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Successful Artificial Insemination Using Frozen Semen From Embryo Transfer dan Proven Friesian Holstein Bull a. Post Graduate Programe Faculty of Animal Science, Brawijaya University, Malang Abstract This research aimed to know the success of artificial insemination using frozen semen from a proven bull and embryo transfer result. The research was conducted at the Public Dairy Farm Pujon District, Malang Regency, from August 13 to December 13, 2022. The material used in this research was 56 filial Friesian Holstein cows. Samples were divided into two treatments: 28 cows in single-dose artificial insemination with treatment T1 (proven bull) and 28 cows in single-dose artificial insemination with treatment T2 (embryo transfer result). Samples were selected by purposive sampling with the criteria of being at least 1.5 years old, having a Body Condition Score (BCS) of at least 2.5 (scale 1-5), having normal reproductive organs, showing signs of heat, and a mature body. AI was carried out with a single dose at the 8th hour, deposition 4 (corpus uteri), thawing at 22-23C for 60 seconds, and injection of vitamin ADE was given after the implementation of AI. The success of artificial insemination was evaluated using Non-Return Rate-1 (NRR-1), Non-Return Rate-2 (NRR-2), and Conception Rate (CR). The data were analyzed descriptively. The percentage of success for AI using frozen semen from a proven bull has NRR-1, NRR-2, and CR values of 96%, 75%, and 43%, while the percentage of success for AI using frozen semen embryo transfer results has a value of NRR-1, NRR-2, and CR were 92%, 85%, and 64%. The research results concluded that AI^s success using frozen semen from embryo transfer results was better than frozen semen from a proven bull. Keywords: Artificial Insemination, Conception Rate, Non Return Rate, Proven Bull Topic: Animal Reproduction and Breeding |
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