POTENTIAL OVARY OF CATS BASED ON MORPHOLOGICAL NUMBER AND QUALITY OF OOCYTES IN LUTEAL AND NON-LUTEAL PERIODS Arief Boediono (a)*, Retno Saputro (a), Kusdiantoro Mohamad (a), Diah Nugrahani Pristihadi (a), Karisma Mardatillah (a), Wahyudin (a), Rini Widyastuti (a,b)
(a)Departement of Anatomy, Physiology, and Pharmacology
Faculty of Veterinary Medicine, IPB University, Bogor 16680, Indonesia
*Corresponding author: ab[at]apps.ipb.ac.id
Abstract
Ovarian waste from sterilization in female cats through ovariohysterectomy surgery can be used as a source of oocytes in an effort to produce embryos in vitro. The purpose of this study was to evaluate the potential of female ovaries after sterilization based on the number of functional oocytes successfully collected in different follicular periods, namely the luteal period and the non-luteal period. Ovary of the cat was obtained from the sterilization action through ovariohysterectomy surgery. Ovaries were distinguished based on the presence of the corpus luteum (CL) of each pair of cat ovaries, namely the ovarian pair with CL (luteal period), and ovarian pairs without CL (non luteal period). Oocytes were collected by slicing the ovarian cortex. Oocytes were grouped into functional oocytes, fragmentation, spontaneous cleavage, and degeneration. The precentage of cat ovary that has CL and without CL were 59.1 and 40.9%, respectively. There was no difference in the number of CL between left and right ovary in the luteal period group. The number of functional oocytes that can be collected in the non-luteal period (27 oocytes per head) was greater (P<0.05) than the number that can be collected in the luteal period (13 oocytes per head). The results of this study are expected to be used as additional information for the utilization of wild ovaries of the Felidae family after death through embryo production technology in vitro.
Keywords: cat, oocytes, ovariohysterektomy, ovary, luteal period
