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61	Animal Production	ABS-72
Early Growth Assessment of F2 Indonesian Chicken Crosses: A Performance Study Caesa Rizq Firdaus(a), Yesita Vera Saraswati(b), Dyah Maharani(b) , Yuny Erwanto(c), Budi Guntoro(d), Michael Haryadi(e), and Heru Sasongko(a*)) (a)Department of Animal Production, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia *herusasongko[at]ugm.ac.id (b)Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia (c)Department of Animal Products Technology, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia (d)Department of Livestock Socio-Economics, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, Indonesia (e)Department of Microbiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia Abstract Body weight and feed conversion ratio (FCR) are crucial factors for assessing production potential in local chickens. This study utilized a total of 116 F2 crossbred chickens resulting from the mating of Indonesian local chicken breeds, including Kampung Unggul Balitbangtan, Merawang Bangka, and Merawang Sembawa. The chickens were divided into six crossbred groups: BS1 (n = 19), BS2 (n = 19), BS3 (n = 20), BS4 (n = 19), BS5 (n = 20), and BS6 (n = 19), all managed under uniform conditions. Measurements of body weight and feed consumption during the pre-starter phase were taken at weeks 0, 1, 2, and 3 using a digital scale. Data from this study were subjected to ANOVA analysis, followed by a Duncan multiple range test. The results revealed significant differences in body weight and FCR among all BS chicken groups (P < 0.05). Notably, BS3 exhibited the highest performance in both body weight and FCR analyses, achieving 36.92 g at week 0, 240.45 g at week 3, and an overall FCR of 1.97. Keywords: body weight, crossing, FCR, Indonesian local chicken Share Link | Plain Format | Corresponding Author (Caesa Rizq Firdaus)

62	Animal Production	ABS-77
Effects of Egg Storage Duration on the Intestinal Length of Arab Chickens at Two Weeks of Age Faizal Andri*, Filoza Marwi, Eka Nurwahyuni, Dyah Lestari Yulianti, Heni Setyo Prayogi, and Edhy Sudjarwo Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *Corresponding author: f.andri[at]ub.ac.id Abstract This study evaluated the effects of egg storage duration on the intestinal length of Arab Chickens at two weeks of age. The treatments consisted of different egg storage durations, specifically 1 day, 4 days, 7 days, and 10 days. The hatching eggs underwent an incubation period of 21.5 days. Following hatching, 25 one-day-old Arab Chickens were selected from each treatment and placed into 5 separate replicate pens (with five chicks in each replicate). These chicks were then raised for two weeks. The observed variables included the duodenum, jejunum, ileum, caeca, colon, and the total intestinal length. Data analysis was carried out using a one-way analysis of variance, followed by a post-hoc Duncan test. The results indicated that the egg storage duration did not have a substantial effect (P>0.05) on the jejunum, caeca, and colon lengths of Arab Chickens. However, egg storage for 7 and 10 days significantly reduced (P<0.05) ileum and total intestinal length. Furthermore, 10 days of storage also notably reduced (P<0.05) duodenum length. To sum up, prolonged egg storage for 7 days or more appears to negatively affect the intestinal length of Arab Chickens at two weeks of age. Keywords: Arab chickens, hatching egg, intestinal development, storage duration. Share Link | Plain Format | Corresponding Author (Faizal Andri)

63	Animal Reproduction and Breeding	ABS-9
Genetic Diversity Analysis of Exon-2 Growth Hormone (GH) Gene in Crossbred Chicken (Sentul x Arab) Using PCR-RFLP Technique Dika Irmaya.H (a), Depison(b), Ari Ardiantoro(a), and Suyadi (a*) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *suyadi[at]ub.ac.id b) Faculty of Animal Science, Universitas Jambi, Jambi 36361, Indonesia Abstract The research aims to analyze the genetic diversity of the GH gene using the PCR-RFLP technique. Fifty blood samples were collected from crossed Sentul and Arab Chickens aged three months. DNA was extracted from whole blood samples, and its quantity was evaluated by bio-drop technique. Extracted DNA was amplified for gene GH using a PCR machine with primer of forward (5^-GTAATGCAGCCACTTCTC-3^) and primer reverse (5^ GGCATCAAGCTAATGAGG-3^) GenBank AY461843. The PCR product was restricted by MspI (C&#8595-CGG) and Hae-III (GG&#8595-CC) enzymes for identifying the fragment length diversity. The variables observed in this study were the genotype and alleles of the obtained genotype. GH gene was amplified with primers of 519 bp, and the results show the monomorphic GH gene in this research. Both enzymes weren^t restricted to these amplification sites. Keywords: Crossbred chicken- Growth hormone gene- PCR-RFLP- Restriction enzym Share Link | Plain Format | Corresponding Author (Dika Irmaya. H)

64	Animal Reproduction and Breeding	ABS-10
Effects of sire breed on prolific chances and weaning weight of ewes Bramantyo Jusuf Sapa Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia bramantyojs[at]student.ub.ac.id Abstract This study was conducted to investigate the effects of the sire of a sheep^s breed along with their physical quality, which is weight, to see the correlations with the prolific capabilities of the sheep. One hundred twenty seven ewes were measured as to obtain their weaning weight and were reviewed based on past recordings wether they^re of singular birth or prolific. Measurements are also done towards their Sires and Dams to investigate the effects of parents^ weight on the prolific ewes qualities and their weight during the weaning period. Those lambs are mostly within weaning period to determine the correlation of weight and breed to how they were born under proliferative context. Using the average weaning weight of the lambs obtained from measurement, categorized by breed (DEG, Garut, Texel cross, and Merino cross) along with birth type (Single, Twins, Triplets), the results are summarized to several interpretations which reveals that crossbreeds generally have heavier weight but less prolific births compared to local breeds. Data analysis utilizing anova results that weaning weight is affected by birth type (P<0.05), but is nearly unaffected by breed (P < 0.5). The chances of prolific birth itself is also unaffected by breed (P<0.5). Keywords: breed, weaning weight, prolific Share Link | Plain Format | Corresponding Author (Bramantyo Jusuf Sapa)

65	Animal Reproduction and Breeding	ABS-17
Quantitative and Qualitative Broodstock of Thin-Tailed Sheep (DET) And Fat-Tailed Sheep (DEG) Andien Huli Afghani (1), Veronica Margareta Ani Nurgiartiningsih (2) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia andienhuliaf[at]student.ub.ac.id Abstract This study aims to determine the Quantitative and Qualitative Characteristic Broodstock of Thin-Tailed Sheep (DET) and Fat-Tailed Sheep (DEG). The study starts from first August 2023 until first December 2023 in PT Juara Agroniaga Sejahtera, Jepangrejo Village, Blora City District, Blora Regency. The material used in this study was 39 Thin-tailed Sheep and 28 Fat-tailed Sheep aged 1-2 years. The variables measured where Quantitative data were body height (BH), body length (BL), chest circumference (CC), body weight (BW), and Qualitative data were fur color and horn. Data were analysis using the pearson correlation, and regression method. The results of this study showed that the correlation between body height (BH), body length (BL), chest circumference (CC) with body weight (BW) in DET and DEG were 0.62 kg, 0.71 kg, 0.72 kg, 0.27 kg, 0.69 kg, 0.65 kg respectively. The results of this study showed that the regression between body height (BH), body length (BL), chest circumference (CC) with body weight (BW) in DET and DEG were BW = negative 14.03 approximately 0.71 BH, BW = negative 15.45 approximately 0.74 BL, BW = negative 21.95 approximately 0.65 CC and BW = 8.69 approximately 0.33 BH, BW = negative 6.76 approximately 0.59 BL, BW = negative 19.43 approximately 0.65 CC respectively. The results of this study showed that the dominant wool color pattern of DET is single white, 74.36% white, 15.38% black and 5.13% brown and DEG is single white, 67.86% white, 14.29% black and 7.14% brown. DET was 2.56% with horn then 97.44% without horn and DEG is 100% without horn. Keywords: Thin-tailed Sheep, Fat-tailed Sheep, Characteristic, Quantitative, Qualitative, Broodstock Share Link | Plain Format | Corresponding Author (Andien Huli Afghani)

66	Animal Reproduction and Breeding	ABS-47
Successful Artificial Insemination Using Frozen Semen From Embryo Transfer dan Proven Friesian Holstein Bull Ismail Hasan, Aulia Puspita Anugra Yekti, Hermanto, Nanang Febrianto, Rizki Prafitri, Sri Wahjuningsih, and Trinil Susilawati* a. Post Graduate Programe Faculty of Animal Science, Brawijaya University, Malang b. Lecturer Faculty of Animal Science, Brawijaya University, Malang Abstract This research aimed to know the success of artificial insemination using frozen semen from a proven bull and embryo transfer result. The research was conducted at the Public Dairy Farm Pujon District, Malang Regency, from August 13 to December 13, 2022. The material used in this research was 56 filial Friesian Holstein cows. Samples were divided into two treatments: 28 cows in single-dose artificial insemination with treatment T1 (proven bull) and 28 cows in single-dose artificial insemination with treatment T2 (embryo transfer result). Samples were selected by purposive sampling with the criteria of being at least 1.5 years old, having a Body Condition Score (BCS) of at least 2.5 (scale 1-5), having normal reproductive organs, showing signs of heat, and a mature body. AI was carried out with a single dose at the 8th hour, deposition 4 (corpus uteri), thawing at 22-23C for 60 seconds, and injection of vitamin ADE was given after the implementation of AI. The success of artificial insemination was evaluated using Non-Return Rate-1 (NRR-1), Non-Return Rate-2 (NRR-2), and Conception Rate (CR). The data were analyzed descriptively. The percentage of success for AI using frozen semen from a proven bull has NRR-1, NRR-2, and CR values of 96%, 75%, and 43%, while the percentage of success for AI using frozen semen embryo transfer results has a value of NRR-1, NRR-2, and CR were 92%, 85%, and 64%. The research results concluded that AI^s success using frozen semen from embryo transfer results was better than frozen semen from a proven bull. Keywords: Artificial Insemination, Conception Rate, Non Return Rate, Proven Bull Share Link | Plain Format | Corresponding Author (Ismail Hasan)

67	Animal Reproduction and Breeding	ABS-52
Correlation Between Morphometric Traits and Body Weight in Fat-Tailed Sheep Fajar Dwi Arvandi (a*), Veronica Margareta Ani Nugiartiningsih (b) a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *fajardwi4444[at]student.ub.ac.id b) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia Abstract This Study was conducted to determine the correlation between morphometric traits and body weight in fat-tailed sheep. The materials used this study were 80 Fat-Tailed sheep aged 90 days to 2 years. The variables measured were morphometric traits (body weight, body length, and chest circumference) and body weight. The data obtained were analyzed using pearson correlation and regression methods. The result showed that the correlation between morphometric traits and Body Weight (BW) of Fat-Tailed Sheep were as follows, 0.53 Body Height (BH), 0.55 Body Length (BL), and 0.71 Chest Circumference (CC). The results of this study indicate that the regression between morphometric traits and Body Weight (BW) of Fat-Tailed Sheep aged 90 days to 2 years were as follow: BW -12.38 +- 0.72 BH, BW -21.35 +- 0.88 BL, BW -10.78 +- 0.57 CC. The conclusion obtained is that the correlation between morphometric traits and body weight in Fat Tailed Sheep aged 90 days to 2 years, with correlation values ranging from 0.53 to 0.71 indicates a medium to strong correlation coefficient. CC has the highest correlation with BW compared to BH and BL. Keywords: Fat-Tailed Sheep, Morphometric Traits, Body Weight, Correlation Share Link | Plain Format | Corresponding Author (Fajar Dwi Arvandi)

68	Animal Reproduction and Breeding	ABS-55
The Effect of Synchronizing with the PGF2 alfa hormone on the Estrus Activity of Madura Cattle Muchamad Luthfi (1,2*), Lukman Affandhy S (1), Sri Wahyuningsih (3), Gatot Ciptadi (3) dan Trinil Susilawati (3*) (1)National Research and Innovation Agency, Indonesia (2) Doctoral Program Animal Science, University of Brawijaya, Malang, East Java, Indonesia (3) Faculty of Animal Husbandry, University of Brawijaya, Malang, East Java, Indonesia Abstract This research aims to determine the biological response of administering the PGF2&#945- hormone to the estrous activity of Madurese cows. This research method uses two observation techniques, namely estrus synchronization by administering the PGF2&#945- hormone Alfaglandin and observing natural estrus.. The data were analyzed by using the t test using the Software for Research statistix 10. The results showed that the frequency of estrus at the same time of observation was not significantly different from the two treatments (p>0.05), the highest frequency of estrus occurred at 18.00-20.00 in synchronized cows or not. The results of visualization of estrus showed cervical mucus, swollen vulva, and vaginal pH had the same percentage, while the vaginal temperature and duration of estrus had different percentages. The conclusion of this study is the frequency of estrous events, estrus visualization (cervical mucus discharge, swollen vulva, vaginal pH, and length of estrus), and the appearance of cervical ferning has the same response, while estrus temperature has a variable response. Keywords: Madura cattle, PGF2 alfa Hormone, Estrus Synchronization Share Link | Plain Format | Corresponding Author (Muchamad Luthfi)

69	Animal Reproduction and Breeding	ABS-62
Morphometric Traits of Black Soldier Fly Larvae in Slaughterhouse Waste and Rice Bran Growing Medium Fadilla Rizkia Fasha1, Dedes Amertaningtyas2, Faizal Andri2, Nurul Isnaini2,* 1Master Student, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia 2Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia *Corresponding author: nurulisna[at]ub.ac.id Abstract This study investigated the effects of utilizing a mixture of slaughterhouse waste (SW) and rice bran (RB) as a growing medium on the morphometric traits of Black Soldier Fly (BSF) larvae. The research followed a completely randomized design with five treatment groups, namely T0: 100% SW (control), T1: 75% SW + 25% RB, T2: 50% SW + 50% RB, T3: 25% SW + 75% RB, and T4: 100% RB. The morphometric traits examined in this study included the larvae body length at 19 days (LBL19), larvae body width at 19 days (LBW19), larvae body length at 29 days (LBL29), and larvae body width at 29 days (LBW29). The results demonstrated that using SW as the sole medium (T0) could effectively support the growth of BSF larvae, but the morphometric traits of larvae in T0 were relatively modest. Interestingly, when RB was used to partially replace SW, it had a dose-dependent effect. T1 led to significant improvements (P<0.05) in LL19, LL29, and LW29. Conversely, the morphometric traits of BSF larvae in T2 and T3 did not exhibit significant changes (P>0.05) compared to the control. On the other hand, the use of T4 significantly enhanced (P<0.05) all morphometric traits of BSF larvae (LL19, LW19, LL29, and LW29). In conclusion, this study reveals the significant potential of the Black Soldier Fly in managing slaughterhouse waste. Moreover, substituting 25% of the slaughterhouse waste with rice bran can be considered an effective growing medium for enhancing the morphometric traits of Black Soldier Fly larvae. Keywords: Bioconversion, Black Soldier Fly, Slaughterhouse Waste, Sustainable Waste Management Share Link | Plain Format | Corresponding Author (Nurul Isnaini)

70	Animal Reproduction and Breeding	ABS-65
Polymorphism of IGFBP7 gene (g.72351183 A>C) and its association with mineral content and cholesterol of Indonesian lamb meat Komarudin1,2, Kasita Listyarini3, Cahyo Budiman3, Cece Sumantri3, and Asep Gunawan3,* 1Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia 2Indonesian Agency for Agricultural Instrument Standardization, Ministry of Agriculture, Indonesia 3Department of Animal Production and Technology, Faculty of Animal Science, IPB University, Bogor, Indonesia Abstract The genetic improvement of sheep for higher productivity and meat quality is strongly needed due to the increasing demand for meat sheep. Insulin growth factor binding protein 7 (IGFBP7) has been reported as a potential gene which has a substantial role in meat quality. The objective of the study was to identify IGFBP7 gene polymorphisms and their association with minerals and cholesterol in Indonesian lamb meat. The study used 130 rams consisting of local and crossbred sheep. The local sheep were Javanese thin-tailed, Javanese fat-tailed, and Jonggol sheep. The crossbred sheep were Compass agrinak, Composite garut agrinak and Bahtera agrinak sheep. The identification of polymorphism was using PCR-RFLP method. The association analysis was obtained using a general linear model. The results showed that IGFBP7 (g.72351183 A>C) was polymorphic in local and crossbred sheep. The IGFBP7 was significantly associated with cholesterol in crossbred (P<0.01), whereas the AA genotype had the lowest cholesterol. Cholesterol in crossbred sheep also was higher (P<0.01) compared to local sheep for all observed genotypes. IGFBP7 gene was not significantly associated with minerals and cholesterol (P>0.05) in local sheep. It is concluded that IGFBP7 gene seems promising as a genetic marker for cholesterol level in Indonesian crossbred sheep. Keywords: IGFBP7 gene, mineral, cholesterol, lamb meat Share Link | Plain Format | Corresponding Author (Komarudin Komarudin)

71	Animal Reproduction and Breeding	ABS-75
Quality and Proportion of Sexed Spermatozoa Using Percoll Density Centrifugation Method at Different Gradients with AndroMed Chairun Nisa Aperi Simbolon (a), Anggita Dian Pramudhita (b), Rr. Ani Rizqianti (b), Aulia Puspita Anugra Yekti (c), Nurul Isnaini, and (c) Trinil Susilawati (c*) a) Postgraduate Student Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia b) Undergraduate Student Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia c) Lecture Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia, *Email : tsusilawati[at]ub.ac.id Abstract The utilization of sexing technology is an effort made to improve the efficiency of livestock farming, which was created to predict the sex of the puppies born so that it can be adjusted to the objectives of the farm. This study aims to analyze the quality and proportion of sexed spermatozoa using the percoll density centrifugation method at different gradients with AndroMed diluent. The research material used was fresh semen from 1 Belgian Blue cow aged six years with a body weight of 600 kg and fresh semen quality motility higher than or equal to 70%. This research is a laboratory experiment with two treatments and ten replicates. The treatments in the study were T0: 10 Gradient density percoll + AndroMed and T1: 5 Gradient density percoll + AndroMed. The research analysis used a dependent t-test. The statistical analysis showed a significant difference in treating ten gradients with five gradients in each layer regarding individual motility, viability, abnormality, and total motile spermatozoa. At the same time, in concentration, there was no difference in the treatment of 10 gradients with five gradients. The average results on individual motility, abnormality, concentration, and total motile spermatozoa showed treatment of 10 gradients better than treatment of 5 gradients in each layer. At the same time, the variable viability showed that the gradient treatment is better than the five-gradient treatment. Proportion of spermatozoa in the Upper Layer T0 X (23.4%), Y (76.8%) and T1 X (16.9%), Y (83.1%). The proportion of Spermatozoa in the Bottom Layer T0 X (84.1%), Y (15.9%), and T1 X (83%), Y (17%), respectively. In conclusion, sexing spermatozoa with the Percoll 10 and 5 Gradient Density Centrifugation method can separate X and Y spermatozoa. Keywords: AndroMed, Bovine, Percoll density centrifuge, Sperm Sexing, Sperm Quality Share Link | Plain Format | Corresponding Author (Chairun Nisa Aperi Simbolon)

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