EFFECT OF NFKΒ-, CASPASE-3, AND E-SELECTIN ON PREGNANT RATS TOXOPLASMOSIS MODEL Hafi Nurinasari1, Sajidan1, Bambang Purwanto1, Dono Indarto1, Didik T Subekti,23
1Doctorate Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Biology Study Program, Faculty of Education, Universitas Sebelas Maret, Surakarta, Indonesia
Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
Department of Physiology and Biomedical Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia
2The Center of Veterinary Research (BBALITVET) Bogor
3 Biomedical Research Center, National Research and Innovation Center (BRIN)
Abstract
Introduction: Toxoplasmosis is a zoonotic disease caused by the obligate intracellular Toxoplasma gondii that can infect humans and warm-blooded animals. The prevalence of toxoplasmosis occurs in most areas of the world and about one third is estimated to be chronically exposed to toxoplasmosis. T. gondii infection causes oxidative stress that causes an imbalance between the free radical-producing system and the cellular defense system, namely an increase in free radical production or a decrease in antioxidant defense activity or both. Oxidative stress and infection will cause inflammation there by increasing levels of Nuclear Factor Kappa beta, caspaae 3 and E-Selectin resulting in inflammation and cell death.
Objective: The purpose of this study was to to determine the effect of NFKΒ-, caspase-3 and E-Selectin on pregnant rats model of toxoplasmosis.
Materials and Methods: This research is a laboratory experimental study using Randomized Control Trial (RCT) with posttest only with control group design. This study used female rats (Rattus novergicus) aged 6-8 weeks, weighing 150-200 grams. In this study, female rats that had gone through the pregnancy process were divided into four groups, namely one control group which was not given Toxoplasma isolate, and the other three groups were differentiated based on the dose of Toxoplasma isolate given, namely 10, 100, and 1000 tachyzoites. Each group consisted of four rats. Toxoplasma isolate was administered intraperitoneally as much as 2 ml on the 12th day of pregnancy and then measured levels of NFKΒ-, caspase-3 and E-Selectin through rat blood samples on the 17th day of gestation.
Results: There were significant differences in the levels of NFKΒ- (p < 0.001), caspase-3 (p < 0.001 and E-Selectin (p < 0.001) in the four groups. The data mean difference in levels of NFKΒ-, caspase-3 and E-Selectin in the four groups showed the increase corresponds to an increase in the dose of Toxoplasma tachyzoite injected in mice.
Conclusion: There was a significant difference in the increase in levels of NFKΒ-, caspase-3 and E-Selectin in pregnant toxoplasmosis model rats along with an increase in the dose of toxoplasma tachyzoites compared to normal pregnant rats.
Keywords: Toxoplasma gondii, NFKΒ- , caspase- 3, E- Selectin,, Pregnant rats
Keywords: PleaToxoplasma gondii, NFKΒ- , caspase- 3, E- Selectin,, Pregnant ratsse Just Try to Submit This Sample Abstract
Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous
