Comparison of Administration of Lipopolysaccharide (LPS) with Isoproterenol on Fibrosis Area in Sprague Dawley Rat: A Study of Acute Myocardial Infarct Induction in Rat Model Saugi Abduh (a,b*), Purwanto Bambang (c), Dirgahayu Paramasari (d), Soetrisno (e)
a)Student of Medical Science, Medical Faculty, Universitas Sebelas Maret, Surakarta, INDONESIA.
b)Division of Cardiology, Department of Internal Medicine Science, Sultan Agung Islamic University, Semarang, INDONESIA.
*drsaugiabduh01[at]gmail.com
c)Division of Kidney and Hypertension, Department of Internal Medicine Science, Universitas Sebelas Maret, Surakarta, INDONESIA.
d)Department of Parasitology, Universitas Sebelas Maret, Surakarta, INDONESIA.
e)Department of Obstetrics and Gynecology, Universitas Sebelas Maret, Surakarta, INDONESIA.
Abstract
Background: Myocardial infarct induction in rat model with isoproterenol is a standard in a cardioprotective research on various test substances. The other myocardial infarct induction model uses Lipopolysaccharide (LPS). LPS and isoproterenol show the same mechanism of induction of myocardial damage, that is disturbed balance in free radical formation and antioxidative resistance system. Objective: This research aims to examine the difference between LPS and Isoproterenol injections on rat model based on fibrosis area. Method: This research used a posttest only control group design with 15 sprague dawley rats as samples which were divided into 4 groups: NaCl control group, LPS 10 mg/mL group, LPS 15 mg/mL group, and isoproterenol group. The LPS and isoproterenol groups were respectively given intraperitoneal injection with LPS and isoproterenol for 14 days, while the control group was left without any treatment. On day 15 they were terminated and measured for fibrosis area histopathologically using micrometer. Result: This research shows that the infarct area of LPS 10mg/mL and 15mg/mL groups was 51.910μ-m2 and 102.512μ-m2, while that of the isoproterenol group was narrower of 21.190 μ-m2. Conclusion: LPS 15mg/mL induction causes wider myocardial fibrosis than LPS 10mg/mL induction and even than isoproterenol induction.
