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Description of histochemical of gills and kidney betok fish (Anabas testudineus Bloch, 1792) at Tunggu Pampang Makassar reservoir 1. Study Program of Veterinary Medicine, Faculty of Medicine, Hasanuddin University, Jl. Perintis Kemerdekaan. KM. 10, Makassar, 90245, Indonesia Abstract Histochemistry is the study of tissue staining in histological staining. This study aims to analyze histological structures specifically with histochemical techniques of gill and kidney fish (Anabas testudineus) in reservoir Tunggu Pampang Makassar. The research method is experimental descriptive. This research was conducted in August - September 2018 at the Animal Clinical Pathology Laboratory of Hasanuddin University Education, using a sample of 6 Anabas testudineus fish which have a length of 12-13.5 cm with an average of 12.6 cm and a weight size of 28 , 9 - 29.6 g with an average of 29 g of fishermens catch in the waters of the Tunggu Pampang reservoir. Histological preparations were made using the paraffin method and histochemical staining, namely Hematoxylin-Eosin (HE), alcian blue (AB) periodic acid schiff (PAS). The results showed a difference in the results of staining on the gills and kidneys of betok fish. Hematoxylin - eosin (HE) staining shows the histological structure of gills and kidneys. The coloring of Alcian blue (AB) pH 2.5 in the gills and kidneys of betok fish shows the concentration of distribution of acidic carbohydrates marked in blue which shows a positive reaction. Meanwhile, staining of Periodic Acid Schiff (PAS) shows a positive reaction to the gills and kidneys which are characterized by the formation of mangenta color. Keywords: Alcian blue, kidney, hematoxylin - eosin, histochemistry, histology, gills, Periodic Acid Schiff. Topic: Veterinary Clinic, Reproduction, Pathology |
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