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Histology of Medaka Sulawesi (Oryzias Celebensis) Fish Skin while Process of Puncture Wound Healing
Kadek Dian Krisna Putri K,Dwi Kesuma Sari, and Irma Andriani

Hasanuddin University


Abstract

Sulawesi Medaka fish (Oryzias celebensis) should be developed as animal models for additional information about Medaka Sulawesi because this fish is the endemic fish of Sulawesi. This study aims to observe the skin histology of the Sulawesi medaka fish during the healing process of the puncture wound. This study used the Sulawesi medaka fish which were divided into 3 groups, K1 as a control group without treatment as many as 6 fish, K2, the puncture wound treatment group of 6 fish and K3, the group of fish that were given puncture wounds then kept in the water which had been given with methylene blue. The wound healing process of K2 and K3 then observe on days 1, 3, 5, and 7. The result shows that the redness around the wound and the size of the wound decreases every day both on K2 and K3. The observations on 1st day of K2 and K3 showed that there were much inflammatory cells, fibroblasts and collagen fibers are found in few and granulation tissue were not found, while on the 3rd day there were still much inflammatory cells, fibroblasts and collagen fibers in more ways than 1st day and granulation tissue was still not found. Histological observations on 5th day of K2 showed that there were more fibroblasts, inflammatory cells were decreased, and a large amount of granulation tissue was found, while on 7th day of K2, fibroblasts were found but not as much as 3rd and 5th day with high density, inflammatory cells were also still visible, more collagen fibers were found on K2 and K3.

Keywords: histology, skin, medaka, methylene blue, Oryzias celebensis, wound

Topic: Marine Biology and Marine Disease

Plain Format | Corresponding Author (Kadek Dian Krisna Putri K)

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