Resistance Validation of Several Chili Pepper Lines to Anthracnose Colletrothicum acutatum Isolate Sukabumi Neni Gunaeni1, Eli Korlina, Redy Gaswanto
Indonesian Vegetables Resarch Institute
Jln. TangkubanPerahu No. 517 Lembang-Bandung Barat (40391)
*Correspondence E- mail: nenigunaeni63[at]gmail.com
Abstract
Anthracnose is the most dominant disease that infects to chili pepper and it can reduce yield. One technique to control the disease is using resistant variety. The objective research was to ensure resistance level of the screened chili pepper lines to C. acutatum as the most virulent pathogen. The research was conducted at Indonesian Vegetables Research Institute from June to December 2019. C. acutatum isolate Sukabumi was used as an inoculum. This isolate had a DNA band 490 bp according to PCR identification. The used screening method was artificial in the laboratory by inoculating 5 ul of conidial suspension with a density of 5.0 x 105 per one injection per fruit. PR protein analysis was performed on infected fruit and healthy. The selected lines results obtained that 2A (R-4), 4A (R-15), 4B (R-9), 6 (R-12), 7 (-13 ), 8 (R-14) were resistant to anthracnose C. acutatum isolate Sukabumi. The protein band pattern profile by PR-protein analysis showed that a gene expression with molecular weight around 37 kDa.
Keywords: Capsicum annuum, Colletrotrichum acutatum, Chili pepper lines, Isolate Sukabumi
