The Shrimp Skin Extract (Litopenaeus vannamei) as an adjunct Therapy of Hemostasis in Open Fractures Ratna Indriawati1*, Alifia Ichsan Nabila 2
Universitas Muhammadiyah Yogyakarta
Abstract
Shrimp is the main export commodity of Indonesian fisheries. The majority of shipments are in the form of fresh frozen shellless shrimp, which results in increased shrimp shell waste. The utilization of waste is still lacking even though shrimp shells have many important ingredients, one of which is chitosan. Chitosan has been shown to be able to accelerate hemostasis. This study aimed to examine the shrimp skin extract as adjunct therapy of hemostasis in hemostasis through a clotting time (CT) test in open fractures. This study was an experimental study with 18 samples of white rats (Rattus norvegicus) induced by fracture, divided into 3 groups, control, oral chitosan, and topical chitosan. The control group did not receive chitosan therapy, but all groups received levofloxacin injections and splint dressing. The clotting time test using the Lee-White method was held on days 1, 3, and 5 by taking venous blood through the orbital sinus, for each clotting time test, 2 samples were taken from each group and euthanized immediately after blood collection. The data obtained were analyzed using Kruskal-Wallis. The results showed that there was a decrease in CT time although it was not statistically significant in the CT test on day 1 (p 0.156), day 3 (p=0.102), and day 5 (p=0.368). There was a significant improvement in swelling found in the topical chitosan group. The shrimp skin extract as an adjunct therapy did not significantly accelerate clotting time in open fractures.
Keywords: Shrimp shell, hemostasis, clotting time, chitosan, fracture
Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous
