The Alteration of Short-Time Incubation of Extreme pH Media to the Morphology of Freeze-Thawed Pasundan Bull Sperm Rini Widyastuti(a,b), Hera Maheswari (a), Cece Sumantri (c), Diah Nugrahani Pristihadi (a), Noer Muhamad Dliyaul Haq (a), Wahyudin(a), Arief Boediono(a*)
(a) Departement of Anatomy, Physiology, and Pharmacology,Faculty of Veterinary Medicine, IPB University. Jl. Agatis Dramaga Bogor,
West Java 16680, Indonesia
*ab[at]apps.ipb.ac.id
(b)Laboratory of Animal Reproduction and Artificial Insemination, Departement of Animal Production, Animal Husbandry Faculty,Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km.21 Jatinangor Sumedang, West Java 45363, Indonesia
(c) Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Abstract
The present study aimed to evaluate the morphology of freeze thawed Pasundan bull sperm after short-time exposure to extreme acidic and alkali media. The freeze thawed Pasundan bull sperm were centrifuged for 500g for 5 minutes to remove the cryoprotectant. The sperm pellet was added with buffer media and aliquoted to 25 million sperm/ml. The extreme acidic and alkali media were prepared by addition of HCL or NaOH to buffer solution until reaches the pH 4 and 11. The buffer media was used as a control media. Each sample was incubated for 5 minutes with a specific pH media at room temperature, then add a buffered media until reach a pH 7. The evaluation of sperm morphology was performed after 15 minutes of incubation at room temperature. The results showed that the percentage of sperm with normal morphology significantly decreased after incubation at acidic and alkali media compared to control. Interestingly, the short time incubation in extreme acidic and alkali media significantly increased the percentage of sperm with major abnormal morphology, mainly abnormal head shape and abnormal acrosome compared to control. While the minor abnormal sperm defect was only affected by extreme acidic media. It can be concluded that the short period incubation in an extreme acidic or alkali media significantly induced a deleterious effect to morphology of freeze thawed Pasundan bull sperm although immediately returned to normal a physiologic condition.
