Potential of Indonesian Bay Leaf (Syzygium polyanthum) as Anthelmintic Against Ascaridia galli by Scanning Electron Microscope Santika Anggrahini(a), Irkham Widiyono(b*), Soedarmanto Indarjulianto(b), Joko Prastowo(c)
a) Student of Doctoral Program Veterinery Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Bulaksumur, Sleman, Yogyakarta, 55281, Indonesia
b) Department of Veterinary Interna Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada, 55281 Bulaksumur Sleman, Yogyakarta, 55281, Indonesia
*Irkhamwidiyono[at]ugm.ac.id
c) Department of Parasitology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, 55281 Bulaksumur Sleman, Yogyakarta, 55281, Indonesia.
Abstract
Anthelmintic resistance has emerged as a major problem in parasite control, hence, it is necessary to explore the possibility of using herbal plants as an alternative to anthelmintics. This study aims to determine the potential of Indonesian bay leaves that are extracted using ethanol as an anthelmintic alternative against Ascaridia galli by employing in vitro method. Phytochemical method was used to indicate whether the extract contained the chemical being observed. In vitro method was employed to determine the effect of Indonesian bay leaf extract (BLE). Indicator was based on the resistance of worm movement which was then evaluated ultra-structurally using Scanning Electron Microscope. This study was conducted to 4 experimental groups, consisting of 3 BLE treatment groups with varying degrees of solution concentrations (2%, 8% and 14%) and a negative control group. The results showed that A. galli in all groups did not die during the 10 hours immersion. BLE up to a concentration of 14% can damage the cuticle of A. galli. Based on this experiment, it was concluded that BLE is potential to be an anthelmentic against A. galli. Further research is then need to be conducted to determine the ideal dosage.
Keywords: Syzygium polyanthum, Ascaridia galli, in vitro, SEM
