Amplification of rbcl and matK genome regions in bamboo plant species: preliminaries studies
Jeni Oktavina Kamben Patintingan, Siti Halimah Larekeng, Muh. Restu

Faculty of Forestry, Universitas Hasanuddin

Abstract

bamboo is widely used as a raw material for creative industries with economic value. About 157 species or 10% types of bamboo growing in Indonesia and 50% are endemic to Indonesia. Morphological and molecular identifications are required as the basic information for the hybridization and selection strategies. Morphological identification is performed based on morphological characters. Molecular identification can be assessed using DNA barcoding. According to the CBOL plant group, matK and rbcL are the best barcode combination for plants. In this research, we selected five matK and two rbcL primers to obtain primers that are able to amplify bamboo DNA from 2nd regional of forest seed/seedling office. Only three out of seven primers, matK 4, matK 5, and rbcL 1, could amplify the evaluated DNAs. matK 2, matK 7, matK 8, and rbcL 4 did not produce any PCR products. rbcL 1 and matK5 generated PCR amplification products, which were 700 bp in size. The size of PCR products using matK4 was 900bp. This primer amplified 15 of 16 the evaluated samples.

Keywords: bamboo, DNA barcoding, matK, rbcL

Topic: Plant Breeding and Biotechnology