Effects of Dimethyl Sulfoxide As Cryoprotectant For Rumen Fluid On In Vitro Gas Production And Volatile Fatty Acid Content Sayyid Muhammad Djafar(a*), Alfina Salsabila(a), Aksauri Hana Malis(a), Hendrawan Soetanto(a)
a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
*sayyid.muhammad.djafar[at]gmail.com
Abstract
Initiating the research hypothesis of dimethyl sulfoxide(DMSO) potential for preserving the rumen fluid(RF), this experiment^s urgency is based on welfare that seems violated when fistulated cattle used regulary for the inoculum source. The alternative was experimented by taking loads of rumen fluid from Madura cattle from slaughterhouse, to prevent welfare violations by rumen fluid extraction, and to reduce data variation since only single inoculum source used. The in vitro gas production enrolled by 2x2x3 factorial design, with a couple freezing treatments in -21 C using 5% DMSO and unvarnished as the factor A, which complemented by standard and complete feed each as the factor B. They were preserved for 2, 5, and 7 days as the factor C. Post-analysis results show that preservation time on unvarnished differed significantly with total gas produced (P>0.01), and did not impacted on the potential (P<0.05) and the rate (P<0.05) of gas production. On the other hand, specific volatile fatty acid content of the treated RF shows that DMSO impacted significantly on butyric acid (P>0.01), differed on propionic acid (P>0.05), and did not alter anything on acetic acid (P<0.05). By having more volatile fatty acid on the DMSO RF, the potential gas produced is not as high as the unvarnished, thus making the addition of DMSO to the RF is not benefited the preservation yet. On the flipside the potency of unvarnished RF to be preserved is applicable for reusability.
Keywords: DMSO, In Vitro Gas Production, Rumen Fluid, Volatile Fatty Acid
