia. Abstract Parasitic nematodes are one of the important plant pests that attack various types of cultivated plants. In Indonesia, 26 species of parasitic nematodes that attack food crops, horticulture and plantations have been identified. One such nematode, Meloidogyne, is the most destructive parasitic nematode. Utilization of other microorganisms as natural enemies of nematodes from the fungal group can be used as biological agents. This research was conducted at the Laboratory of Disease, Department of Pests and Plant Diseases, Faculty of Agriculture, Hasanuddin University Makassar. This study aims to determine the ability of Fusarium Fusarium isolates. . The results showed that the application of Fusarium isolate (Fusarium .spp) and the given liquid media was able to cause juvenile mortality of root knoot nematodes (Meloidogyne sp.). And the time required by Fusarium fungi .spp and liquid media to cause nematode mortality was 24 hours, but the highest mortality occurred at 9 hours after application, namely 7.74 at the BNJ test level of 0.05%. Keywords: Meloidogyne sp, Fusarium .spp, Filtrat culture , Mortalitas Share Link | Plain Format | Corresponding Author (Muhammad Nuzul Ramadhan)

198	Integrated Pest and Disease Management	ABS-120
Antagonistic Activity of Volatile Organic Compounds of Endophytic Bacteria from Sword Brake Fern (Pteris ensiformis) Against Soil Borne Fungal Pathogens Abdul Munif (a), Prayogo Probo Asmoro (b*) a) Department of Plant Protection, Faculty of Agiculture, IPB University b) Graduate School Department of Plant Protection, Faculty of Agiculture, IPB University *prayogoasmoro[at]apps.ipb.ac.id Abstract The use of endophytic bacteria in the control of plant diseases, especially soil pathogenic fungi such as showed good results. Endophytic bacteria have various mechanisms to control plant pathogen, but the mechanism of endophytic bacteria in producing volatile organic compounds (VOCs) has not been widely studied. The objective of this research was to evaluated the antagonistic activity of VOCs produced by endophytic bacteria from the nail sword (Pteris ensiformis) aginst three soil borne pathogen fungus, R. solani, S. rolfsii and Fusarium sp. Endophytic bacterial isolates used were APE15, APE22, APE33, and APE35. The study was conducted by cupping method to determine the inhibition of endophytic bacteria VOCs against three soil borne fungal pathogens. GC-MS analysis was conducted to determine the content of volatile compounds produced by endophytic bacteria. The results showed that four endophytic bacterial isolates can inhibited R. solani growth (92.11%-96.05%), S. rolfsii (87.28%-93.10%), and Fusarium sp. (27.73%-57.55%). The GC-MS analysis showed that there were several antifungal compounds detected i.e. octanal, cytronellyl acetate, silane, 9-octacenamide, n-dimetylpalmitamide, and isobutyryl chloride. Octanal was presumed as the most influential compound that inhibited the three soil borne fungal pathogens because it was a dominant compound was detected. Keywords: Biological control, GC-MS, octanal, Rhizoctonia solani Share Link | Plain Format | Corresponding Author (Prayogo Probo Asmoro)

199	Integrated Pest and Disease Management	ABS-125
Implication of types of feeds combined goat manure for preference black soldier fly (bsf) : Hermetia illucens L. Indri, S Sjam, A Gassa, VS Dewi, PA Palayukkan Universitas Hasanuddin Abstract . Hermetia illucens L. (Diptera: Stratiomyidae) called black soldier fly(BSF) has an important role in the degradation of organic waste or bioconversion activity. The current study compared some types of feeds to preference BSF to eggs laid and the implication of of the number of larvae and the size of the pupae produced , the percentage of larvae that became adults. Type of feeds is rice bran, mixture of fruits waste, agricultural waste, vegetable waste and household waste. The types of feeds mixed with goat manure.The study reports that Black Soldier Fly (BSF) produced the highest number of egg colonies in household waste (6 colonies) then vegetable waste (5 colonies), bran (4.3 colonies), fruit waste (3.3 colonies) and the lowest on agricultural waste (0.3 colonies). The highest number of larvae was in bran (4783), then household waste (1942), vegetable waste (1511), fruits waste (983) and the lowest in agricultural waste (92 ). The longest pupa size is in fruits waste (2 cm), household waste (1.96 cm), vegetable waste and the lowest is in agricultural waste(0.48 cm). The highest number of imago was in bran (1606 heads), vegetables waste (211 heads), fruits waste (137 heads), restaurant waste (93 heads) and the lowest was in agricultural waste (10 heads). Keywords: Hermetia illucens Share Link | Plain Format | Corresponding Author (Hamdayanty Hamdayanty)

200	Integrated Pest and Disease Management	ABS-126
Growth Inhibition of Spodoptera frugiperda using Combination Calotropis gigantea L. and Crescentia cujete L. AS Aprialty, S Sjam, VS Dewi, and YE Agustina Universitas Hasanuddin Abstract Spodoptera frugiperda is an invasive insect and has spread in various countries, including Indonesia, which attacks almost all maize crops. This study purpose to determine the development inhibition of the combination C. gigantea extract and C. cujete extract. The research was implemented at the Pesticides and Natural Materials Laboratory, Faculty of Agriculture, Hasanuddin University. Observations doing at 2 stages, that is testing various concentration of C. gigantea and C. cujete extracts on mortality and development inhibition larva S. frugiperda, and then for concentration 7,5% that caused the high mortality, it was further tested by combining between the extract of C. gigantea and C. cujete. The results showed that C. gigantea extract caused high mortality at a concentration of 7.5% and 10% with LC50 at a concentration 7.36% and LC90 at a concentration 9.08%. C. cujete extract caused high mortality at a concentration of 5% up to 10% with LC50 at a concentration 5.96% and LC90 at a concentration 8.06%. The percentage development inhibition of S. frugiperda larvae each reached 100% using extract C. gigantea concentration 10% and extract C. cujete concentration 10%, while the percentage of inhibition using a combination extract reached 100% at a concentration 7.5%. The high mortality and percentage development inhibition of S. frugiperda larvae was found in the use of C. cujete extract compared with C. gigantea, and if two extracts were combined, it caused the highest mortality and development inhibition compared without the combination. Keywords: Spodoptera frugiperda, C. gigantea, C. cujete Share Link | Plain Format | Corresponding Author (Hamdayanty Hamdayanty)

201	Integrated Pest and Disease Management	ABS-127
Application of the combination of Calatropis gigantea L. and Crescentia cujete L. against Scirpophaga innotata and Leptotocorisa acuta Thunb. and predator in paddy plants PA Palayukkan1, S Sjam1, M Melina1, Sulaeha1, A Rosmana1, VS Dewi1 and Budirman2 Universitas Hasanuddin Abstract Abstract. C. gigantea L. and C. cujete L. are plants that contain secondary metabolites that can act as insecticides. The aim of this study was to evaluate the implications of C. gigantea and C. cujete to reduce the population and intensity of attack by stem borer (S. innotata) and stink bug (L. acuta) which are the main pests of paddy plants. This study consisted of 4 treatments consisting of a mixture of C. gigantea and C. cujete extracts, C. gigantea extracts, C. cujete extracts, and control with 5 replications. Extract made by fermentation and the extract was applied at the age of 1 month after planting with a concentration of 5% for 8 times with a frequency of 1 time a week. Observation parameters were population and attack intensity of S. innotata and L. acuta as well as natural enemy populations. The results obtained were that the treatment of all extracts could reduce the population and intensity of attacks compared to the control. Treatment of the mixture of C. gigantea and C. cujete extracts can reduce the population and the intensity of attacks is higher than without the mixture.Predator is not affected by extract applications. Keywords: C. gigantea L. ,C. cujete, extract, S. innotata and Leptocorisa acuta Share Link | Plain Format | Corresponding Author (Hamdayanty Hamdayanty)

202	Integrated Pest and Disease Management	ABS-128
Molecular Detection of Tungro Virus in Mekongga and Inpari Rice Varieties in West Papua H Hamdayanty, FT Ladja, Mansur Universitas Hasanuddin Loka Penelitian Penyakit Tungro Abstract Tungro disease is one of the important diseases in rice plants. One of the efforts to reduce the spread of the tungro virus is to ensure the presence of the virus in the field. This is the first step in efforts to prevent the spread and control of tungro disease, especially in West Papua. One detection technique that can be done is molecular detection through PCR techniques. Rice samples detected were Mekongga and Inpari varieties taken from rice plants in West Papua. Total DNA of RTBV, which is one of the viruses that cause tungro disease, was extracted and amplified using DAF primers (5-GGAATTCCGGCCCTCAAA AACCTAGAAG-3) and DAR (5-GGGGGTACCCCCCTC CGATTTCCCATGTATG-3). The PCR RTBV results showed that the positive samples were infected with Rice tungro bacilliform virus (RTBV). This is indicated by amplification of DNA measuring +- 1400 bp which is the target size of the DAF and DAR primers. The results of this study are preliminary information that can be used as a basis for tungro control and recommendations for future cropping. Keywords: Rice tungro bacilliform virus, PCR, Tungro Share Link | Plain Format | Corresponding Author (Hamdayanty Hamdayanty)

203	Integrated Pest and Disease Management	ABS-130
Stem Borer Attack on Growth and Yield of Rice (Oryza sativa L.) in Bali, Indonesia I.B.K. Suastika, I.B.G. Suryawan dan S.A.N. Aryawati Assessment Institute for Agricultural Technology of Bali Indonesia Abstract Research was conducted in subak Guama, Selanbawak Village, Marga District, Tabanan Regency from January up to April 2017 and subak Dlod Sema, Sading Village, Mengwi District, Badung Regency from Januari up to Mayi 2017. This study aims to understand the growth, productivity and resistance to stem borer pest of superior rice varieties cultivated in Bali Province, Indonesia to substitute Cigeulis and Ciherang. Research was arranged in Randomized Block Design with some superior rice varieties as treatment namely Inpari 30 and Inpari 40 in Tabanan Regency, and also, Inpari 16, Inpari 24, Inpari 28, Inpari 30, Towuti and Situ Bagendit in Badung Regency. Meanwhile, Cigeulis and Ciherang were used as comparison. Each treatments was replicated 5 times. Parameters observed consist of growth and yield components such as plant height, number of tillers, number of grains per panicle, 1000 grains weight and yield. In addition, resistance to stem borer was also observed by record plant damages caused by stem borer using scoring technique. Result showed that Inpari 30 and Inpari 40 were seem to resistant to stem borer with percentage of damage were 7.5% and 1.0%, respectiely. The damages were in the light category and had a chance to be developed in the subak Guama, Tabanan Regency as a substitute for the Ciherang, since the production of Inpari 30 was 6.86 t/ha harvested dry grain (HDG) and Inpari 40 was 6.93 t/ha compared to 6.44 t/ha of Ciherang. Inpari 30 also has an opportunity to be developed in the subak Dlod Sema, Badung Regency with percentage of damages was 10%, it be included light category. The production of Inpari 30 was 6.08 t/ha compared to Ciherang was 4.35 t/ha. Inpari 30 and Inpari 40 can be cosidered as candidate to substitute Ciherang based on their agronomy performances and productivity, they were greater than Ciherang. moreover Inpari 40 was more suitable to substitute Ciherang based on its agronomy performances, productivity and resistance. Keywords: rice, resistance, stem borer, Ciherang substitute, superior variety, productivity Share Link | Plain Format | Corresponding Author (Sagung Ayu Nyoman Aryawati)

204	Integrated Pest and Disease Management	ABS-132
Isolation and Antagonism of Chitinolytic Bacteria from Ipomea pes caprae against Lasiodiplodia pseudotheobromae Andi Khusnul Fatima Bahar (a*), Baharuddin Patandjengi(a), Andi Nasruddin (a) a) Faculty of Agriculture, Department of Pest and Disease, Makassar 90245, Indonesia Abstract Chitin is the main component of fungal cell walls. The growth of fungi can be inhibited by chitinolytic bacteria because they can produce chitinase enzymes which play a role in degrading cell walls. The purpose of this study was to test the activity of bacteria isolated from the roots of Ipomea pes caprae in inhibiting the growth of the fungus Lasiodiplodia pseudothebromae . The research method was carried out by isolating as much as 5 g of I. pes caprae roots using specific media and testing for bacterial antagonism using a dual culture method . The results showed that there were four isolate codes obtained, namely IPaR1, IPaR4, IPpA2, and IPpA3 and could inhibit the growth of Lasiodiplodia pseudtheobromae. Keywords: Chitinolytic Bacteria, Lasiodiplodia pseudotheobromae, chitinase enzyme. Share Link | Plain Format | Corresponding Author (Andi Khusnul Fatima Bahar)

205	Integrated Pest and Disease Management	ABS-157
Potential biocontrol of endophytic fungi against Lasiodiplodia pseudotheobromae, causal agent of cocoa dieback on cocoa seedling V Membalik1, A Asman1,2*, N Amin1, A K F Bahar1 1 Department of Plant Pests and Diseases, Hasanuddin University, South Sulawesi, Indonesia 2 Cocoa Research Group (CRG), Faculty of Agriculture, Hasanuddin University, South Sulawesi, Indonesia Abstract Endophytic fungi associated with cocoa is one of the effective agents to control plant pathogens on cocoa, including the fungus Lasiodiplodia pseudotheobromae. L. pseudotheobromae cause a significant disease on cocoa, cocoa dieback. The disease is considered an emerged disease on cocoa in Sulawesi, Indonesia. The research aimed to determine the effect of the endophytic fungi on L. pseudotheobromae on cocoa seedlings.. This study used a randomized block design consisting of 6 treatments and 3 replications. Five different fungi isolates were used that belongs to Aspergillus genera. The treatment is three isolates of endophytes fungi (isolate T2S2LT, T245LT, T645LT), Aspergillus isolates combination, and control (with and without pathogen). The results showed that the combination of isolates and T645LT isolate was the best treatments on suppressing necrotic symptoms (leaf spot) incidence and severity. Also, T645LT isolate performed well inhibit leaf blight symptoms incidence severity. Meanwhile, chlorotic symptom was reduced by isolates combination, followed by T2S2LT, T645LT, and T245LT. Endophytic fungi can potentially be an important option to control cocoa dieback disease. Keywords: Endophytic Fungi, Lasiodiplodia pseudotheobromae Share Link | Plain Format | Corresponding Author (Asman Asman)

206	Integrated Pest and Disease Management	ABS-158
The effect of several fungicides against Lasiodiplodia pseudotheobromae isolated from cacao through in vitro test Musdalifa1, Asman Asman1,2*, Ade Rosmana1,2 1 Department of Plant Pests and Diseases, Hasanuddin University, South Sulawesi, Indonesia 2 Cocoa Research Group (CRG), Faculty of Agriculture, Hasanuddin University, South Sulawesi, Indonesia Abstract Abstract. Lasiodiplodia pseudotheobromae is a cosmopolitan fungus that can cause symptoms such as leaf spot, dieback, stem rot, fruit rot and cancer which can lead to plant death. Several studies have reported that L. pseudotheobromae can infect various types of plants, including cocoa, grapes, mangoes, nuts, and papaya. The fungus L. pseudotheobromae is one of the causal agents of the cocoa dieback, which is a new disease in South Sulawesi and is considered to be one of the important diseases in the field. Fungicides are one of the methods to manage the disease on the cacao in South Sulawesi. However, the appropriate fungicides remain unexplored. Five different fungicides were used with three different concentrations, namely fipronil + methylthiofanate + pyraclostrobin (0.5- 5.0- 50) ml/liter, sulfur (0.2- 2.0- 20) gr/liter, carbendazime + hexaconazole (0.04- 0.4- 4.0) gr/liter, carbendazime (0.1- 1.0- 10) ml/liter, and diphenoconasol + azoxistobine (0.1- 1.0- 10) ) ml/liter. The fungicides was evaluated for their efficacy against L. pseudotheobromae through in vivo method. The results showed that a degree of the effectiveness of inhibition against the fungal mycelia L. pseudotheobromae. fipronil + methylthiofanate+ Piraclostrobin, carbendazime+hexaconazole and Carbendazime have excellent ability in all tested concentrations followed by recommended concentrations (2 gr / liter) and high (20 gr/liter) sulfur and high concentrations (10 ml/liter) diphenoconasol + azoxistobin. Fungicides can potentially be an option for dieback disease control in cocoa production areas that are at high risk of dieback disease in Indonesia. Keywords: Cocoa, L. pseudotheobromae, Fungicides Share Link | Plain Format | Corresponding Author (Asman Asman)

207	Integrated Pest and Disease Management	ABS-164
The Population of Solenopsis sp. (Hymenoptera: Formicidae) in Four Condition of Rice Field Bund Tamrin Abdullah (a*), Ahdin Gassa (a), Sri Nur Aminah (a), Nurul Wirid Annisaa (b) a)Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University Jalan Perintis Kemerdekaan KM. 10 Tamalanrea Makassar 90245 INDONESIA b)Magister Student of Phytopathology, Post Graduate Program, Bogor Agricultural University, Jalan Raya Dramaga Babakan, Kecamatan Dramaga, Bogor 16680, West Java, INDONESIA *E-mail: abdullah_journal84[at]gmail.com Abstract Fire red ants (Solenopsis sp.) as the predators often found in rice fields. Although many farmers not yet know well this potential insects, in Philippines, this adaptive predator has been used to control golden apple snail with satisfying results. In a period of two days, predatory ants able destroyed 50% the eggs of golden apple snail Pomacea canaliculata attached to the leaves of rice plants. The purpose of the research is to study the effect of characteristic differences vegetation of rice field bund to the diversity of ants in the habitat. Research data collection was conducted in Manggala district, Makassar. Identification and observation of ants species was held at the Pest Laboratory, Department of Plant Pests and Diseases, Faculty of Agriculture, Hasanuddin University, Makassar. The research was held from March to May 2018. The results was showed that the bund contain banana tree vegetation had the highest ant population with 288 individuals. The lowest on the bund with broad leaf grass vegetation only 29 individuals. The highest number of individual ants was found in Solenopsis sp. with 353 individuals, then the lowest is Tetramorium sp. with 2 individuals. The difference conditions of vegetation bund showed greatly affects in the number of ants due to differences in the conditions needs of each ant species for their suitable habitat. Keywords: ant, Solenopsis sp., vegetation, bund, Tetramorium sp. Share Link | Plain Format | Corresponding Author (Tamrin Abdullah)

208	Integrated Pest and Disease Management	ABS-173
Feed Finding Behavior of predatory Coccinella sp. on Kenikir Yellow Flower Syrup as its supplement Nurariaty Agus, Tamrin Abdullah dan Sulaeha Tamrin UNiversitas Hasanuddin Abstract Coccinella sp. as a predator of various kinds of pests that are cosmopolitan in nature, it is very important to be conserved. These predators need energy for their survival, which can be obtained from their prey (pests), as well as from flower nectar as a source of additional feed or supplements. In this regard, we have conducted a series of studies to make yellow kenikir flower syrup as an analogue of flower nectar. This study aims to determine the behavior of predatory feed finding Coccinella sp. to yellow kenikir flower syrup added with artificial sweetener, namely saccharin, then compared with the addition of commercial syrup. Observations were made for eight hours on the feed finding behavior including the frequency of visiting the feed and the time duration used for the feed. The results showed that saccharin the visiting frequency of Coccinella sp. adult on yellow kenikir flower syrup added with saccharin was lower but longer than if the predator was given yellow kenikir flower syrup added with commercial syrup with an index of 2.10 minutes / visit and 1.18 minutes / visit, respectively. However, the frequency and duration of predators increased in yellow kenikir flower syrup when combined with fresh yellow kenikir flowers but did not come to commercial syrup when combined with fresh yellow kenikir flowers. The yellow kenikir flower syrup added with saccharin is potent enough to be developed for the predatory Coccinella sp. as its supplements. Keywords: yellow kenikir flower syrup, supplements, predator behavior, Coccinella sp. Share Link | Plain Format | Corresponding Author (Hamdayanty Hamdayanty)

209	Integrated Pest and Disease Management	ABS-176
Effectiveness of Bacillus spp. To Increase Resistance of Cavendish (Musa acuminata) Against Blood Disease (Ralstonia syzygii subsp. celebensis) Serli, Baharuddin and Muhammad Junaid Hasanuddin University Abstract Blood disease at banana caused by Ralstonia syzygii subsp. celebensis and made financial loss to farmers, with banaas become sick or produced fruit where no one can consume it. This study aims to determine the effect of Bacillus spp. to increase resistance of cavendish against blood disease. The research was conducted in the laboratory of Agricultural Biotechnology, Department Plant Pest and Diseas Hasanuddin University. Bananas seedling was planted in the Screen House, Ex-farm of Agricultural Faculty, Hasanuddin University. The research was begun in September 2019 and was ended February 2020. In this research was used the Completely Randomized Design (CRD) method with 5 treatments and 3 replications with each treatment has 3 seedling and total of seedling in the experiment were 30 bananas seedling. Banana seedling was soaked with each 3 microbial antagonists which are PMT, PBT, PAA isolat, and 2 control (positive and nnegative). Observation has 4 steps: (1) The intensity of disease (%), (2) Height plant, (3) Number of leaves, and (4) Weight of plant. Results showed that the best treatment was found in the PMT isolat. It has potencial to be microbial antagonist to support induce of resistance better than PBT and PAA isolat treatments. PMT isolat was suppressed blood disease with percentation until 33,3%, PBT isolat oppressing blood desease until 20,83%. While, PAA isolat has potencial oppressing blood desease until 25%. Keywords: Resistance induce, Bacillus spp., Ralstonia syzygii subsp. celebensis. Share Link | Plain Format | Corresponding Author (Serli Serli)

210	Integrated Pest and Disease Management	ABS-178
TECHNICAL CULTURE TECHNOLOGY PACKAGE APPROACH TO FUSARIUM ATTACKS AND LOCAL ONION PRODUCTION IN IRRIGATED RICE FIELDS Ni Made Delly Resiani & I Wayan Sunanjaya Assessment Institute for Agricultural Technology (IAARD) Bali / Balai Pengkajian Teknologi Pertanian (BPTP) Bali Abstract Fusarium control through the cultivation of healthy plants with improved technical culture is an effective solution that can be practiced in irrigated rice fields. The aim of this study was to obtain the best technical culture technology package in controlling fusarium and increasing shallot production in irrigated rice fields. The research was conducted in Rejasa Subak Rejasa Kelod Village, Penebel District, Tabanan Regency. The research was conducted from June to September 2019. Using a randomized block design (RBD), the plots were divided into two treatment factors, namely: Factor 1, namely 2 levels of varieties, namely: local varieties of Tabanan (V1) and varieties of Bali Karet (V2). Factor 2, namely 3 levels of technology packages, namely: P1 = existing, P2 = improvement- and P3 = innovative. The parameters observed included plant height, maximum number of leaves, number of tubers, stem diameter, total dry plant weight of harvest per clump, total weight of dry plant harvest per tuber, weight of sold / stored dry tubers per clump and percentage of fusarium attack. Data were analyzed using an analysis of diversity and mean difference test. The results showed that the highest average total dry weight of harvested plants per tubers was shown in the innovative packaging (2.15) which was not significantly different from the repair package treatment (1.99) or 24.19% higher than the existing package (1.63 kg). The highest weight of selling/storing dry tubers per clump was shown in the innovative package treatment (89.48) which was 12.32 and 38.25% higher than the repair packages (78.46) and the existing (55.25 grams). The highest percentage of fusarium attacks was shown in the existing package at 36.67%, while the repair package was 13.33 and the innovative one was (16.67%). It was concluded that the application of the improved technology package and the innovative.technology gave the best results for all observed parameters Keywords: Keywords: Technical culture, fusarium, shallots Share Link | Plain Format | Corresponding Author (Ni Made Delly Resiani)

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