The Potential of Frozen Rumen Fluid with Dimethyl Sulfoxide And Glucose As Cryoprotectant on In Vitro Gas Production And Digestibility Sayyid Muhammad Djafar(a*), Divia Sekar Ayu Putri Darmawan(a), Adam Suryapratama(a), Hendrawan Soetanto(a)
a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
*sayyid.muhammad.djafar[at]gmail.com
Abstract
As the pressure from the animal welfare community increases there is an urgent need to find the alternative rumen fluid (RF) source as an essential inoculum for in vitro digestibility studies. The objective of this study is to support the previous studies utilizing fresh RF from abattoir with and without cryoprotectants during the freezing process. A 2x3x3 factorial design was used to ascertain the effectiveness of dimethyl sulfoxide (DMSO) and glucose as cryoprotectants to frozen RF varying from 4, 11, and 18 days to measure the in vitro gas production (IVGP) and digestibility of a complete feed consisted of elephant grass and commercial concentrate at 1 : 1 ratio. factor A being the preservation methods (A: immediate & B: gradual freezing), factor B being the cryoprotectants (P0: None | P1: 1% DMSO | P2: 1% DMSO + 0.8% Glucose), and factor C being the preservation time (4, 11, and 18 days). Experimented on in vitro gas production (IVGP) and digestibility (IVD), the analysis carried out mixed results. IVGP shows that the use of DMSO and glucose as cryoprotectants (P1A, P1B, P2A, & P2B) only held back quality performance and made the non-cryoprotected frozen RF (P0A) is the most applicable method for preservation that lasts until 18 days. IVD however resulted the most similar frozen RF treatment with gradual freezing that use DMSO as cryoprotectant (P1B). Thus making DMSO and glucose influenced nothing significant to the frozen RF. Maintaining the reduction of animal welfare while simultaneously keeping the data variation low is achievable and efficient.
Keywords: Cryoprotectant, DMSO, Glucose, In Vitro, Rumen Fluid
