The effect of several fungicides against Lasiodiplodia pseudotheobromae isolated from cacao through in vitro test
Musdalifa1, Asman Asman1,2*, Ade Rosmana1,2

1 Department of Plant Pests and Diseases, Hasanuddin University, South Sulawesi, Indonesia
2 Cocoa Research Group (CRG), Faculty of Agriculture, Hasanuddin University, South Sulawesi, Indonesia


Abstract

Abstract. Lasiodiplodia pseudotheobromae is a cosmopolitan fungus that can cause symptoms such as leaf spot, dieback, stem rot, fruit rot and cancer which can lead to plant death. Several studies have reported that L. pseudotheobromae can infect various types of plants, including cocoa, grapes, mangoes, nuts, and papaya. The fungus L. pseudotheobromae is one of the causal agents of the cocoa dieback, which is a new disease in South Sulawesi and is considered to be one of the important diseases in the field. Fungicides are one of the methods to manage the disease on the cacao in South Sulawesi. However, the appropriate fungicides remain unexplored. Five different fungicides were used with three different concentrations, namely fipronil + methylthiofanate + pyraclostrobin (0.5- 5.0- 50) ml/liter, sulfur (0.2- 2.0- 20) gr/liter, carbendazime + hexaconazole (0.04- 0.4- 4.0) gr/liter, carbendazime (0.1- 1.0- 10) ml/liter, and diphenoconasol + azoxistobine (0.1- 1.0- 10) ) ml/liter. The fungicides was evaluated for their efficacy against L. pseudotheobromae through in vivo method. The results showed that a degree of the effectiveness of inhibition against the fungal mycelia L. pseudotheobromae. fipronil + methylthiofanate+ Piraclostrobin, carbendazime+hexaconazole and Carbendazime have excellent ability in all tested concentrations followed by recommended concentrations (2 gr / liter) and high (20 gr/liter) sulfur and high concentrations (10 ml/liter) diphenoconasol + azoxistobin. Fungicides can potentially be an option for dieback disease control in cocoa production areas that are at high risk of dieback disease in Indonesia.

Keywords: Cocoa, L. pseudotheobromae, Fungicides

Topic: Integrated Pest and Disease Management

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