Protein Profiles of Seminal Plasma and their Correlation with Semen Quality in Aceh Bull Hafizuddin (a*), Teuku Zahrial Helmi (a), Ginta Riady (a), Husnurrizal (a), Muslim Akmal (a), Zul Azmi (b), Yusmadi (c, d), Muhtar (d), Jauhari (d), Munzir (d)
a) Faculty of Veterinary Medicine Universitas Syiah Kuala, Jl. Teungku Hasan Krueng Kalee No.4, Banda Aceh 23111, Indonesia
*hafizuddin_umar[at]unsyiah.ac.id
b) Indonesian Research Center for Veterinary Sciences, Jl. RE. Martadinata Haji Bin Ali No.30, Kota Bogor 16124, Indonesia
c) Department of Animal Science, Faculty of Agriculture, Almuslim University, Jl. Jalan Kampus Timur, Matangglumpangdua, Bireuen 24261, Indonesia
d) Indrapuri Livestock Breeding and Forage Centre, Jl. Banda Aceh - Medan KM. 24, Indrapuri, Aceh Besar 23373, Indonesia
Abstract
This study aims to characterize the protein profile of seminal plasma in Aceh bulls and to determine its correlation with semen quality. A total of four bulls (24-34 months) belonging to the Indrapuri Livestock Breeding and Forage Center were selected randomly. An aliquot (1 ml) from each ejaculation was used for the evaluation of semen quality (semen volume, mass motility, sperm motility, sperm viability and sperm morphology). The remaining volume of semen was centrifuged to obtain seminal plasma. Total seminal plasma protein concentration was calculated using Bradford method, then the seminal plasma protein was electrophoresed on SDS-PAGE and visualized with coomasie blue. The results of protein visualization found 16 protein bands with different molecular weights, ranging from 11 to 180 kilo Dalton. In general, the protein band of 15.24 kilo Dalton was more prominent in Aceh bulls. In addition, the seminal plasma protein concentration showed a positive correlation with sperm motility, sperm viability, and sperm morphology. In conclusion, the seminal plasma protein of Aceh bulls is positively correlated with several semen quality variables and may be a useful as additional parameter for determining semen quality or bulls fertility.
Keywords: Protein profiles, Seminal plasma, Semen characteristics, Aceh cattle, Bull fertility
