The effectiveness of using a combination of saponin and vaccine given in ovo on hatchability and early growth performance of local chickens
Rantan Krisnan (a*), Cecep Hidayat (a), Astu Unadi (a), Isbandi (a), Rusdiana Supardi (a), Simon P. Ginting (a), Bachtar Bakrie (a), Chalid Talib (a), Andi Tarigan (a), Mohammad Ikhsan Shiddieqy (a), Muhamad Hamdan (a), Andi Suhandi (a), Winwin Widaringsih (a)

a*) Research Organization for Agriculture and Food, National Research and Innovation Agency
a) Research Organization for Agriculture and Food, National Research and Innovation Agency


Abstract

Providing nutrients as early as possible (in ovo feeding) to local chickens is an important breakthrough for the development of poultry businesses in Indonesia, because it will be a big opportunity to produce superior local chicks (day old chick) that has been enriched with important nutrients before hatching. A study has been carried out to determine the correct dosage for using a combination of saponin and vaccine in an in ovo formula on hatchability and early growth performance. There were four levels of saponin use (0.0- 1.5- 3.0- 4.5 mikrogram) arranged using a factorial design with two factors, namely saponin level as the first factor, while whether or not vaccine was used as the second factor, so there were eight formula combinations. Each formula combination was dissolved in phosphate buffered saline, then injected into the amnion of fertile eggs of Sentul chickens during incubation for 18 days (0.5 ml/egg) using an automatic injector machine with a 20G needle (diameter 0.9 mm and length 8 mm). The results showed that the hatchability of Sentul chickens ranges from 66 until 93%. The combination of vaccinated formula containing 0.0 mikrogram saponin had the highest hatchability value (93.33%) and the best body weight gain and feed conversion. This formula combination was significantly different (P<0.05) from other formula combinations. A trend was seen that increasing the dose of saponin used showed a decrease in the performance of early growing chickens. This happens in combination formulas with vaccines or without vaccines

Keywords: in ovo, saponin, vaccines, hatchability, early growth, local chicken

Topic: Animal Nutrition

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