Exploration of gram - negative bacterial contamination edible bird nest in bone district
Dhiya Nabilah Jafar, Baso yusuf, A. Magfirah Satya Apada, Rizalinda Sjahril, Sitti Arifah

Hasanuddin University

Abstract

Edible nest swiftlet (Aerodramus fuciphagus) is a type of bird that produces high economic nest that made from its saliva. The nest has many benefits and a very delicious taste, but the edible bird nest which is a food product of animal orginin has a risk of harmful microbial contamination for human health. This study aims to determine the presence of bacterial contamination found in walet nests and determine the types of bacteria found in it. In this study the sample used was taken from walet bird houses in 5 different regions in Bone. Bacterial identification was carried out at the Microbiology Laboratory of Makassar City Health Office. Sample from each region were taken as much 5,6 gram and then dissolved into 50 ml of the fertilizer solution Brain-heart Infusion Broth (BHIB). Then the colonies that had grown in Brain-heart Infusion Broth BHIB were inoculated on Mac Conkey agar media. After that, Colonies on Mac Conkey is taken and put into a tube containing 3 ml of suspense and then homogenized. Then the turbidity test was carried out using Densicheck until it reached 0.5 Mcfarland and put into Vitek 2 compact system. The results showed that samples from rural areas identified Pseudomonas aeruginosa. The samples from the urban areas identified Proteus mirabilis. Samples from mountainous areas identified Escherichia coli. Samples from marine areas identified Enterobacter aeruginosa. And samples from rice fields identified Enterobacter cloaca complex.

Keywords: Bacteria, Vitek 2 compact, edible bird nest

Topic: Zoonotic Disease and Tropical Disease