Molecular Analysis of BMP 15 Gene in Sumba Ongole Cattle, Sumba Island, East Nusa Tenggara Cynthia Dewi Gaina1*, Maxs U. E.Sanam1, Marlene WM Nalley2, Imanuel Benu2, Agus Saputra1
1 Faculty of Veterinary Medicine, Nusa Cendana University, Indonesia
2 Faculty of Animal Science, Nusa Cendana University, Indonesia
Abstract
Sumba Ongole cattle are one of local beef cow in Indonesia that has been known for its benefit as source of protein and highly adaptive to extreme climate, like Sumba Island. Many genes are known to have an effect on reproductive performances traits, but the role of one gene, BMP 15 has not been explored yet. This gene is responsible for regulating ovarian function in cows. Due to the rare information about this gene characteristic, this study aimed to identify BMP 15 gene in Sumba Ongole (SO) cattle and its functions on reproduction system. The blood sample from 47 SO cattle which reared extensively in Sumba Island, were collected and were analyzed. The method used in this research was polymerase chain reaction (PCR), electrophoresis and DNA Sequencing. All DNA samples were amplified using BMP15 primer for 35 cycles and amplicons were visualized on 1.5% agarose gel. A total of 47 samples have been sequenced in PCR machine. The BMP 15 gene forward primer was 5^-AGTTTGTACTGAGCCGGTCT-3^ and reverse primer was 5^-CTGACACACGAAGCGGAGT-3^. The software Basic Alignment Local Search Tool (BLAST) was used to sequence and to analyses. The results revealed that the size of all the fragments was in accordance with that of BMP 15 and it was not found any genetic variations among the samples.
