CHARACTERISTICS OF NANOPARTICLES Caulerpa racemosa AS AN ANTI-BREAST CANCER AGENT Sri Haryanti, Dewi Ramonah, Mutmainah
Stifar Yayasan Pharmasi Semarang
Abstract
Marine algae or Caulerpa racemose contains caulerpenin which have anticancer properties. The use of natural ingredients often fails in the clinical phase due to low bioavailability. Nanotechnology offers advantages in increasing bioavailability.
This research aims to determine the physical characteristics of nanoencapsulation of Caulerpa racemose extract with three dosage levels of 100 mg/mL- 125 mg/mL- and 150 mg/mL and determine the compound content of Caulerpa racemose extract.
The extract was made by maceration for 3x24 hours. Extract nanoparticles were made by mixing 2.3 mL of Caulerpa racemose extract- 2.5 mL of twee 80, 0.3% chitosan solution in acetate buffer pH 4 added with TPP solution in distilled water (concentration 00.1%). Test the particle size using the Particle Size Analyzer tool. The zeta potential and polydispersity index were measured using a Particle Size Analyzer.
The results of phytochemical screening showed that Caulerpa racemose extract positively contained flavonoids, alkaloids, terpenoids, and phenolics. The results of the physical characteristics of nanoencapsulation of Caulerpa racemose extract with three dosage levels of 100 mg/mL- 125 mg/mL- and 150 mg/mL show a larger particle size, but still in the nano range. From the research results, the higher the dose, the smaller the zeta potential value, but less than +30 mV. Meanwhile, the polydispersity index value had the highest results at a dose level of 125 mg/mL.
Through anticancer testing, it is hoped that Caulerpa racemose extract nanoparticle preparations can be used as an anticancer agent
