The effect of time interval differences between the prostaglandin f2 alpha injection and semen collection on the improvement of spermatozoa and testosterone concentrations in Aceh Bull BUDIANTO PANJAITAN, VALERIE YOPPY EDWARD, DWI ATMAJA NISHCAYA, HUSNURRIZAL HUSNURRIZAL, TEUKU ARMANSYAH, MUSLIM AKMAL, TONGKU NIZWAN SIREGAR, HERRIALFIAN
Faculty of Veterinary Medicine, Universitas Syiah Kuala, Aceh, Indonesia
Abstract
The injection of prostaglandin F2 alpha (PGF2\alpha) has been adopted as an alternative to improve sperm quality, and the difference in sample collection time after administration is assumed to have a positive effect on testosterone concentration. This research was aimed at determining the effect of PGF2\alpha injection and the optimal time for sample collection on improving semen quality and testosterone concentrations in Aceh bull. Aceh bull was used in this study and allotted into 2 treatments. In treatment 1 (P1), the bull received 25.0 mg PGF2\alpha (Lutalyse, dinoprost, thromethamin) 1 hr prior to semen collection, while in treatment 2, the semen collection was carried out 2 hrs after PGF2\alpha administration. The treatment was performed five times on alternate weeks Semen collection was performed using an artificial vagina once a week for ten week Testosterone concentrations were analyzed using the enzyme linked immunosorbent assay (ELISA) method. The average spermatozoa volume (mL), concentration (106 cells/mL), and testosterone concentration (ng/mL) in P1 vs P2, were 4.82\pm1.93 vs 4.92\pm2.45, 889.0\pm250.50 vs 721.0\pm246.25 (P<0.05), and 8.35\pm2.34 vs 9.60\pm2.24 (P>0.05), respectively. It is concluded that the PGF2\alpha injection 1 hour prior to collection was able to increase the spermatozoa concentration compared to the 2 hours treatment, while the value obtained for testosterone was not affected.
