Systemic and Local Expression of Endothelin-1 Lead to Early Renal Impairment In High Fat Induced Wistar Rats Haslindah Dahlan(1), Muhammad Husni Cangara(1), Rina Masadah(1), Irfan Idris(2), Upik A Miskad(1), Andi Alfian Zainuddin(3), Miswani Syuaib(1), Takao Fujimura(4)
1Department of Pathology Anatomy, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
2Department of Physiology, Faculty of Medicine Hasanuddin University, Makassar Indonesia
3Department of Public Health, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
4Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
Abstract
The pathogenesis of chronic kidney disease in obesity is being suspected of having a strong relationship with the role of Endothelin-1 (ET-1). This study aims to assess levels of expression ET-1 in serum and renal tissue associated with changes in kidney structure. The rats were divided into two groups. The group that was given a high-fat diet and the control were assigned a standard diet. The serum of blood checked for ET-I using ELISA methods and saw the distribution of ET-1 expression by Immunohistochemistry (IHC) and Periodic Acid Schiff (PAS) staining from the paraffin-blocked kidneys. ET-1 levels in the serum of high fat-induced rat groups were higher than the control (p<0,05) as well as in glomeruli of the renal tissue (p<0,05). PAS staining enhancement of basal expansion is higher in High Fat induced rats than the control (p<0,05). These data confirm the role of ET-1 will provide changes in the levels of tissue and serum levels in obesity di early kidney injury.
Keywords: Endothelin-1, obesity, early kidney injury, chronic renal failure
