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1	Animal Agribusiness and Related Subject	ABS-34
The consumer acceptance of novel foods on potential markets using an extended behavior model Awang Tri Satria 1,2, Jaisy Aghniarahim Putritamara 2 1. Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology, Pingtung 912, Taiwan 2. Department of Socio-Economic, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract This research aims to determine the purchase intention of cultured meat using an expanded planned behavior theory approach. The development of science has encouraged scientists to look for the latest innovations known as novel foods with the aim of alternative food in the future. Research on consumer acceptance of cultured meat has been carried out in many developed countries by looking at the potential for various consumer acceptance of cultured meat. Indonesia, as one of the potential markets for novel foods, is necessary to also look at the potential for consumer acceptance of novel foods, especially on cultured meat. This research used an online survey method on 231 young respondents in Indonesia. The results show that constructive attitudes and subjective norms significantly affect the purchase intention of cultured meat. Environmental beliefs also significantly affect the purchase intention of cultured meat. The results of this research can be used as a reference for marketing strategies for cultured meat in the future, especially in the young age segment in potential markets in developing countries Keywords: Consumer acceptance- Novel foods- Indonesian consumers- cultured meat- Potential foods Share Link | Plain Format | Corresponding Author (Awang Tri Satria)

2	Animal Agribusiness and Related Subject	ABS-54
^Egg sell points^ an chicken eggs marketing strategy based on smart farming system Dian Khofifah Manurung 1- Richi Dwi Firmansyah 1- Awang Tri Satria 2- Jaisy Aghniarahim Putritamara 2 1- Undergraduate student of the Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia 2- Lecturer at Department of Socio-economic Faculty of Animal Science, Universitas Brawijaya, Malang, Indonesia Abstract Post-COVID-19 in 2022, reported an increase in chicken egg consumption by 2.7 percent. In 2021, the amount was 18.92 Kg/Capita/year to 20.02 Kg/capita/year in 2022. This condition is an excellent opportunity for laying hen farmers to maximize productivity and profits. However, laying hen farmers still carry out traditional cultivation, need to implement smart farming systems in the production process, and rely on one marketing channel. The first objective of this study is to overview the potential of laying hen farms using a smart farming system approach with the aim of farmers being able to diversify products with segmented distribution channels. The second is to build a segmented marketing network according to the product needs of each consumer. The expected result of this study is that farmers can maximize productivity and increase profits through segmented distribution channels. The innovation of this marketing system will be called ^Eggs sell points^ an integrated chicken egg marketing system through a sales point connected to the Internet of Things. Keywords: Innovation- marketing strategy- Egg sell points- smart farming- innovative farmer Share Link | Plain Format | Corresponding Author (Awang Tri Satria)

3	Animal Agribusiness and Related Subject	ABS-57
Formulating a Strategy for Development of Smallholder Beef Cattle Farming in Indonesia with the Force Field Analysis (FFA) Method Amam Amam (a*), Mochammad Wildan Jadmiko (a), Pradiptya Ayu Harsita (a), Supardi Rusdiana (b) a ) Department of Animal Husbandry, Faculty of Agriculture, Universitas Jember b) National Research and Innovation Agency (BRIN), Indonesia Abstract Beef cattle farming in Indonesia is dominated by smallholder livestock farming on a micro scale, namely less than 5 (five) heads, raised traditionally with limited resources, but is sustainable. This research aims to formulate a strategy for developing smallholder livestock farming. Research data was obtained by means of Focus Group Discussion (FGD), filling out questionnaires, and in-depth interviews with key informants. Data analysis uses the Force Field Analysis (FFA) method which is based on driving factors (D) and inhibiting factors (H). The research results show that the driving factors for the development of smallholder beef cattle farming in Indonesia are consumption needs and market availability, government policy and institutional support, social and cultural community, availability of animal feed sources, availability of upstream and downstream industries, as well as regional carrying capacity and appropriate technology, while the factors inhibiting the development of smallholder beef cattle farming in Indonesia are that livestock ownership is not yet fully focused on business, livestock business is used as family savings, market access and long marketing chains, limited resource accessibility, poor reproductive management and low availability of feeders, as well as weak protection of the selling price of live cattle and it tends to fluctuate. The research conclusion shows that the driving factor (D) has a smaller value when compared to the inhibiting factor (H), so the proposed policy recommendation is to strengthen government policies and institutional support oriented to the agribusiness sector (upstream, onfarm and downstream) and agro-industry. Keywords: beef cattle, smallholder farming, ruminant, livestock business Share Link | Plain Format | Corresponding Author (Amam Amam)

4	Animal Agribusiness and Related Subject	ABS-58
Driving Factors for Sustainable Livestock Development in Indonesia: Study on Beef Cattle Commodities a) Amam Amam, b) Muchamad Luthfi, c) Koko Wisnu Prihatin, d) Tian Jihadhan Wankar a) Department of Animal Husbandry, Faculty of Agriculture, Universitas Jember, Indonesia b) National Research and Innovation Agency (BRIN), Indonesia c) Balai Besar Inseminasi Buatan (BBIB) Singosari, Indonesia d) Department of Socioeconomic Animal Husbandry, Faculty of Animal Science, Gadjah Mada University, Indonesia Abstract The driving factors for the development of smallholder beef cattle farming include consumption needs and market availability, government policy and institutional support, social and cultural community, availability of animal feed sources, availability of upstream and downstream industries, as well as regional carrying capacity and use of appropriate technology. The aim of the research is to analyze the influence of driving factors on sustainable livestock development in beef cattle commodities. The research variables consist of driving factors (X) and the five dimensions of sustainable livestock development, namely the ecological dimension (Y1), the economic dimension (Y2), the social and economic dimension (Y3), the institutional dimension (Y4), and the technological dimension (Y5). Research data was obtained by means of Focus Group Discussion (FGD), filling in questionnaires, and interviews. The research respondents consisted of 60 small-scale beef cattle fattening farmers with ownership of 7-60 heads in Bondowoso Regency. Data were analyzed partially using simple linear regression with SPSS 26.0. The research results showed that driving factors had a positive and significant influence on sustainable livestock development, especially on the ecological dimension of 0.492, the economic dimension of 0.376, the social and cultural dimension of 0.327, and the institutional dimension of 0.442. The research conclusion shows that the driving factors for sustainable livestock development are worthy of the attention of all stakeholders in beef cattle farming commodities. Keywords: beef cattle, livestock business, ruminants, sustainable livestock development. Share Link | Plain Format | Corresponding Author (Amam Amam)

5	Animal Agribusiness and Related Subject	ABS-66
Influence of Forage Supply, Concentrate Provision, and Farmer Characteristics with the Number of Cattle in Breeding Village Putut Suryo Negoro (a,c), M Nasir Rofiq (b), and Nur Rochmah Kumalasari (a) a) Study Program of Feed Nutrition Science, Department of Nutrition and Feed Technology, Faculty of Animal Science, IPB University b) Research Centre for Sustainable Production System and Life Cycle Assessment National Research and Innovation Agency c) Research Center for Animal Husbandry National Research and Innovation Agency Abstract This study aimed to investigate the influence of forage supply, concentrate provision, and farmers^ characteristics on the number of cattle in the breeding village. One hundred and three farmers were selected, in interviews were carried out aiming to characterize the management practices related to forage supply, concentrate supply, and farm labour. A linear model was performed to analyze the significant variables. The result showed that the number of cattle was influenced by forage stock, farmers^ experience, and occupation. the correlation analysis showed a weak correlation between forage stock and farmer experience with the number of cattle. The clustering showed the direction effect of forage stock to the right side while the farmers^ experience to the bottom of the cluster. It concluded that the forage supply and farmers^ experience have influenced the number of cattle. Keywords: farmers characteristics, forage supply, cattle breeding village Share Link | Plain Format | Corresponding Author (Putut Suryo Negoro)

6	Animal Agribusiness and Related Subject	ABS-68
Production Costs Analysis for Madura Cattle Farming in Tampojung Village, Waru District, Pamekasan Regency ubmit This Sample Abstract Kuswati(1), Rizki Prafitri(1), Hermanto(1), Onni Meirezaldi(2), Herlina Pratiwi(3), Roudhotul Febry Utami(1), Zahrah Mufidah(1) Universitas Brawijaya Abstract This research aims to analyze the production costs of raising Madurese cattle for smallholder farming with average ownership of 1-3 cattle per farmer incurred by farmers. The research location was chosen because Tampojung Guwa Village is one of the areas in the PAPABARU area (Pasean, Pakong, Batu Marmar, and Waru), which has innovations in developing Madura cattle breeding. Farmers in these areas pay attention to maintaining genetic purity. Therefore, the government expects these areas to become the basis for Madura cattle. The survey method collects data through interviews with Madurese cattle breeders in Tampojung Guwa Village using a questionnaire tool. The data analysis method was carried out descriptively and quantitatively. The research results obtained production costs, revenues, R/C ratio, and profitability of Madurese cattle farms for respective livestock ownership. This research indicated that the Madurese cattle farming business is profitable for farmers. Ownership of 3 heads equals Rp. 51,203,000, receipts reached Rp. 60,000,000, R/C ratio 1.17/breeder/year, and profitability 17%- ownership of 2 heads of production costs Rp. 33,082,000, receipts Rp. 40,000,000 with an R/C ratio of 1.21/breeder/year, profitability of 21%, and ownership of 1 head production costs Rp. 16,708,000, receipts Rp. 20,000,000, R/C ratio 1.20/breeder/year, and profitability of 20%. Keywords: production costs, Madura, Cattle, Pamekasan Share Link | Plain Format | Corresponding Author (Rizki Prafitri)

7	Animal Nutrition	ABS-1
The use of R Programming Language for modelling in vitro Rumen fermentation gas production H. Herdian a), A. Sofyan a), H. Kusnadi a), A.S. Anggraeni b), I. N. G. Darma c) a) Research Center for Animal Husbandry National Research and Innovation Agency (BRIN), Cibinong Indonesia b) Research Center for Food Technology and Processing National Research and Innovation Agency (BRIN), Gunungkidul Indonesia c) IPB University Bogor Indonesia Abstract For feed evaluation involving the microbial fermentation process in the rumen by the in vitro technique, a program script has been made using the R programming language to carry out the fitting data process of gas production observation from rumen fluid fermentation. The calculation process uses nonlinear mathematical equations from Orskov and McDonald (1979). The equation shows the gas production (P) (mL) is influenced by several constants that indicate the share of gas production from the soluble fraction (FA) (mL), gas production from the potentially soluble part (FB) (mL/h), gas production rate (KC) (mL/h) and incubation time (T) (h), in addition to P and T, the three constants are tried to be derived by the program created. The data set was taken from the study of (Sofyan, et al 2023) and (Anggraeni, et al, 2022). The Neway Software program of curve fitting calculations from Chen (1997) was used to validate the results, as the mean difference was tested using an unpaired T-test. Both programs showed the same calculation results for FA (P=0.9996), FB (P=0.9998), and KC (P=0.9962). The residual standard error (RSE) and R-square (R2) values of both programs also came with the same result they were RSE (P =0.9978 ), and R2 (P=0.6087) respectively. This shows that the R program script made can carry out the calculation process of making a model of the gas production equation for modeling in vitro gas production from rumen fermentation. Keywords: In vitro, rumen gas production, R language program Share Link | Plain Format | Corresponding Author (Hendra Herdian)

8	Animal Nutrition	ABS-3
Effect of Addition Fermented Herbal Dregs as Feed Additive on Quality External of Quail (Coturnix coturnix Japonica) Devita Amelia (a), and Osfar Sjofjan (b) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *dvtmly_[at]student.ub.ac.id b) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract The purpose of this study was to determine the effect of the addition fermented herbal dregs on the external quality of quail eggs. This material used 200 quails (Coturnix coturnix japonica) starting from 35 days old quail given the addition of herbal dregs as a feed additive with different feeding composition. Maintenance time and data collection were carried out for 45 days. The methods were conducted experimentally using the complete randomized design (CRD) method with 5 treatments and 4 replicates to determine the effect of the addition herbal dregs fermented feed additives on the external quality of quail eggs. The treatments implemented differed only in the levels of herbal dregs mixed with basal feed T0 = Control Treatment, T1 = Basal feed + 0.25% fermented herbal dregs, T2 = Basal feed + 0.50% fermented herbal dregs, T3 = Basal feed + 0.75% fermented herbal dregs, P4 = Basal feed + 1.0% fermented herbal dregs. The variables measured were egg weight, egg length, egg width, egg index, specific gravity, and quail egg shell thickness Data were analyze by complete random design and DMRT. The results showed that the addition of fermented herbal pulp did not give a significant effect (P>0.05) on egg weight, egg length, egg width, egg index, egg gravity, and egg shell thickness. The use 1,0% herbal dregs fermentation give the best effect of external quality of quail. Keywords: Quail, Feed Additive, Fermentation of Herbal Dregs, External Quality of Eggs Share Link | Plain Format | Corresponding Author (Devita Amelia)

9	Animal Nutrition	ABS-5
Synthesis of selenium conjugated insects protein in Hermetia illucens larvae as poultry feed. David Kurniawan (a), Eko Widodo (a), Agus Susilo (a), and Osfar Sjofjan (a*) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *osfar[at]ub.ac.id Abstract Selenium (Se) is an essential trace element for human health and livestock growth. The element can be supplemented to feed in inorganic or organic Se forms, where the chemical form in the diet affects the accumulation of Se in animal tissues. Insects are known to be natural bio-accumulators of various nutrients, but research on the capacity of insects to be bio-enriched with Se in feed is still limited. The aims of this study were to synthesize Se-enriched insects and examine how effectively they can deliver selenium to black soldier fly (BSF, Hermetia illucens) larvae. In this study, BSF larvae were reared on different substrates with four levels of dietary sodium selenite supplementation (0, 100, 200 and 400 mg/kg). BSF larvae were analyzed for biomass yield, nutritional value and Se content. We revealed that selenium content in the fortified BSF larvae was several orders of magnitude higher than in the unfortified ones. There were significant differences between doses of selenium delivered to BSF larvae by different selenium compounds. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis of these BSF larvae has shown that there was a higher accumulation of total selenium content in the treated samples (348 mg Se/g DW) as compared to control (0.64 mg Se/g DW). Organic Se-enriched can with stand as high as 400 mg sodium selenite without compromising its growth pattern and BSF larvae biomass yield and nutritional value. Collectively, the current study proposes that, Se-enriched BSF larvae with high organic Se content can serve as selenium feed additives. Keywords: Organic Selenium, Nutritional Value, Feed Additive Share Link | Plain Format | Corresponding Author (David Kurniawan)

10	Animal Nutrition	ABS-11
The Evaluation of Indigofera Seed Production and Quality in Different Environments Elly Dianita Sari (a,b*), Luki Abdullah (a), Nur Rochmah Kumalasari (a), Achmad Fanindi (c) a) Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University, Indonesia b) Directory of Feed, Directorate General of Livestock and Animal Health Services (DGLAHS) Ministry of Agriculture Indonesia c) Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), Cibinong, Indonesia Abstract Seed production and quality are pivotal factors in the success of Indigofera cultivation as forage crop for livestock. This study aims to evaluate seed production and quality of Indigofera in different environments of Seed Production Unit (SPU). Seven distinct SPUs namely BPPIB TSP Bunikasih, BBIB Singosari, BPTU HPT Denpasar, BPTU HPT Pelaihari, BPTU HPT Sembawa, BBPTU HPT Baturraden and CV Cahaya Baru Semarang selected as the observed locations. Environmental data pertaining to elevation (m a.s.l), annual rainfall (mm/year), temperature (C), humidity (%), and light intensity (Lumen hour) were collected for each unit. Seed production parameters were seed yield (g) and seed weight (g per 1,000 seeds). Seed quality parameters encompassed water content (%), purity (%) and viability (%). The analysis in this study involved assessing the mean differences in these parameters through the application of Analysis of Variance (ANOVA). The result showed the seed production and quality were divorce across SPUs, with BPTU HPT Sembawa and CV Cahaya Baru Semarang demonstrating outstanding seed production, while BPTU HPT Pelaihari maintains high quality standards. The highest positive correlation emerged between elevation and water content (r=0.72), highlighting the influence of altitude on the moisture content of seeds. The strongest negative correlation was observed between temperature and water content (r= neg. 0.76), emphasizing the role of temperature in drying seeds. Keywords: Indigofera, seed production, seed quality, microclimatic conditions Share Link | Plain Format | Corresponding Author (Elly Dianita Sari)

11	Animal Nutrition	ABS-18
Productivity of Elephant Grass Pakchong (Pennisetum purpureum cv Pakchong) and Elephant Grass Taiwan (Pennisetum purpureum cv Taiwan) Cultivated Based on Different Stem Cutting Sizes A, Fanindi, E. Sutedi, Sajimin, I. Herdiawan, Harmini, Baehaki, Karya, A. Setiawan, I. Solihat, A. Mulyadi, F.A. Pamungkas Research Center for Animal Husbandry, National Research and Innovation Agency Abstract In Indonesia, elephant grass seeds used by farmers are in the form of cuttings or vegetative material. Currently, there is no standard for the cuttings used to produce high-productivity elephant grass. Therefore, research was conducted to determine the optimal size of cuttings for achieving good elephant grass productivity. The study was conducted in the field station area of Rancamaya, Bogor. A factorial group randomized design with 5 replications was employed for the research. The first factor was the type of elephant grass, consisting of 1) Pakchong and 2) Taiwan, while the second factor was the size of the cuttings, consisting of 1) 5 cm long cuttings, one node, 2) 10 cm long cuttings, one node, 3) 15 cm long cuttings, one node, and 4) control (two nodes as per usual farmer practice). The research results indicated that there was an interaction between the type of elephant grass and the size of cuttings for leaf length, fresh and dry leaf weight, dry stem weight, and total dry weight. The 5 cm cuttings of Pakchong had the highest fresh and dry leaf weights (P<0.05). The highest dry stem and total weight were obtained with the control size cuttings of Pakchong. The number of tillers was influenced by the size of cuttings, with the control size cuttings having the highest number of tillers, although not significantly different from the number of tillers for the 5 cm and 15 cm cuttings. The highest total fresh weight was obtained with the 5 cm cuttings and Pakchong. This study indicates that 5 cm cuttings can be used as cutting material to produce Pakchong and Taiwan with optimal forage production. Further research is needed to assess the forage quality. Keywords: Seed, forage, vegetative, production Share Link | Plain Format | Corresponding Author (Achmad Fanindi)

12	Animal Nutrition	ABS-20
The Potential of Frozen Rumen Fluid with Dimethyl Sulfoxide And Glucose As Cryoprotectant on In Vitro Gas Production And Digestibility Sayyid Muhammad Djafar(a*), Divia Sekar Ayu Putri Darmawan(a), Adam Suryapratama(a), Hendrawan Soetanto(a) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *sayyid.muhammad.djafar[at]gmail.com Abstract As the pressure from the animal welfare community increases there is an urgent need to find the alternative rumen fluid (RF) source as an essential inoculum for in vitro digestibility studies. The objective of this study is to support the previous studies utilizing fresh RF from abattoir with and without cryoprotectants during the freezing process. A 2x3x3 factorial design was used to ascertain the effectiveness of dimethyl sulfoxide (DMSO) and glucose as cryoprotectants to frozen RF varying from 4, 11, and 18 days to measure the in vitro gas production (IVGP) and digestibility of a complete feed consisted of elephant grass and commercial concentrate at 1 : 1 ratio. factor A being the preservation methods (A: immediate & B: gradual freezing), factor B being the cryoprotectants (P0: None | P1: 1% DMSO | P2: 1% DMSO + 0.8% Glucose), and factor C being the preservation time (4, 11, and 18 days). Experimented on in vitro gas production (IVGP) and digestibility (IVD), the analysis carried out mixed results. IVGP shows that the use of DMSO and glucose as cryoprotectants (P1A, P1B, P2A, & P2B) only held back quality performance and made the non-cryoprotected frozen RF (P0A) is the most applicable method for preservation that lasts until 18 days. IVD however resulted the most similar frozen RF treatment with gradual freezing that use DMSO as cryoprotectant (P1B). Thus making DMSO and glucose influenced nothing significant to the frozen RF. Maintaining the reduction of animal welfare while simultaneously keeping the data variation low is achievable and efficient. Keywords: Cryoprotectant, DMSO, Glucose, In Vitro, Rumen Fluid Share Link | Plain Format | Corresponding Author (Sayyid Muhammad Djafar)

13	Animal Nutrition	ABS-21
Effects of Dimethyl Sulfoxide As Cryoprotectant For Rumen Fluid On In Vitro Gas Production And Volatile Fatty Acid Content Sayyid Muhammad Djafar(a*), Alfina Salsabila(a), Aksauri Hana Malis(a), Hendrawan Soetanto(a) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *sayyid.muhammad.djafar[at]gmail.com Abstract Initiating the research hypothesis of dimethyl sulfoxide(DMSO) potential for preserving the rumen fluid(RF), this experiment^s urgency is based on welfare that seems violated when fistulated cattle used regulary for the inoculum source. The alternative was experimented by taking loads of rumen fluid from Madura cattle from slaughterhouse, to prevent welfare violations by rumen fluid extraction, and to reduce data variation since only single inoculum source used. The in vitro gas production enrolled by 2x2x3 factorial design, with a couple freezing treatments in -21 C using 5% DMSO and unvarnished as the factor A, which complemented by standard and complete feed each as the factor B. They were preserved for 2, 5, and 7 days as the factor C. Post-analysis results show that preservation time on unvarnished differed significantly with total gas produced (P>0.01), and did not impacted on the potential (P<0.05) and the rate (P<0.05) of gas production. On the other hand, specific volatile fatty acid content of the treated RF shows that DMSO impacted significantly on butyric acid (P>0.01), differed on propionic acid (P>0.05), and did not alter anything on acetic acid (P<0.05). By having more volatile fatty acid on the DMSO RF, the potential gas produced is not as high as the unvarnished, thus making the addition of DMSO to the RF is not benefited the preservation yet. On the flipside the potency of unvarnished RF to be preserved is applicable for reusability. Keywords: DMSO, In Vitro Gas Production, Rumen Fluid, Volatile Fatty Acid Share Link | Plain Format | Corresponding Author (Sayyid Muhammad Djafar)

14	Animal Nutrition	ABS-27
The Effect of the Addition Herbal Dregs Fermentation as a Feed Additive on the Internal Quality Eggs of Quail (Coturnix coturnix japonica) Prihatiningsih (a*) and Osfar Sjofjan (b) a) Bachelor Student, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *prihatiningsih[at]student.ub.ac.id b) Lecture, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract The aims of this study was to determine the effect of adding herbal dregs as a feed additive to basal feed on the internal quality of quail eggs. This study used 200 quails of the Blaster strain aged 30 days, by placing 10 quail populations in each flock cage. The method used in this study was an experiment design using 5 treatments and 4 replicates. The treatment consisted of T0 = control treatment, T1 = 0.25% herbal dregs, T2 = 0.50% herbal dregs, T3 = 0.75% herbal dregs, and T4 = 0.1% herbal dregs. The variables observed is included haugh unit, egg yolk volume, egg white volume, and egg yolk colour. The data obtained were statistically analyzed using the Analysis of Variance (ANOVA) with a Complete Randomized Design (CRD), followed by the Duncan Multiple Distance Test (DMRT) if there were significant differences. The results showed that the addition of herbal dregs to basal feed had no significant effect (P>0.05) on egg white volume, yolk volume, and haugh unit. On the other hand, it had significant effect (P<0.05) on egg yolk colour. The conclusion of this study is that the addition of fermented herbal dregs can improve the internal quality of quail eggs, especially the colour of the yolk with the best herbal dregs fermentation treatment at 0.1%. Keywords: Herbal Dregs, Internal Quality of Eggs Quail, Quail Share Link | Plain Format | Corresponding Author (Prihatiningsih Prihatiningsih)

15	Animal Nutrition	ABS-28
Effect of the combination phytobiotic and probiotic on external quality of egg laying ducks Yuli Frita Nuningtyas (a*), Muhammad Halim Natsir (a), Eko Widodo (a), Heli Tistiana (a), and Osfar Sjofjan(a) Faculty of Animal Science, Universitas Brawijaya Abstract This study aims to evaluate the use of a combination of phytobiotics and probiotics on external egg quality in laying ducks. The number of ducks used in this study was 120 which were divided into 4 treatments and 5 replications, each replication consisting of 5 ducks. The treatments were T1: control, T2: 0.25% combination, T2: 0.5% combination and T3: 0.75% combination. The external egg quality variables observed were egg weight, egg length, egg width and egg index. Observations on variables were carried out once a week during four weeks,every repetation using 2 eggs. The data obtained were analyzed statistically using a completely randomized design ANOVA. If the results of the show significantly different then continue with the DMRT test. Based on the results, it shows that the different levels of the combination phytobiotics and probiotics showed significantly different results (p<0.05) on egg weight in the 1st and 4th weeks, egg length in the 1st week, egg width in the 4th week and egg index in the 1st week. week 1. The addition of 0.5% combination of phytobiotics and probiotics can improve the external quality of duck eggs. Keywords: Laying ducks, external, egg quality, phytobiotic,probiotic Share Link | Plain Format | Corresponding Author (Yuli Frita Nuningtyas)

16	Animal Nutrition	ABS-30
Effect Of Feeding A Combination Of Probiotic Feed Additives, Phytobiotics, And Microencapsulated Acidifiers On Broiler Villi Characteristics Andhani Kurnia Rahman, Irma Widi Astuti, Aurel Vinda Syahrira, Ardian Yhosi Setyawan, Firmansyah Budi Pratama dan Irfan H. Djunaidi Brawijaya University Abstract The ban on the use of Antibiotic Growth Promoter (AGP) in feed makes farmers doubt the efficiency of feed conversion by poultry, especially broilers. This study aims to determine the effectiveness of the addition of natural feed additives on the production performance and quality of broilers. The sample used in this study was DOC (Day Old Chicken) Cobb strain with the trademark CP 707 as many as 75 heads. The method used was experimental designed using a completely randomized design with three treatments and five replicates used five broilers per replicate. The treatments were T0 = basal feed, T1 = basal feed with 0.5% natural feed additive, and T2 = basal feed with 1.0% natural feed additive. The variables used in this study were villous characteristics including villous length, number of villi, and depth of villous crypts. Data were analyzed with ANOVA and continued with Duncan^s multiple range test. The results showed that the test of villi characteristics using natural feed additive combination of probiotics-phytobiotics-acidifier microencapsulation gave a significantly different effect (P>0.05) on the number and depth of villi crypts, but gave a significantly different effect (P>0.01) on villi length. The use of natural feed additive combination of probiotics-phytobiotics-acidifiers gives effective results on the characteristics of intestinal villi in the ileum section giving good results at the level of use of 1.0% and can be used to replace antibiotics. Keywords: natural feed additives, broilers, microencapsulation, characteristics of intestinal villi Share Link | Plain Format | Corresponding Author (Andhani Kurnia Rahman)

17	Animal Nutrition	ABS-44
The effectiveness of using a combination of saponin and vaccine given in ovo on hatchability and early growth performance of local chickens Rantan Krisnan (a*), Cecep Hidayat (a), Astu Unadi (a), Isbandi (a), Rusdiana Supardi (a), Simon P. Ginting (a), Bachtar Bakrie (a), Chalid Talib (a), Andi Tarigan (a), Mohammad Ikhsan Shiddieqy (a), Muhamad Hamdan (a), Andi Suhandi (a), Winwin Widaringsih (a) a*) Research Organization for Agriculture and Food, National Research and Innovation Agency a) Research Organization for Agriculture and Food, National Research and Innovation Agency Abstract Providing nutrients as early as possible (in ovo feeding) to local chickens is an important breakthrough for the development of poultry businesses in Indonesia, because it will be a big opportunity to produce superior local chicks (day old chick) that has been enriched with important nutrients before hatching. A study has been carried out to determine the correct dosage for using a combination of saponin and vaccine in an in ovo formula on hatchability and early growth performance. There were four levels of saponin use (0.0- 1.5- 3.0- 4.5 mikrogram) arranged using a factorial design with two factors, namely saponin level as the first factor, while whether or not vaccine was used as the second factor, so there were eight formula combinations. Each formula combination was dissolved in phosphate buffered saline, then injected into the amnion of fertile eggs of Sentul chickens during incubation for 18 days (0.5 ml/egg) using an automatic injector machine with a 20G needle (diameter 0.9 mm and length 8 mm). The results showed that the hatchability of Sentul chickens ranges from 66 until 93%. The combination of vaccinated formula containing 0.0 mikrogram saponin had the highest hatchability value (93.33%) and the best body weight gain and feed conversion. This formula combination was significantly different (P<0.05) from other formula combinations. A trend was seen that increasing the dose of saponin used showed a decrease in the performance of early growing chickens. This happens in combination formulas with vaccines or without vaccines Keywords: in ovo, saponin, vaccines, hatchability, early growth, local chicken Share Link | Plain Format | Corresponding Author (Rantan Krisnan Krisnan)

18	Animal Nutrition	ABS-46
AGRONOMIC CHARACTERISTICS OF RED NAPIER GRASS (Pennisetum purpureum cv. RED) UNDER DIFFERENT LEVEL OF NITROGEN FERTILIZATION AND HARVEST AGES Novaliana Rukmana, Rizka Muizzu Aprilia, Kusmartono, and Ifar Subagiyo Universitas Brawijaya Abstract This experiment aimed to find out the morphological characteristics and biomass DM production of Red Napier grass (Pennisetum purpureum cv. Red) under different levels of nitrogen fertilization and harvest ages. The grass stem cutting were planted on polybags. A Completely Randomized Factorial Design (CRFD) was employed comprised of 2 treatment factors. The first factor was harvest age of K1: 50 days of age K2: 60 days of age and K3: 70 days of age and the second factor was level of N fertilization comprised P1: no fertilization, P2: 100 kg N /ha/year, P3: 200 kg N/ha/year, P4: 300 kg N/ha/year. The treatments were applied after the grasses were trimmed at 90 days after planting. The variables measured include plant height, number of tillers, leaves-to-stem ratio, and biomass production. Data collected were subjected to analysis of variance and least significant difference test. It was found that interaction of treatments significantly (P<0.01) affects plant height and biomass DM production. Treatments of age of harvest and level of N fertilization, respectively significantly (P<0.01) affect either number of tillers or leaves-to-stem ratio. It was concluded that K3 and P3 gave the highest value for all variables measured. Keywords: Red Napier, plant height, tillers, stem, leaves, dry matter Share Link | Plain Format | Corresponding Author (Novaliana Rukmana)

19	Animal Nutrition	ABS-59
Evaluation of Lamtoro Leaf (Leucaena Leucocephala (Lam.) De Witt.) Supplementation in Feed on Nutritive Value and Digestibility in Lactation Period Peranakan Ettawa Goats in Vivo Hartutik (a,*), Marjuki (a), Asri Nurul Huda (a), Galuh Yolanda Fitri (b), Devan Puji Dewantara (b), Yuli Nur Latifah (b), Indah Kartikasari (b), dan M. Alif Fayqol Azmi (b) a) Lecturer of Faculty Animal Science, Brawijaya University, Malang 65145, Indonesia b) Student of Faculty Animal Science, Brawijaya University, Malang 65145, Indonesia *) hartutik[at]ub.ac.id Abstract This study evaluated the effect of Leucaena leves (Laucaena laucochepala) supplementation on the digestibility of Ettawa crossbreed goats. Twelve Ettawa crossbreed goats during lactation period divided into 6 groups were allocated in a group randomized design with two treatments and six replicates (two goats per replicate). Treatments consisted of control (T0) in the form of 100% Leucaena leaves and (T1) 50% Leucaena and 50% concentrate. This study began with the preliminary stage, adaptation stage, data collection and analysis. Samples of feed given, the amount of feed given, the amount of leftover feed given and feces samples from each individual goat were recorded every day for data collection. Data from this study were tabulated using Microsoft Excel software program, then analyzed using T test analysis and Randomized Group Design. The results showed that the CFD (Crude fiber digestibility) value had a significantly different effect (P<0.05), Leucaena leaves high Crude Protein content can affect digestibility to be higher than T1 which is composed of 50% Leucaena + 50% concentrate. ADF digestibility value showed no significant difference (P>0.05). NDF digestibility showed no significant difference (P>0.05). Hemicellulose digestibility showed no significant difference (P>0.05). The digestibility of organic matter showed no significant difference (P<0.05). Dry matter digestibility showed significantly different effect (P<0.05). The digestibility value of crude protein showed a significantly different effect (P<0.05). The results of the study can be concluded that Leucaena leaves supplementation can increase the digestibility value. Keywords: Keywords Digestibility, Ettawa crossbreed goats, Leucaena laucochepala, suplementation Share Link | Plain Format | Corresponding Author (Hartutik Hartutik)

20	Animal Nutrition	ABS-60
Response of goat fed on Indigofera Sp. and Pennisetum purpureum cv. Mott Charles L. Kaunang*, Agustinus Lomboan*, Endang Pudjihastuti* Faculty of Animal Science, Sam Ratulangi University Manado Abstract This study aimed to evaluate the response of goat fed on Indigofera Sp. and Pennisetum purpureum cv. Mott. We randomly assigned goats into three treatments and five replications using Completely Randomized Design (CRD). The treatments were T0= 0% Indigofera sp. + 100% Pennisetum purpureum cv. Mott T1 = 20% Indigofera sp. + 80% Pennisetum purpureum cv. Mott, T2= 40% Indigofera sp. + 60% Pennisetum purpureum cv. Mott. All data was analyzed with analysis of variance (ANOVA) test and Duncan multiple range test. We assessed daily weight gain, dry matter intake, digestibility of dry matter, and feed efficiency as study outcomes. Feeding of Indigofera sp. and Pennisetum purpureum cv Mott in different doses significantly affected (p<0.05) dry matter digestibility, dry matter intake, daily weight gain and feed efficiency. In all variables, treatment T2 significantly affected (p<0.05) all study outcomes compared to T1 and T0. Mean dry matter digestibility ranged from 41.5 to 65.0. Mean dry matter intake ranged from 260.2 to 466.6 g/head/day. Mean daily weight gain ranged from 22.30 to 72.4 g/head/day. Mean feed efficiency in this study ranged from 22.30 to 72.4 g/head/day. We found an optimal response of goat after feeding with 60% Pennisteum purpureum cv. Mott + 40% Indigofera sp. Keywords: Indigofera sp., Pennisetum purpureum cv. Mott, daily weight gain, feed efficiency, dry matter intake, dry matter digestibility Share Link | Plain Format | Corresponding Author (Charles Lodewijk Kaunang)

21	Animal Nutrition	ABS-61
Performance of goat fed on basal feed and Indigofera zollingeriana Endang Pudjihastuti*, Agustinus Lomboan*, Charles L. Kaunang* *Faculty of Animal Science, Sam Ratulangi University Manado Abstract This study aimed to measure the potential and basal feed introduction (corn stover, gamal leaves, Panicum maximum) with the addition of Indigofera zollingeriana, which is expected to increase the goat performance. Fifteen goats aged 9-12 months were randomly assigned in completely randomized design (CRD) to one of three treatments and five replications. The treatment were P1 = 60% basal feed + 40% Indigofera zollingeriana, P2 = 70% basal feed + 30% Indigofera zollingeriana, P3= 80% basal feed + 20% Indigofera zollingeriana. We assessed the study outcomes as follows: daily weight gain (DWG), dry matter intake (DMI), feed efficiency and physiological responses. Data was statistically analyzed with Analysis of Variance (ANOVA) and Duncan^s multiple range test. ANOVA test showed that the treatments affected significantly (p<0.05) the DMI, DWG, feed efficiency and respiratory rate. Based on multiple range test, treatment P1 was significantly different (p<0.05) than treatment P2 and P3. Mean DMI ranged from 265.8 to 380.32 g/head/day, mean DWG ranged from 33.28 to 65.62 g/head/day, mean feed efficiency ranged from 0.12 to 0.17%, mean respiratory rate ranged from 22 to 32 rate per minute. Statistical analysis showed that the treatment did not significantly (p>0.05) affect rectal temperature and heart rate. Mean rectal temperature and heart rate were 38.2-39.8&#8304-C and 80-90 beat per minute, respectively. In conclusion, the administration of 60% basal feed (20% corn straw, 20% gamal leaves and 20% Panicum maximum) and 40% Indigofera zollingeriana could improve the goat performance. Keywords: goat performance, basal feed, Indigofera zollingeriana, daily weight gain, dry matter intake, feeding efficiency, physiological response Share Link | Plain Format | Corresponding Author (Endang Pudjihastuti)

22	Animal Nutrition	ABS-63
Effect of Nutmeg Meal on microbial abundance of biohydrogenation process, and fatty acid profile of goat rumen fluid in vitro R.A. Pratiwi, Muhlisin, A. Astuti, C. Hanim and A. Kurniawati Faculty of Animal Science, Faculty of Animal Science, Gadjah Mada University Jl. Fauna 3 Yogyakarta 55281-Indonesia, Laboratory of Nutrition Biochemistry *author muhlisin.fapet[at]ugm.ac.id Abstract This study aimed to evaluate the effect of nutmeg meal as a source of phenol on the abundance of main microbes in the biohydrogenation process and the fatty acid profile of goat rumen fluid in vitro. This study used a randomized complete block design with four treatments and three replicates of in vitro batches, each replicate in duplicate. Treatment feed ingredients consisted of dwarf elephant grass, soybean meal, wheat pollard, canola oil and nutmeg meal with the composition of P0 (40:10:45:5:0)- P1 (40:10:40:5:5)- P2 (40:10:35:5:15)- P3 (40:10:30:5:10). Fermentation using the in vitro gas production method described by Menke and Steinggas with an incubation period of 48 hours. Parameters observed included microbial abundance and fatty acid profile of goat rumen fluid. The statistical analysis showed that adding nutmeg meal starting at the 5% level decreased the abundance of the main biohydrogenation process bacteria anaerovibrio, butyrivibrio fibrisolvens, and pseudobutyrivibrio. Total saturated fatty acid and stearate fatty acid concentrations decreased (P<0.05), while total unsaturated fatty acid, linoleic and oleic acid concentrations increased (P<0.05). Keywords: phenol, microbial abundance, biohydrogenation, rumen fermentation, fatty acid profile, in vitro Share Link | Plain Format | Corresponding Author (Renna Ambar Pratiwi)

23	Animal Nutrition	ABS-67
The Effect of Using Turmeric, Ginger, and Teak Leaf Extract Nanoparticles as Feed Additives on the Total Plate Count of Lactic Acid Bacteria, E. Coli, and Salmonella sp. Donny Tri Ardiansyah (a), Yuli Frita Nuningtyas (b), and Muhammad Halim Natsir (b) * a) Master Student Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia b) Lecture Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *Corresponding author: emhanatsir[at]ub.ac.id Abstract This study evaluated the effect of using ginger, turmeric and teak leaf extracts in the form of nanoparticles on the total plate count of LAB, E. Coli and Sallmonela Sp. in broiler chicken digesta. 200 broiler chickens were divided into 5 groups allocated in a randomized block design with 5 treatments and 4 replications (10 broiler chickens per replication). Treatment consisted of control (P0) in the form of 100% commercial feed, (P1) in the form of the addition of 0.2% extract, (P2) in the form of the addition of 0.4% extract, (P3) in the form of the addition of 0.6% extract, and (P4) in the form of adding 0.8% extract. This research began with the extract making stage, nanoparticle synthesis stage, data collection and analysis. Samples of feed given, the amount of feed given, the remaining amount of feed given from each animal replication were recorded every day for data collection. Data from this study were tabulated using the Microsoft Excel software program. Data were subjected to ANOVA and followed by Duncan Multiple Range Test at 5% level. The results of the study showed that giving feed additives from ginger, turmeric and teak leaf extracts in the form of nanoparticles had a significant effect (P<0.05) on increasing the LAB population, reducing the population of E. coli and salmonella. In conclusion, giving feed additives from ginger, turmeric and teak leaf extracts in the form of nanoparticles up to 0.8% increased the population of lactic acid bacteria, reducing Escherichia coli in Sallmonela. Keywords: Extract, Nano Particles, Feed Additive, TPC, E. Coli, Salmonella, LAB Share Link | Plain Format | Corresponding Author (Muhammad Halim Natsir)

24	Animal Nutrition	ABS-76
Improving the productivity of Joper chickens with fermented soy milk waste (SMW) Nining Haryuni1 , Yuli Arif Tribudi2, Niswatin Hasanah3, and Ragil Angga Prastya4 1Department of Animal Husbandry, Nahdlatul Ulama Blitar University, Blitar City 66117, East Java, Indonesia 2Department of Animal Science, Faculty of Agriculture, Tanjungpura University, Pontianak, West Kalimantan, Indonesia 3Department of Animal Husbandry, Jember State Polytechnic, Jember 68101, East Java, Indonesia 4Department of Veterinary Medicine, School of Health and Life Sciences (SIKIA), Universitas Airlangga, Surabaya 60115, Indonesia Abstract The productivity of Joper chickens can be increased by improving feed quality using fermented soy milk waste (SMW). This research was carried out through biological research using 100 day old chicks (DOC) Joper in healthy conditions using a completely randomized design (RAL). The research was carried out using 5 treatments of fermented SMW levels in feed (0, 5, 10, 15 and 20%) and each treatment used 5 DOC Joper and was repeated 4 times. SMW in this study was fermented using effective microorganism 4 (EM-4) at a dose of 7.50% of the weight of SMW. Statistical analysis showed that the fermented SMW level was very significant (p<0.01) increased feed intake, body weight gain and body weight of Joper chickens and reduced mortality and FCR. The average feed intake ranged from 2071.50-2207.00 g/head- body weight gain (14.71-25.55 g/bird/day)- body weight (556.00-934.25 g/bird)- mortality (0.84-1.25%) and FCR (2.34-3.75). This research can be concluded that improving feed using fermented SMW can increase the productivity of Joper chickens. The best productivity of Joper chickens was using 15% SMW. Keywords: Productivity, fermentated, soy milk waste, chicken Share Link | Plain Format | Corresponding Author (Yuli Arif Tribudi)

25	Animal Nutrition	ABS-78
OPTIMIZING PERFORMANCE OF MADURA CATTLE IN SMALLHOLDER FARMS BANGKALAN MADURA Eva Icahyaningrum, Kusmartono, Mashudi, and Poespitasari Hazanah Ndaru Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, East Java, Indonesia Abstract Bangkalan Regency is located on Madura Island, which is one of the districts that has a population of 1,047,783 Madura cattle or around 21.7% and 5.99% of the national cattle population. Madura cattle are evenly distributed on Madura Island. Forages that are often used as feed are field grass and agricultural waste. In Vivo research uses concentrate to support the growth of beef cattle in Bangkalan Regency. The concentrate feed ingredients used consist of cassava waste, palm kernel cake, copra meal and tofu waste. The treatment given to Madura cattle consisted of four treatments.The feed treatments consisted of T1 (cassava waste 40% + copra meal 28% + palm kernel cake 30% + tofu waste 2%), T2 (cassava waste 42% + copra meal 36% + palm kernel cake 19% + tofu waste 3%), T3 (cassava waste 38% + copra meal 31% + palm kernel cake 27% + tofu waste 4%), and T4 (cassava waste 41% + copra meal 33% + palm kernel cake 21% + tofu waste 5%). Data on nutrients content and consumption nutrition were analyzed using a randomized block design (RBD) .The results show that local feed ingredients in bangkalan district can influence nutrion content and comsumption nutrition. Keywords: Keywords: local feed, madura cattle, nutrient content, consumption nutrition Share Link | Plain Format | Corresponding Author (Eva Icahyaningrum)

26	Animal Nutrition	ABS-79
DIGESTIBILITY VALUES OF MADURA CATLLE FEED IN BANGKALAN REGENCY Eva Icahyaningrum1, Kusmartono1*, Mashudi1, Poespitasari Hazanah Ndaru1, and Aprilia Dwi Kartika1 1Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *Corresponding author: kusmartono_ansi[at]ub.ac.id Abstract The problem that often occurs in the development of beef cattle on smallholder is the low level of livestock productivity, this is because the rearing system implemented is still conventional so that feed requirements have not been met, both in quality and quantity. The study was conducted to determine the values of nutrient content, dry matter digestibility (DMD), organic matter digestibility (OMD), concentration NH3 and efficiency microbial protein synthesis (EMPS) value of beef cattle feed in Bangkalan Regency. This study used an in vitro method assigned in a randomized block design (RBD) with six treatments and three replication. The following three treatments based on feed survey in Bangkalan Regency (A1, A2, A3) and three treatment based on feed scenarios (B1, B2, B3) repectively, feed for Madura heifer, Madura cattle and Limousine crossbred. The results showed that the feed from the survey was of lower quality compared to the scenario feed, especially its protein content. Scenario feed can improved DMD and OMD value and this was closely related to improvements of rumen NH3 concentration with also contributed to EMPS value improvement. Keywords: Madura cattle, Limousine crossbred, digestibility, NH3 concentration, EMPS value Share Link | Plain Format | Corresponding Author (Eva Icahyaningrum)

27	Animal Product Technology	ABS-2
Polycyclic Aromatic Hydrocarbons (PAH) Analysis of Commercial Goat Sate In All Cities East Java Gadis Apriani (a), Lilik Eka Radiati (b), Premy Puspitawati Rahayu (b), Eko Saputra (c), Djalal Rosyidi (b*) a) Student of Animal Product Technology, Faculty of Animal Science, University Brawijaya, Malang b) Lecturer of Animal Product Technology, Faculty of Animal Science, University Brawijaya, Malang c) BPPSDMP-Batu, Indonesia Ministry of Agriculture Abstract Satay is one of Indonesia^s delicious national foods besides rendang, so-to, meatballs, and fried rice but there is still much research to be done. Heating meat is an important process for nutritional quality and bioavailability, extending shelf life, obtaining better sensory and functional properties, releasing bioactive components, producing benefical compounds, destroying anti-nutrive subtance, and activating disease pathogens. Processing of satay at high temperaturs will contain carnogenic mutagen Policyclic Aromatic Hydrocarbons (PAH). The process of roasting goat satay turns out to produce carcinogens, Heterocyclic Aromatic Amine (HAA) and Polycyclic Aromatic Hydrocarbone (PAH) as well as other side products that are potentially harmful to our health in the long term. The long-term goal of this research is to produce goat satay which is free of PAH carcinogens which is healthier and safer for the community. The specific target of this research is to investigate the formation of Polycyclic Aromatic Hydrocarbone (PAH) carcinogens in commercial goat satay sold in all cities throughout East Java. Research is non-experimental with surveys. The survey was conducted at various commercial goat satay stalls located throughout East Java. Sampling was carried out 3 place. The parameters of this research include: (1) levels of 4 types of PAH compounds (PAH4), namely: benzo(a)pyrene (BaP), benzo(a)antharacene (BaA), benzo(b)floranthene (BbF) and chrysene (Chr)- (2) identification of complementary vegetables for satay dishes- (3) the weight of a portion (10 skewers) of goat satay. The samples used in this study were 27 types of chicken satay (SK1-SK27). The results of this study indicated that SK12 was detected to contain chrysene-type Policyclic Aromatic Hydrocarbons (PAH) the highest scale 1.545 mg/kg. Policyclic Aromatic Hydrocarbons (PAH) oc-curs due to the influence of the type of meat, temperature, and the type of combus-tion used. Keywords: Benzo(a)pyrene, Goat Satay, Polycyclic Aromatic Hydrocarbone Share Link | Plain Format | Corresponding Author (GADIS APRIANI)

28	Animal Product Technology	ABS-6
THE EFFECT OF EGGSHELL DIPPING WITH VARIOUS TYPES OF OILS AND STORAGE DURATION ON THE WEIGHT LOSS AND SHELL THICKNESS OF TABLE EGGS Fahmi Axel Revanda (a), Oktavia Rahayu Puspitarini (b*) Animal Husbandry Study Program, Faculty of Animal Husbandry, University of Islam Malang Correspondent E-mail: oktaviarahayu[at]unisma.ac.id Abstract This study aims to analyze the effect of dipping eggs in various types of oils and the duration of storage on weight loss and shell thickness of table eggs. The research material includes 45 chicken eggs, pure coconut oil, palm oil, corn oil, a screw micrometer, and a scale. The method used was experimental, with factorial completely randomized design in a 3x5x3 factorial pattern. Treatment A consists of oil types (A1: pure coconut oil, A2: palm oil, A3: corn oil), and treatment B: the storage duration of eggs at room temperature (B1: 25, B2: 30, B3: 35, B4: 40, and B5: 45 days). The observed variables were the egg weight loss and shell thickness. Data were analyzed using analysis of variance and further analyzed using the Duncan Multiple Range Test (DMRT), utilizing SPSS version 16.0. The results show that the storage duration at room temperature was significantly affects (P<0.05) on th egg weight loss and shell thickness. The type of oil was significantly affects (P<0.05) shell thickness. The interaction between the type of oil and storage duration did not had a significantly affect (P>0.05) egg weight loss and shell thickness. In conclusion, the best treatment for dipping eggs is using pure coconut oil and storing them at room temperature for up to 40 days. Keywords: Egg dipping- Type of oil- Storage time- Room temperature- Weight loss- Shell thickness Share Link | Plain Format | Corresponding Author (Fahmi Axel Revanda)

29	Animal Product Technology	ABS-13
Propolis Unveiled: Exploring its Potential as a COVID-19 Therapeutic Agent through Network Pharmacology and Molecular Docking Khalish Arsy Al Khairy Siregar(a,b), Paula Mariana Kustiawan(a*), Anissa Nofita Sari(b,c), Feri Eko Hermanto(b,c) a) Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Borneo 75124, Indonesia *pmk195[at]umkt.ac.id b) Bioinformatics Research Center, Indonesian Institute of Bioinformatics (INBIO), Malang, Indonesia c) Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, 53226 USA Abstract This study investigates the potential of propolis, a natural substance, as a COVID-19 treatment. Amid the ongoing pandemic challenges, there^s a growing need for complementary or alternative treatments to combat the virus. While propolis has previously shown antiviral effects against COVID-19, its specific workings in the human body have remained unexplored. To unveil this mechanism, the study employed network pharmacology, molecular docking, and molecular dynamics simulations. The study identified propolis^s target proteins and active components using various databases, and it also mined COVID-19-related genes. Analysis of KEGG pathways and GO enrichment revealed associations with cancer, COVID-19, and AGE-RAGE. A protein interaction network was constructed, highlighting key targets like MAPK1. Docking simulations found that Broussoflavonol F, Glyasperin A, and Sulabiroins had the best binding affinity, surpassing the native ligand, confirming their potential as COVID-19 treatment candidates. Molecular dynamic simulations showed stable behavior of the complexes. In summary, this study suggests that propolis could be a promising COVID-19 treatment, primarily by inhibiting the MAPK1 pathway. However, further research is required to validate these results and develop practical applications to mitigate COVID-19^s impact. This study underscores the importance of network pharmacology and computational models in understanding natural compounds like propolis in the human body, offering potential avenues for future research and treatment strategies. Keywords: COVID-19- MAPK1- Molecular Docking- Network Pharmacology- Propolis Share Link | Plain Format | Corresponding Author (Khalish Arsy Al Khairy Siregar)

30	Animal Product Technology	ABS-14
Effect Of Adding Butterfly Pea Extract (Clitoria Ternatea L.) The Quality Of Frozen Yogurt In Terms Of Organoleptic, L*a*b* Colour, Colour Spectrum, And Optical Microscope Jamilah Dinna Saputeri(a) Manik Eirry Sawitri(b) Ria Dewi Andriani(c) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia dinnasaputeri26[at]student.ub.ac.id b) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia c) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract Keywords: Antosianin, Chroma, Hue Angel, Sensory Properties. Share Link | Plain Format | Corresponding Author (Jamilah Dinna Saputeri)

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