MERCURY CHLORIDE (HGCL2) AFFECTS THE ANATOMICAL PATHOLOGY AND HISTOPATHOLOGY OF MALE TILAPIA (OREOCHROMIS MOSSAMBICUS) GONAD Nazaruddin1, Dwinna Aliza1, Teuku Armansyah2, Roslizawaty3, Erdiansyah Rahmi4, Nuzul Asmilia3, Putika Sari Ayu5
1Pathology Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh
2Pharmacology Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh
3Clinical Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh
4Histology Laboratory, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh
5Veterinary Medicine Study Program, Veterinary Medicine Faculty, Universitas Syiah Kuala, Banda Aceh
Corresponding author: Dwinna Aliza, email: dwinna.aliza[at]unsyiah.ac.id
Abstract
In fishes, Hg can inhibit gametogenesis, induce testicular atrophy, and impair individual reproduction. Despite some of Hg induced damages are known, there is still less information about the metal accumulation on tissue and its relationship with tissue structure disorganization of the fish testis, especially for tropical species. This study aimed to determine the effect of HgCl2 exposure on anatomical pathology and histopathology of male tilapia fish (Oreochromis mossambicus) gonad. A total of 36 male tilapia fish were allotted into 4 treatment groups with 3 replications. Fish were exposed to 0.00, 0.25, 0.50, and 0.75 ppm of HgCl2 for 10, 20, and 30 days. Fish gonad appearance was observed followed by histopathological examination using hematoxylin and eosin staining. The anatomical pathology examination of fish gonad demonstrated several changes from atrophy, hypertrophy, to necrosis. The histopathological observation showed that the lowest averages of spermatozoa spreading in seminiferous tubule were shown in group K4, and the highest average of gonad cells necrosis number was observed in group K4 with 30 days exposure. Statistically, both histopathology parameters were significantly different compare to the control group. In conclusion, HgCl2 exposure changes the anatomical pathology and histopathology of male tilapia fish gonad. The higher the HgCl2 concentration and the longest time of exposure the more severe the damage of the fish gonad.
Keywords: mercury, fish male gonad, histopathology, necrosis, athrophy
