Detection and Identification of Aphelenchoides fragariae nematodes on Shallot Bulbs in Bogor, West Java, Brebes Central Java and Nganjuk, East Java a) Graduate School of Department of Plant Protection, Faculty of Agriculture, IPB University, Bogor. Indonesia Abstract Shallot is one of the important horticultural commodities in Indonesia. Aphelenchoides fragariae is the major parasitic nematode of shallots and listed as quarantine pest with limited distribution. The nematode was detected on shallot bulbs at several traditional markets in Bogor but has not been reported present on shallot plantation. This research aims to detect and identify A. fragariae on shallot bulbs. Shallot bulbs were sampled from Bogor, West Java, Brebes, Central Java, and Nganjuk, East Java. Nematodes were extracted from shallots bulb by cold water immersion method at 20 oC. Morphological identification was carried out by following the standard taxonomic for the Aphelenchoides genus (Aphelenchoididae). Morphometric indications were based on the de Man^s formula. The result showed that A. fragariae were found on shallot bulbs from Bogor, Brebes and Nganjuk. The female of A. fragariae has a slender body and off-set lip characteristic distinguishes it from other nematodes. The stylet was invisible, but the metacorpus (median bulb) was visible and full rounded, tail end with single mucro. The morphometric A. fragariae are n=40, L = 312.2 - 550.5, a = 20.0 - 33.9, b = 7.0 - 12.0, c = 3.9 - 15.2, V = 34.4 - 84.4, T = 28.8 - 121.3, stylet 6.8 -12.0. Keywords: foliar nematode, morphology, morfometric, parasitic nematode Topic: Integrated Pest and Disease Management |
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