Genetic Diversity Analysis of Exon-2 Growth Hormone (GH) Gene in Crossbred Chicken (Sentul x Arab) Using PCR-RFLP Technique
Dika Irmaya.H (a), Depison(b), Ari Ardiantoro(a), and Suyadi (a*)

a) Faculty of Animal Science, Universitas Brawijaya, Malang 65145, Indonesia
*suyadi[at]ub.ac.id
b) Faculty of Animal Science, Universitas Jambi, Jambi 36361, Indonesia

Abstract

The research aims to analyze the genetic diversity of the GH gene using the PCR-RFLP technique. Fifty blood samples were collected from crossed Sentul and Arab Chickens aged three months. DNA was extracted from whole blood samples, and its quantity was evaluated by bio-drop technique. Extracted DNA was amplified for gene GH using a PCR machine with primer of forward (5^-GTAATGCAGCCACTTCTC-3^) and primer reverse (5^ GGCATCAAGCTAATGAGG-3^) GenBank AY461843. The PCR product was restricted by MspI (C&#8595-CGG) and Hae-III (GG&#8595-CC) enzymes for identifying the fragment length diversity. The variables observed in this study were the genotype and alleles of the obtained genotype. GH gene was amplified with primers of 519 bp, and the results show the monomorphic GH gene in this research. Both enzymes weren^t restricted to these amplification sites.

Keywords: Crossbred chicken- Growth hormone gene- PCR-RFLP- Restriction enzym

Topic: Animal Reproduction and Breeding