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Identification of Tissue Changes in Shrimp Vaname (Penaeus vannamei) Infected with White Spot Syndrome Virus
A. Fadlillah Nal Azkina, Hilal Anshari, Dwi Kesuma Sari, A. Magfira Satya Apada

Veterinary Medicine Study Program, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar, South Sulawesi, 90245, Indonesia


Abstract

Vaname shrimp is one of the shrimp that has economic value and is an alternative type of shrimp that can be cultivated in Indonesia, besides tiger shrimp (Panaeus monodon) and white shrimp (Panaeus merguensis). The purpose of this study was to determine the histological picture of tissue changes that occur in shrimp vaname (Penaeus vannamei). The samples used were vaname shrimp collected and then prepared, afterwards the samples were extracted using DTAB-CTAB, then continued amplified using conventional PCR machines, where the first step PCR was first then Nested PCR was added. Samples that had been amplified were then electrophoresis with a voltage of 150V for 30 minutes then read results. Gills and hepatopancreas samples were then taken for histological preparations. Preparation is done by method with a sample processed in tissue processing then embed and blocked with paraffin then given hematoxylin eosin (HE) staining and analyzed descriptively qualitatively. PCR test results obtained different results in each organ, as shown in the swimming leg organ with the appearance of 3 (three) DNA bands / bands parallel to the marker (910 bp, 550 bp and 296 bp), which means the swimming leg organ is infected with WSSV the level of severe (severe) with WSSV virus infection is 2000 copies / reaction, whereas in the gill organs the emergence of DNA bands / bands are only 2 (two) bands parallel to the 296 bp and 550 bp markers indicating that the organ is infected with the WSSV virus at medium level with WSSV virus infection is 200 copies / reactions and hepatopancreas organs that show negative results with only the appearance of a smear. Histology test results showed a tissue change in the form of inclusion body and necrosis of the gills and hepatopancreas.

Keywords: Histology, PCR, Vaname Shrimp, White Spot Syndrome Virus (WSSV)

Topic: Marine Biology and Marine Disease

Plain Format | Corresponding Author (A Fadlillah Nal Azkina)

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