The Immunomodulator Effective Dose of Nanosilver Biosynthetic based on Immunoglobulin G Level of Mice that Induced by Vaccine Compared with Herbal Immunostimulant Dian Eka Ermawati,Putri Indah Nuraini,Niki Putri Anjani,Adi Yugatama
Universitas Sebelas Maret, Surakarta,Indonesia
Abstract
Immunization is an effort to increase a person^s immunity against a disease actively. The process of the formation of antibodies by the body after immunization requires complementary treatment for immunostimulators. Silver in the medical field developed as a therapeutic agent. When in the form of nanoparticles, it can reduce toxicity and act as an immune catalyst. Inulin acts as a bioreductant to synthesize silver because it is more environmentally friendly than physical and chemical methods. Nanosilver biosynthetic (AgNPs) from inulin must be verified as an immunomodulatory agent. This study aims to determine the effective dose of nanosilver based on Immunoglobulin G of Balb/c mice induced by the measles vaccine compared to herbal immunostimulants and their stability in 21 days. The research method was experimental in the laboratory. AgNPs biosynthesis process using inulin gembili as a bioreductant, then characterize using spectrophotometer UV-VIS and PSA, animal conditioning, treatment of an animal, ELISA test, and stability test of nanosilver. The data were processed using SPSS 21 and analyzed statistically using One Way ANOVA and Wilcoxon test. The results of the ELISA readings showed an increase in the Optical Density value of the nanosilver sample at a dose of 4 mg/kg.BW was higher than the other treatments but not significantly different from the herbal stimulant control. There is an effect of storage time on the stability of nanosilver during a storage period of 21 days at a temperature of 4 degree celcius on the maximum lambda shift. However, it is still in the nanoparticle size range. In addition, there is a change in the color of the solution from light brown to dark brown, which indicates that a nanosilver formation process has continued.
