Identification of Fungi associated with the soil area of natural forest and post-mining area of PT Vale Indonesia
S W Jufri, A Arif, I Iswanto, S H Larekeng

Faculty of forestry, Universitas Hasanuddin

Abstract

This study aimed to identify fungi that are able to inform the microbes associated with post-mining forest soil of PT. Vale Indonesia. Here, we used the descriptive method by noting and drawing the observed phenomena. Sample collection in the field was carried out by collecting 500 g of soil per spot (three repetitions). The samples were from the rooting area or rhizosphere at 10-30 cm of depth in natural forest and reclamation areas. Isolating for growing the microbes was using the dilution technique. The purification of the obtained isolates was performed by inoculating in the PDA media with the point method, then incubated for +- five days at 27 oC and their growth was observed. Characterization of microscopic fungi isolates was identified based on the characteristics of their morphological structures. Initial research results of fungi soil samples from reclamation and natural forest areas obtained 11 rhizosphere fungi isolates. Based on the fungi colonies^ color at the top and bottom, they had various colors and different textures. The color was dominated by greenish and whitish, but some isolates had white, brown, cream, gray, yellowish, whitish, greenish, and spots. The texture was dominated by velvet, but some isolates had fine cotton and coarse cotton textures that were assumed as Aspergillus niger, Aspergillus sp, Fusarium oxysporum, Penicillium sp., Trichoderma harzianum, and Trichoderma viride. This study will be followed by validating the type and function of each isolate.

Keywords: Fungi, post-mining, identification

Topic: Plant Breeding and Biotechnology