Sterility of 20 F-1 Genotypes Derived from Hybridization of Several Chili^s Lines with M1 Male Sterile
Redy Gaswanto1*)

1) Indonesian Vegetables Research Institute, Jln. Tangkuban Parahu No. 517,
Lembang, Bandung Barat, Indonesia 40391
*)Correspondence E-mail: redwanto_1[at]yahoo.co.id

Abstract

Some open pollinated (OP) chili varieties seed already had been irradiated successfully by gamma rays (400-600 Gy) to obtain four plant individuals M1 male sterile. These sterile individuals could be used as a breeding material for F1 chili^s cyto-genic male sterile (CGMS) hybrid. The purposes was to evaluate sterility trait of 20 F1 genotypes derived from hybridization of several chili^s lines with four M1 male sterile individuals until could be categorized as candidate lines of male sterile, maintainer, and restorer. The research conducted at Indonesian Vegetables Research Institute, Lembang (1,250 m sal) from April to December 2018. The research used Randomized Complete Block Design (RCBD) with three replications. The population of each hybridization genotype was 10 plants/replication. Sterility trait evaluation was observed by fruitful ability of various flower^s grouping positions (under, middle, upper) on each hybridization genotype plant. The research result showed that there were two chili genotypes categorized as sterile lines and five genotypes as fertile lines. Male parent of sterile genotypes was a maintainer line, whereas male parent of fertile genotypes could be a restorer line candidate.

Keywords: cytoplasmic male sterile, maintainer, restorer, F-1 hybrid, gamma rays irradiation

Topic: Plant Breeding and Biotechnology