Formulation and Physical Characteristics Test of Chlorophyll-c Emugel Preparation from Brown Seaweed (Sargassum polycystum) Lia Kusmita*, Dinara Safina Firdaus Syifa^, Mutmainah
Stifar Yayasan Pharmasi Semarang, Letjend Sarwo Edhie Wibowo KM 1 Plamongansari Pucanggading Semarang
*email: lia_kusmita[at]yahoo.com
Abstract
The abstract should summarize the contents of the paper in short terms, i.e. 150-250 words Brown seaweed (Sargassum polycystum) contains a distinctive pig-ment, chlorophyll-c. Chlorophyll is known to exhibit strong antibacterial activity against gram-positive bacteria. Emulgel is one of the topical preparations that of-fers several advantages, including being non-greasy, easy to spread, easy to clean, soft, easy to wash, having a longer shelf life, transparent and comfortable when used. The purpose of this study was to formulate chlorophyll-c isolate in the form of an emulgel preparation to be tested for physical characteristics, anti-oxidant activity, and irritation tests on rabbits. The research was conducted by isolating chlorophyll-c using column chromatography and preparative thin layer chromatography (PTLC) methods. Emulgel dosage formulations were created with different chlorophyll-c content as the active substance, consisting of FI 0.2%, FII 0.4%, and FIII 0.6%. The results showed that there was an influence of chlorophyll-c concentration on the characteristics of the emulgel preparation. The higher the concentration of chlorophyll-c, the greener the color of the emulgel, the pH decreases, the spreadability decreases, but the viscosity and stickiness increase. Testing of chlorophyll-c emulgel on antioxidant activity showed that the higher the concentration of chlorophyll-c, the greater the antioxi-dant activity. Giving chlorophyll-c emulgel to rabbits causes very mild irritation with the appearance of erythema or redness and edema that is not very clear.
