Antibiotic susceptibility of lactic acid bacteria isolated from duck and Jawa super chicken excreta intended for use as probiotic Dini Dwi Ludfiani (1), Widya Asmara (2*), Agnesia Endang Tri Hastuti Wahyuni (2), and Pudji Astuti (3)
1) Student in Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna Number 2, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia 55281
2) Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna Number 2, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia
3) Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Fauna Number 2, Caturtunggal, Depok, Sleman, Yogyakarta, Indonesia
*) Corresponding author: wied_as[at]ugm.ac.id
Abstract
Lactic acid bacteria (LAB) have many reported to be probiotics for animals and humans. But to assure their safety in feed or food, antibiotic susceptibility test is necessary as a precautionary measure that should be taken to protect animals, humans, and the environment from potentially unsafe microorganisms (cause resistance). The aim of this study was to determine the antibiotic susceptibility of LAB strains that isolated from duck and Jawa super chicken excreta that were collected from Sleman, Yogyakarta. The LAB isolates were identified phenotypically based on morphology, physiology, and biochemical characteristics. Antibiotic susceptibility test using the disc diffusion method. Five antibiotics were commonly used for the treatment of infections in animals and humans, which were ampicillin (10 ug), bacitracin (10 ug), erythromycin (15 ug), streptomycin (10 ug), and tetracycline (30 ug). The results showed that all isolates were identified as Lactobacillus spp. (41.67% L. brevis and 58.33% L. pentosus). All isolates showed different resistance to antibiotics. A total of 91.67% LAB isolates were resistant to streptomycin and 4 isolates were found resistant to all antibiotics. One LAB isolate from duck excreta was found sensitive to three antibiotics (ampicillin, erythromycin, and tetracycline) and it could possibly be used for probiotic.
Keywords: Antibiotic resistance, lactic acid bacteria, duck excreta, Jawa super excreta
